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MitoPeDPP

Item # Unit Size
M466-10
1 set (3 x 5ug)

For Research Use Only Products

Application: Fluorescent Probe for Mitochondrial Research


・Cell membrane permeable probe

・Mitochondria targeted imaging is possible

・Desirable fluorescent properties (ex. 452nm / em. 470 nm) 

 MSDS
Chemical Name: 3-[4-(Perylenylphenylphosphino)phenoxy]propyltriphenylphosphonium iodide

Appearance: 
Purity: ≥80.0% (HPLC)
MW: 882.74, 
C53H41IOP
2

Storage Condition: 0-5ºC, protect from light
Shipping Condition:
ambient temperature


Product Description

MitoPeDPP is a cell-membrane-permeable probe, Perylene-based dye. It specifically localizes in mitochondria due to the triphenylphosphonium moiety introduced.

As the excitation and emission wavelength of MitoPeDPP are 452 nm and 470 nm, respectively, the probe can be applied for lipophilic peroxide imaging in living cells.

*This probe has been developed by Dr. Shioji et al. at Fukuoka University, Department of Chemistry




Lipophilic Peroxides Detection in Mitochondria (cell line used: HepG2 cell)


A: MitoPeDPP stained Mitochondria with t-BHP treatment

B: MitoRed stained Mitochondria

C: Merged Image (A/B)





Selectivity of MitoPeDPP as a Peroxides Probe

The MitoPeDPP is specifically-oxidized by t-BHP in mitochondria (A) but not with ROS and RNS (B).

 A: MitoPeDPP stained cells with t-BHP treatment (t-BHP) and without (control).

 B: MitoPeDPP stained cells with ROS or RNS exposure.

*As ROS generators, PMA (O2-・), NOC7(NO), and SIN-1(ONOO-) were used in the experiments.

*Abbreviations

t-BHP:  tert-butylhydroperoxide

PMA:  Phorbol Myristate Acetate

NOC7:  1-Hydroxy-2-oxo-3-(N-methyl-3-aminopropyl)-3-methyl-1-triazine

SIN-1:  3-(Morpholinyl)sydnonimine, hydrochloride