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Amine-reactive PES

Item # Unit Size
10 mg

For Research Use Only Products

∼ Features ∼
-Simple modification of various enzymes

Chemical Description: 1-[3-(Succinimidyloxycarbonyl)propoxy]-5-ethylphenazinium triflate
Purity(Absorbance): ≥90.0%
MW: 557.50, C23H22F3N3O8S

Storage Condition: -20℃
Shipping Condition: Ambient temperature

Product Description

Amine-reactive PES is a novel amino-reactive electron mediator. The mediator is able to be linked to enzymes such as glucose dehydrogenase by a covalent bond, and so is expected to be applied to next generation biosensors.

A New Generation of Electron Mediator

By modification of enzyme with Amine-reactive PES, the mediator which binds to the enzyme surface is able to transfer the electrons from reduced co-factor to the electrode. This sensing system may be useful for reducing mediator leakage from the electrode and replacement of expensive metal complex compounds.

Modification of enzymes using Amine-reactive PES is patent pending.

Electrochemical Measurement of Glucose

The concentration of glucose was measured by modifying enzyme using arPES and this sensing system has the potential to develop continuous monitoring for various target molecules such as glucose.

The Sensing System is Applicable to the Following Enzymes

- FAD-dependent glucose dehydrogenase (FAD-GDH)
- L-Lactate oxidase (Lox)
- Fructosyl amino acid oxidase (FAOx)
- Cholesterol oxidase (ChOx)
- Pyranose oxidase (PyOx)

Procedure for PES-modification

Easy procedure: Just mix enzyme and amine-reactive PES

When it is difficult to take out all the powder from the container,
please add the solvent into a container and dissolve it before its use.
1) M. Hatada, N. Loew, Y. Takahashi, J. Okuda-Shimazaki, W. Tsugawa, A. Mulchandani, K. Sode. " Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference." Bioelectrochemistry, 2018, 121, 185.
2) Stefano Ferri, Katsuhiro Kojima and Koji Sode, “Review of Glucose Oxidases and Glucose Dehydrogenases: A Bird’ s Eye View of Glucose Sensing Enzymes”. Journal of Diabetes Science and Technology, 2011, 5(5),1068.
3) Kentaro Hiraka, Katsuhiro Kojima, Wakako Tsugawa, Ryutaro Asano, Kazunori Ikebukuro and Koji Sode, "Rational engineering of Aerococcus viridans l-lactate oxidase for the mediator modification to achieve quasi-direct electron transfer type lactate sensor", Biosens Bioelectron., 2020, 151, 111974.
4)G. Kim, H. Cho, P. Nandhakumar, J. Park, K. Kim and H. Yang, "Wash-Free, Sandwich-Type Protein Detection Using Direct Electron Transfer and Catalytic Signal Amplification of Multiple Redox Labels", Anal. Chem., 2022, doi:10.1021/acs.analchem.1c04615.