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Amine-reactive PES

Item # Unit Size
A543-10
10 mg

For Research Use Only Products

Feature

Simple modification of various enzymes


Chemical Description: 1-[3-(Succinimidyloxycarbonyl)propoxy]-5-ethylphenazinium triflate
Purity (Absorbance): ≥90.0%
MW: 557.50, C23H22F3N3O8S

Storage Condition : -20℃
Shipping Condition: Ambient temperature

MSDS


Product Description

Amine-reactive PES is a novel amino-reactive electron mediator. The mediator can able to be linked to enzymes such as glucose dehydrogenase by a covalent bond, and so is expected to be applied to next generation biosensors.



A New Generation of Electron Mediator

By modification of enzyme with Amine-reactive PES, the mediator which binds to the enzyme surface is able to transfer the electrons from reduced co-factor to the electrode. This sensing system may be useful for reducing mediator leakage from the electrode and replacement of expensive metal complex compounds.


Modification of enzymes using Amine-reactive PES is patent pending.


Electrochemical Measurement of Glucose


The concentration of glucose was measured by modifying enzyme using arPES and this sensing system has the potential to develop continuous monitoring for various target molecules such as glucose.


The Sensing System is Applicable to the Following Enzymes

- FAD-dependent glucose dehydrogenase (FAD-GDH)
- L-Lactate oxidase (Lox)
- Fructosyl amino acid oxidase (FAOx)
- Cholesterol oxidase (ChOx)
- Pyranose oxidase (PyOx)



Procedure for PES-modification

Easy procedure: Just mix enzyme and amine-reactive PES



References

M. Hatada, N. Loew, Y. Takahashi, J. Okuda-Shimazaki, W. Tsugawa, A. Mulchandani, K. Sode. " Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference." Bioelectrochemistry, 2018, 121, 185.