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MQAE

Item # Unit Size
M024-10
50 mg

For Research Use Only Products

MSDS
Chemical Name: N-Ethoxycarbonylmethyl-6-methoxyquinolinium bromide
CAS: 124505-60-8


Appearance:
 Pale yellow powder
Purity: ≥95.0% (HPLC)
MW: 326.19, C14H16BrNO3

Storage Condition :
0-5ºC
Shipping Condition : ambient temperature

Product Description
MQAE with bromide ion as a counteranion has fluorescence excitation at 355 nm and emission at 460 nm. The fluorescent intensity of MQAE decreases in proportion to the chloride increase in cells. Chloride ion concentrations less than 50 mM may be monitored without being affected by pH changes. MQAE has high water solubility and membrane permeability.

Chemical Structure


General Protocol*
Reagents:
  • 5 mM MQAE/Krebs-HEPES buffer
  • Krebs-HEPES buffer (20 mM HEPES, 128 mM NaCl, 2.5 mM KCl, 2.7 mM CaCl2, 1 mM MgCl2, 16 mM glucose, pH 7.4)
Protocol:
1. Wash the cells with Krebs-HEPES buffer 3 times.
2. Add 5 mM Cl probe solution to the cells and incubate at 37ºC for 1 hour.
3. Wash the cells with Krebs-HEPES buffer 5 times.
4. Determine the fluorescence intensity using a fluorescence microscope coupled with an image analyzer.
* Cell staining conditions depend on cell type, so it is necessary to optimize the conditions for each experiment
References
1. A. S. Verkman, et al., Synthesis and Characterization of Improved Chloride-sensitive Fluorescent Indicators for Biological Applications. Anal Biochem. 1989;178:355-361.
2. M. Inoue, et al., An ATP-driven Cl- Pump Regulates Cl- Concentrations in Rathippocampal Neurons. Neurosci Lett. 1991;134:75-78.
3. T. Nakamura, et al., Direct Measurement of Chloride Concentration in Newt Olfactory Receptors with the Fluorescent Probe. Neurosci Lett. 1997;237:5-8.