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8-Nitroguanine (lyophilized)

Item # Unit Size
N455-10
100 ug

For Research Use Only Products

Application: Blocking agent for immunostaining with Anti-Nitroguanosine
                       Antibody, standard of 8-Nitroguanosine EIA

MSDS

Appearance: Pale yellow powder
Purity: ≥90.0% (HPLC)
MW: 196.12, C5H4N6O3

Storage Condition: 0-5°C
Shipping Condition: ambient temperature

Product Description
8-Nitroguanine is a nitrated base of DNA and RNA. It is formed by peroxynitrite, which is generated from nitric oxide and superoxide anion radical. It is known that a large amount of nitric oxide molecules and superoxide anion, generated by inflammation, causes nitration of guanosine. Since chemically modified nucleotides cause mutation during DNA replication, 8-Nitroguanine is thought to be a marker of DNA damage related to mutation and cancer. 8-Nitroguanine (lyophilized) is made by the lyophilization of its phosphate-buffered saline solution and is used in immunohistochemistry for absorption testing. Adding 0.4 ml of distilled water to the 8-Nitroguanine powder produces a 1.2 mmol / l of 8-Nitroguanine solution, which is stable for one month at 4°C. If an antibody pre-treated with excessive 8-Nitroguanine shows negative staining, then the subsequent positive staining with this antibody will be specific for 8-nitroguanine or 8-nitroguanosine formed in DNA or RNA.

Chemical Structure



References
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