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-SulfoBiotics- SSP4

Item # Unit Size
1 mg

For Research Use Only Products

Storage Condition : -20℃, protect from light
Shipping Condition : Blue Ice
Purity : ≥90.0% (HPLC)
Chemical name: 3',6'-Di(O-thiosalicyl)fluorescein 
M.W.: 604.65

Product Description:

SSP4 (Sulfane Sulfur Probe 4) is a novel fluorescent probe to detect sulfane sulfurs selectively. SSP4 itself is non-fluorescent, but it emits strong green fluorescence when it reacts with sulfane sulfurs. Thus, SSP4 enables high sensitive fluorescence detection and imaging of sulfane sulfurs.


        Fig. 1 Reaction of SSP4 with sulfane sulfurs

Fluorescent property:


Chemical species of Intravital sulfane sulfur:


Selectivity of the SSP4:

Experimental example:

Detection of sulfane sulfurs in cells treated with a sulfane sulfur donor or sulfane sulfur quencher.

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  2. T. Ida, T. Sawa, H. Ihara. Y. Tsuchiya, Y. Watanabe, Y. Kumagai, M. Suematsu, H. Motohashi, S. Fujii, T, Matsunaga, M. Yamamoto, K. Ono, N. O. Devarie-Baez, M. Xian, J. M Fukuto, and T. Akaike, “Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling”, Proc Natl Acad Sci U S A., 2014, 111, 7606.3) 
  3. E. Marutani, M. Sakaguchi, W. Chen, K. Sasakura, J. Liu, M. Xian, K. Hanaoka, T. Nagano, and F. Ichinose, "Cytoprotective effects of hydrogen sulfide-releasing N-methyl-D-aspartate receptor antagonists mediated by intracellular sulfane sulfur", Med. Chem. Commun., 2014, 5, 1577.4) 
  4. M. Sakaguchi, E. Marutani, H-S. Shin, W. Chen, K. Hanaoka, M. Xian, and F. Ichinose, “Sodium Thiosulfate Attenuates Acute Lung Injury in Mice”, Anesthesiology. 2014,121, 1248
  5. Y. Kimura, Y. Toyofuku, S. Koike, N. Shibuya, N. Nagahara, D. Lefer, Y. Ogasawara, H. Kimura "Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain", Scientific Reports, Article number: 14774 (2015)
  6. N. Takahashi, F. Wei, S Watanabe, M. Hirayama, Y. Ohuchi, A. Fujimura, T. Kaitsuka, I. Ishii, T. Sawa, H. Nakayama, T. Akaike and K. Tomizawa, “Reactive sulfur species regulate tRNA methylthiolation and contribute to insulin secretion. Nucl. Acids Res. (2016) doi: 10.1093/nar/gkw745”
  7. Krause NC, Kutsche HS, Santangelo F, DeLeon ER, Dittrich NP, Olson KR, Althaus M. Hydrogen sulfide contributes to hypoxic inhibition of airway transepithelial sodium absorption. Am J Physiol Regul Integr Comp Physiol. 2016 Sep 1;311(3):R607-17