Biofilms are found in various environments. They are aggregates composed of microorganisms and their metabolites, such as extracellular polysaccharides. In recent years, there has been an increasing need to evaluate the amount of biofilm formed on materials, as well as an increasing need for drugs that inhibit biofilm formation. The Biofilm TestPiece Assay Kit uses crystal violet staining to measure the difference in biofilm formation between different materials. By using the Dojindo-developed lid, “TestPiece Holder”, a test piece can be firmly fixed, and the biofilm will not peel off during washing or staining processes. As well, multiple samples can be assayed simultaneously.
Crystal Violet (CV) Staining
In 1982, Dr. Karsten Pedersen determined a correlation between the absorbance of crystal violet when staining biofilms and the dry weight of biofilm. The crystal violet staining method is still widely used for biofilm detection to this day.
Reference: K. Pedersen,”Method for Studying Microbial Biofilms in Flowing-Water Systems”, Appl. Environ. Microbiol., 1982, 43(1), https://www.ncbi.nlm.nih.gov/pmc/articles/PMC241775/
Usage of the TestPiece Holder
Feature 1 : Dramatically reduced time and effort for measurement
Conventional methods require laborious washing steps for each well. With Dojindo’s TestPiece Holder, well-washing is unnecessary, and multiple samples can be processed easily.
Feature 2: Less variable results than existing assays
In the conventional method, biofilm is formed by placing a test piece on the bottom of a petri dish or beaker. The biofilm tends to peel off during the washing process, which results in inconsistent measurement values.
In this kit, the test piece is fixed to the TestPiece Holder, which prevents biofilm from peeling off during operation and produces less variable results.
Experimental example: Comparison of biofilm formation among test pieces of various materials
Using Dojindo’s kit, we were able to quantify the amount of biofilm that Staphylococcus aureus had formed on test pieces made of various materials (polystyrene [PS], polypropylene [PP], fiber-reinforced plastic [FRP], stainless steel [SUS], float glass [GL], and silicon rubber [SLC]).
Caution: 24-well plate is not included in Biofilm TestPiece Assay Kit (code: B606)
This kit consists of only the TestPiece Holder and dye solution (Crystal Violet solution). It does not include a 24-well plate, which is required as a receiving plate for TestPiece Holder.
For details, please refer to “Recommended 24-well plate” below.
Falcon® 24-well Clear Flat Bottom TC-treated Multiwell Cell Culture Plate, with Lid, Sterile, 50/Case,
Product Number 353047
Falcon® is a registered trademark of Corning Incorporeted, Life Sciences.
A limited number of 24-well plates are available *†
24-well plate for Biofilm TestPiece Assay Kit (product code: B608)
The 24-well plate package (B608) contains 8 plates that are compatible with the TestPiece Holder attached to the Biofilm TestPiece Assay Kit (B606). This 24-well Plate package (B608) is sold in limited quantities.
*The 24-well plate (B608) and the recommended 24-well Plate are the same plate.
†The 24-well plate (B608) is not sold individually.
You can order 1 set of B608 when you order 1 set of Biofilm TestPiece Assay Kit (B606).
Alternative product catalog: Measuring biofilm formation using pins
We have a variety of pin plate assay kits to measure the amount of biofilm formation or to measure the metabolic activity of bacteria in biofilm. Please select the kit that best suits your purpose.
The Biofilm Formation Assay Kit (product code: B601) is a pin plate assay for biofilm formation and also uses crystal violet staining for measurement. It contains all necessary components in a single kit.