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-Cellstain-DAPI Solution

Item # Unit Size
D523-10
1 ml

For Research Use Only Products

MSDS
Chemical Name: 4',6-Diamidino-2-phenylindole, dihydrochloride, aqueous solution [CAS#: 28718-90-3]

Product:
2.9 mM DAPI buffer solution
            (1 mg DAPI per 1 ml buffer)
Appearance: Slightly yellow to yellow liquid
MW: 350.25, C16H17Cl2N5

Storage Condition : 0-5ºC, protect from light
Shipping Condition : ambient temperature


Product Description
DAPI is an AT-sequence specific DNA intercalator that attaches to DNA at the minor groove of the double helix like Hoechst dyes. Though DAPI is not permeable through viable cell membranes, it passes through disturbed cell membranes to stain the nucleus. DAPI has a high photo-bleaching tolerance level. DAPI is utilized for the detection of mitochondrial DNA in yeast, chloroplast DNA, virus DNA, micoplasm DNA and chromosomal DNA. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.

Chemical Structure



Staining Procedure
  1. Prepare 10-50 μM DAPI solution with PBS or an appropriate buffer.a)
  2. Add DAPI solution with 1/10 of the volume of cell culture medium to the cell culture.b)
  3. Incubate the cell at 37oC for 10-20 min.
  4. Wash cells twice with PBS or an appropriate buffer.
  5. Observe the cells using a fluorescence microscope with 360 nm excitation and 460 nm emission filters.
a) Since DAPI may be carcinogenic, extreme care is necessary during handling.
b) Or you may replace the culture medium with 1/10 concentration of DAPI buffer solution.

Reference

1. W. Schnedl, et al., DIPI and DAPI: Fluorescence Banding with Only Negligible Fading. Hum Genet. 1977;36:167-172.
2. I. W. Taylor, et al., An Evaluation of DNA Fluochromes, Staining Techniques, and Analysis for Flow Cytometry. I. Unperturebed Cellpopulations. J Histochem Cytochem. 1980;28:1224-1232.
3. F. Otto, et al., A Comparative Study of DAPI, DIPI, and Hoechst 33258 and 33342 as Chromosomal DNA Stains. Stain Technol. 1985;60:7-11.
4. N. Poulin, et al., Quantitative Precision of an Automated Image Cytometric System for the Measurement of DNA Content and Distribution in Cells Labeled with Fluorescent Nucleic Acid Stains. Cytometry. 1994;16:227-235.
5. M. Kawai, et al., Rapid Enumeration of Physiologically Active Bacteria in Purified Water Used in the Pharmaceutical Manufacuturing Process. J Appl Microbiol. 1999;86:496-504.

Fig. 1 Cell staining with DAPI