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Zinquin ethyl ester

Item # Unit Size
Z215-10
1 mg

For Research Use Only Products

MSDS
ChemicalName:Ethyl [[2-methyl-8-[[(4-methylphenyl)sulfonyl]amino]-6-quinolinyl]oxy]acetate
CAS: 151606-29-0

Appearance: white or slightly blue crystalline powder
Purity: ≥98.0% (HPLC)
MW:
414.48, C21H22N2O5S

Storage Condition: -20ºC
Shipping Condition:
ambient temperature

Product Description
Zinquin ethyl ester is an analog of the widely used indicator TSQ. Although Zinquin ethyl ester itself is fluorescent, its fluorescence intensity is negligibly weak (1/30). Zinquin ethyl ester is membrane permeable, as are acetoxymethyl esters of calcium probes such as Fura 2-AM and Fluo 3-AM. Zinquin ethyl ester is thus useful to detect intracellular zinc ions. It forms a complex with a zinc ion with nitrogen atoms in the structure. This compound also forms a fluorescent complex with cadmium ion; however, detectable amounts of cadmium ions are not contained in normal living cells. Because the water solubility of Zinquin ethyl ester is poor, dimethylsulfoxide (DMSO) or ethanol is required as a solvent for preparing the stock solution.

Zinc Chelation


General Protocol for Lymphoblastoid Cells*
Reagents:
2.4 mM Zinquin ethyl ester stock solution (1 mg Zinquin ethyl ester in 1 ml DMSO) Zinquin ethyl ester stock solution is stable for 1 month at -20ºC.

Protocol:
  1. Suspend cells in Hanks Ebalanced salt solution (HBSS) to prepare 5-10 x 106 cells per ml medium.
  2. Add Zinquin ethyl ester stock solution to the cell suspension to prepare 2.4 μM Zinquin ethyl ester (1/1000 vol of the cell suspension) as the final concentration.
  3. Incubate the cell suspension at 37ºC for 30 min.
  4. Wash the cells 3 times with HBSS and then prepare 2-5 x 106 cells per ml of cell suspension.
  5. Determine the fluorescence intensity of each cell using a fluorescence microscope or a confocal laser microscope coupled with an image analyzer.
* Cell staining conditions depend on cell type, so it is necessary to optimize the conditions for each experiment.

References

1. P. Coyle, et al., Measurement of Zinc in Hepatocytes by Using a Fluorescent Probe, Zinquin: Relationship to Metallothionein and Intracellular Zinc. Biochem J. 1993;303:781-786.
2. P. D. Zalewski, et al., Correlation of Apoptosis with Change in Intracellular Labile Zn(II) Using Zinquin [(2-Methyl-8-p-Toluensulphonamido-6-Quinolyloxy)-Acetic Acid], a New Specific Fluorescent Probe for Zn(II). Biochem J. 1993;296:403-408.
3. P. D. Zalewski, et al., Video Image Analysis of Labile Zinc in Viable Pancreatic Islet Cells Using a Specific Fluorescent Probe for Zinc. J Histochem Cytochem. 1994;42:877-884.
4. P. Coyle, et al., Measurement of Zinc in Hepatocytes by Using a Fluorescent Probe, Zinquin: Relationship to Metallothionein and Intracellular Zinc. Biochem J. 1994;303:781-786.
5. P. D. Zalewski, et al., Flux of Intracellular Labile Zinc During Apoptosis (Gene-Directed Cell Death) Revealed by a Specific Chemical Probe, Zinquin. Chem Biol. 1994;1:153-161.
6. I. A. Brand, et al., Intercellular Zinc Movement and Its Effect on the Carbohydrate Metabolism of Isolated Rat Hepatocytes. J Biol Chem. 1996;271:1941-1949.
7. N. Wellinghausen, et al., Interaction of Zinc Ions with Human Peripheral Blood Mononuclear Cells. Cell Immunol. 1996;171:255-261.
8. P. D. Zalewski, et al., Changes in Distribution of Labile Zinc in Mouse Spermatozoa During Maturation in the Epidididymis Assessed by the Fluorophore Zinquin. Reprod Fertil Dev. 1996;8:1097-1105.
9. R. D. Palmiter, et al., ZnT-2, a Mammalian Protein That Confers Resistance to Zinc by Facilitating Vesicular Sequestration. EMBO J. 1996;15:1784-1791.
10. D. Berendji, et al., Nitric Oxide Mediates Intracytoplasmic and Intranuclear Zinc Release. FEBS Lett. 1997;405:37-41.
11. M. Tsuda, et al., Expression of Zinc Transporter Gene, ZnT-1, Is Induced After Transient Forebrain Ischemia in the Gerbil. J Neurosci. 1997;17:6678-6684.