JAPAN USA CHINA


Price & Availability : Please Select your country

 

Cytotoxicity LDH Assay Kit-WST

Item # Unit Size
CK12-01
100 tests
CK12-05
500 tests
CK12-20
2000 tests

For Research Use Only Products

∼ Feature ∼

  1. Both homogeneous and non-homogeneous assay possible
  2. Longer Working Solution Stability (0-5 degree for 6 months)
  3. No need to freeze the Working Solution
  4. High Linearity

Increase your Cytotoxicity Data Reliability With Cytotoxicity LDH Assay Kit-WST

 MSDS

Contents of the Kit: 100 tests
Dye Mixture :1 tube Assay Buffer :11 ml, 1 tube
Lysis Buffer :1.1 ml, 1 tube Stop Solution :5.5 ml, 1 tube

Contents of the Kit: 500 tests
Dye Mixture :1 tube Assay Buffer :55 ml, 1 tube
Lysis Buffer :5.5 ml, 1 tube Stop Solution :27.5 ml, 1 tube

Contents of the Kit: 2000 tests
Dye Mixture :4 tube Assay Buffer :55 ml, 4 tube
Lysis Buffer :5.5 ml, 4 tube Stop Solution :27.5 ml, 4 tube

Storage Condition: 0-5°C
Shipping Condition: ambient temperature


Required Equipment and Materials
Microplate reader with 490 nm filter, CO2 incubator, 96-well tissue culture plate(flat bottomed), 20 μl, 100-200 μl multi-channel pipette


Product Description
Lactate dehydrogenase(LDH) is an enzyme that presents in almost cell types and it catalyzes the oxidation of lactate to pyruvate in the presence of co-enzyme NAD+. Once Cells are impaired by stress, injuries, chemicals, or intercellular signals, LDH is rapidly released from the cell membrane. Thus, the measurement of the amount of released LDH from cells is one of the major methods to assess the cell death. Since Dojindo's Cytotoxicity LDH Assay Kit-WST neither reflects the activity of living cells nor is harmful to cells, it allows the assay to perform in wells containing both viable and damaged cells(homogeneous assay). In addition, non-homogeneous assay that is performed by using the cell culture supernatant is also possible. The kit components consist of highly stable reagents, with longer stability for the reconstituted working solution eliminating constant preparation of the Working Solution. Cytotoxicity LDH Assay Kit-WST is designed for use with 96 well plates or multiwell plate formats for high-throughput screening.


Cell Proliferation assay and Cell Death assay
Cell viability and cytotoxicity assays are used for drug screening and cytotoxicity test of chemicals. Cell Counting Kit-8(CCK-8) is a sensitive colorimetric assay for determining the number of viable cells in cell proliferation and cytotoxicity assays. CCK-8 allows convenient assays using Dojindo's tetrazolium WST-8, which produces a water-soluble formazan dye upon bioreduction in the presence of an electron mediator, 1-Methoxy PMS. WST-8 is bioreduced by cellular dehydrogenases in living cells. On the other hand, Cytotoxicity LDH Assay Kit-WST evaluates the cytotoxic level by measuring the amount of LDH released from the damaged cell membrane. Accessing the level of cytotoxicity by combining both CCK-8 and Cytotoxicity LDH Assay Kit-WST allow researchers to evaluate the detailed cell health conditions.

Stability of Working Solution


Reconstituted Working Solution has higher stability than competitor's kit, eliminating the additional time to prepare the Working Solution for each assay.


Comparison of Working Solution Stability


Linearity


Dojindo's Cytotoxicity LDH Assay Kit-WST has higher sensitivity than competitor's kits


Cytotoxicity of mitomycin C using HeLa cells

Culture medium: MEM, 10% FBS

Incubation: 37C, 5% CO2, 48 hours

1) S. F. Jin, H. L. Ma, Z. L. Liu, S. T. Fu, C. P Zhang, Y. He, "XL413, a cell division cycle 7 kinase inhibitor enhanced the anti-fibrotic effect of pirfenidone on TGF-β1-stimulated C3H10T1/2 cells via Smad2/4.", Exp Cell Res., 2015, 2015113000019.
2) S. Watanabe, C. S. Moniaga, S. Nielsen, M. Hara-Chikuma, "Aquaporin-9 facilitates membrane transport of hydrogen peroxide in mammalian cells.Aquaporin-9 facilitates membrane transport of hydrogen peroxide in mammalian cells.", Biochem Biophys Res Commun ., 2016, 471, 191.
3) L. Wu, T. Oshima, J. Shan, H. Sei, T. Tomita, Y. Ohda, H. Fukui, J. Watari, H. Miwa , "PAR-2 activation enhances weak acid-induced ATP release through TRPV1 and ASIC sensitization in human esophageal epithelial cells.", Am J Physiol Gastrointest Liver Physiol ., 2015, 309, G695.

4)T. Fukami, A. Iida, K. Konishi, M. Nakajima , "Human arylacetamide deacetylase hydrolyzes ketoconazole to trigger hepatocellular toxicity", Biochem. Pharmacol.., 2016, doi:10.1016/j.jphs.2016.07.007.

5) M. Tanaka, M. Yoneyama, T. Shiba, T. Yamaguchi, K. Ogita, "Protease-activated receptor-1 negatively regulates proliferation of neural stem/progenitor cells derived from the hippocampal dentate gyrus of the adult mouse ", J Pharmacol Sci., 2016, doi:10.1016/j.jphs.2016.05.005.
6) S. Wakatsuki, A. Furuno, M. Ohshima, and T. Araki, "Oxidative stress-dependent phosphorylation activates ZNRF1 to induce neuronal/axonal degeneration", J Cell Biol., 2015, 211, (4), 881.