Chemical Name: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide, aqueous solution
Product: 1.5 mM PI aqueous solution
(1 mg PI per 1 ml water)
Appearance: Orange to red solution
MW: 668.39, C27H34I2N4
Storage Condition : -20ºC, protect from light
Shipping Condition : ambient temperature
Propidium iodide (PI) is an ethidium bromide analog that emits red fluorescence upon intercalation with double-stranded DNA. PI does not permeate viable cell membranes, but passes through disturbed cell membranes and stains the nuclei. PI is often used in combination with a fluorescein compound, such as Calcein-AM or FDA, for simultaneous staining of viable and dead cells. The excitation and emission wavelengths of PI-DNA complex are 535 nm and 615 nm, respectively.