ChemicalName:1-[2-[5-(Dimethylamino)-1-naphthalenesulfonamido]ethyl]-1,4,7,10,-tetraazacyclo-dodecane,tetrahydrochloride, dihydrateCAS: 184537-03-9
Appearance: slightly yellow crystalline powder
Purity: ≥95.0% (HPLC)
MW: 630.50, C22H40Cl4N6O2S. 2H2O
Storage Condition: -20ºC,
Shipping Condition: ambient temperature
Dansylaminoethyl-cyclen is a water-soluble and cell-membrane-permeable fluorescent zinc indicator developed by Dr. E. Kimura. The aqueous solution of this reagent allows intracellular zinc ion to be monitored. The fluorescence intensity of the complex is 5 times that of the free ligand. Most other cations do not form fluorescent complexes with this reagent. Although the cadmium ion forms a fluorescent complex, the stability constant of this complex is one tenth that of the zinc complex. Thus, cadmium ion does not affect zinc detection in most cases. Copper ion (Cu(II)), which forms a non-fluorescent complex, also does not affect zinc detection because it is extremely rare as a free ion in normal living cells. Recently, the zinc ion has gained a lot of attention; it is thought to play an important role in ischemia and subsequent selective neuronal cell death. Reactive oxygen species might damage cystein-residues in a zinc-finger motif to produce free zinc ions.
1. T. Koike, et al., A Novel Biomimetic Zinc(II)-fluorohphore, Dansylamidoethyl-pendant Macrocyclic Tetraamine 1, 4, 7, 10-Tetraazacyclododecane (Cyclen). J Am Chem Soc. 1996;118:12696-12703.
2. E. Kimura, et al., A Macrocyclic Zinc(II) Fluorophore as a Detector of Apoptosis. PNAS. 2003;100:3731-3736.