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α-Ketoglutarate Assay Kit-Fluorometric
Item #: K261 -

α-Ketoglutarate (α-KG) is an important intermediate of the TCA cycle. It is used as an indicator of enhanced uptake of glucose metabolites into the TCA cycle and of enhanced glutaminolysis, the metabolic pathway for supplying α-ketoglutarate using glutamine as a substrate. α-Ketoglutarate plays an essential role in the production of glutamate and γ-aminobutyric acid (GABA) and contributes to the scavenging of reactive oxygen species. Additionally, a recent cancer study suggested that...

ATP Assay Kit-Luminescence
Item #: A550 -

Adenosine triphosphate (ATP) is an energy source for living cells and is synthesized by both glycolysis and mitochondrial oxidative phosphorylation. Mitochondria generate 95% of cellular ATP, and mitochondrial dysfunction reduces ATP levels in cells. Therefore, the measurement of ATP levels has been established as a proxy for mitochondrial activity. Decreased ATP levels are associated with cancer, aging, neurodegenerative diseases, and mitochondrial diseases. Cancer cells rely on glycolysis, a...

Glucose Assay Kit-WST  G264, G264-05, G264-20, Glucose
Item #: G264 -

Principle This kit can detect glucose found within cell culture medium or intracellular glucose by measuring the absorbance of the colored WST formazan dye. The degree of absorbance depends on the amount of glucose present within the sample.This kit contains a glucose standard solution that can be used to create a standard curve. This allows for the quantitation of glucose levels present in the sample. --> Procedure Procedures are so easy that you simply incubate the plate after the addition...

Glucose Uptake Assay Kit-Green Glucose Uptake Assay Kit-Green
Item #: UP02 -

Nutrient metabolism is necessary for energy production in cells and regulates various cellular functions, including gene expression. Glucose is one of the key substrates for the generation of ATP and to sustain cellular homeostasis. Thus, glucose metabolism has been the subject of intense investigations. In cancer research, tumor cells enhance glucose uptake and consumption for their growth and proliferation. Therefore, elevated glucose uptake is a marker of tumors, and glucose transporters...

Glutamate Assay-Kit-WST   Glutamate,  Glutamate Assay-Kit-WST , measurement, detection, glutamate ratio, reduced, oxidized, antioxidant
Item #: G269 -

--> Product Description Glutamate is an important neurotransmitter that contributes to the biosynthesis of protein and glutathione. An excess of glutamate is considered to be a cause of neurodegenerative disorders, including Alzheimer's disease. A recent study showed that an iron-dependent cell death called “ferroptosis” is induced by inhibition of cystine/glutamate antiporter (xCT) responsible for cystine uptake and glutamate release. As such, cancer research which targets xCT has also been...

Glutamine Assay-Kit-WST  Glutamine,  Glutamine Assay-Kit-WST , measurement, detection, glutamine ratio, reduced, oxidized, antioxidant
Item #: G268 -

--> Product Description Glutamine, a source of α-ketoglutarate (one of the tricarboxylic acid (TCA) cycle intermediates), is an important substance used for energy production and the syntheses of nucleic acids and other amino acids. Glutaminolysis, the process by which glutamine as a substrate is converted to α-ketoglutarate, is upregulated in cancer cells. A study reports that glutaminolysis has largely contributed to scavenging of reactive oxygen species and reduction of oxidized...

Lactate Assay Kit-WST  L256, L256-10,Lactate
Item #: L256 -

Lactate Assay Kit-WST can quantify lactate (the lower limit: 0.02 mmol/l), one of the glycolysis metabolites. Because the Lactate Assay Kit-WST corresponds to 96 well micro-plates, it allows for multiple samples to be measured at once. Lactate quantification has been widely used as a marker for monitoring changes in intracellular metabolic pathway in many different areas of cancer research. In recent years, there have been an increasing number of reports on using lactate as an indicator of...

NAD/NADH Assay Kit-WST N509, N509-10,NAD/NADH
Item #: N509 -

Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NAD+ levels can be determined by subtracting NADH levels from independently measured total NAD+/NADH levels. Principle: NAD/NADH Assay Kit-WST enables quantification of the amount of total NAD+/NADH, NADH and NAD+ in cells and measurement of...

NADP/NADPH Assay Kit-WST NADP/NADPH Assay Kit-WST, N510
Item #: N510 -

Principle: NADP/NADPH Assay Kit-WST enables quantitation of the amount of total NADP+/NADPH, NADPH and NADP+ in cells and measurement of their ratio. Sensitive Measurements of NADP+ and NADPH Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADPH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NADP+ levels can be determined by subtracting NADPH...