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α-Ketoglutarate Assay Kit-Fluorometric
Item #: K261 -

α-Ketoglutarate (α-KG) is an important intermediate of the TCA cycle. It is used as an indicator of enhanced uptake of glucose metabolites into the TCA cycle and of enhanced glutaminolysis, the metabolic pathway for supplying α-ketoglutarate using glutamine as a substrate. α-Ketoglutarate plays an essential role in the production of glutamate and γ-aminobutyric acid (GABA) and contributes to the scavenging of reactive oxygen species. Additionally, a recent cancer study suggested that...

ADP/ATP Ratio Assay Kit-Luminescence
Item #: A552 -

Normally, when the intracellular ATP production decreases, the degradation product ADP will be resynthesized to ATP to maintain the intracellular ATP concentrations. However, when the metabolism of ATP production is disrupted, ATP cannot be resynthesized from ADP, but intracellular ATP is converted to ADP and the ADP/ATP ratio increases. The change in ADP/ATP ratio is not only related to apoptosis and autophagy, but also intracellular energy metabolism, and is used as one of the indicators of...

Amino Acid Uptake Assay Kit
Item #: UP04 -

Amino acids are essential for intracellular protein and nucleic acid synthesis, Especially for cancer cells which are proliferating continually. Since the supply of acetyl CoA from the glycolytic pathway is decreased in cancer cells, they have a furthermore huge demand for amino acids which are an important nutrient source for the TCA cycle. The research has shown that cancer cells increase the expression of the amino acid transporter LAT1 (L-type amino acid transporter 1) and take up a large...

ATP Assay Kit-Luminescence
Item #: A550 -

Adenosine triphosphate (ATP) is an energy source for living cells and is synthesized by both glycolysis and mitochondrial oxidative phosphorylation. Mitochondria generate 95% of cellular ATP, and mitochondrial dysfunction reduces ATP levels in cells. Therefore, the measurement of ATP levels has been established as a proxy for mitochondrial activity. Decreased ATP levels are associated with cancer, aging, neurodegenerative diseases, and mitochondrial diseases. Cancer cells rely on glycolysis, a...

Cystine Uptake Assay Kit
Item #: UP05 -

The cystine transporter xCT is a crucial transporter that maintains cellular redox balance by regulating glutathione synthesis via cystine uptake. xCT is highly expressed in several types of cancer cells and is expected to be a therapeutic target for cancer. Therefore, xCT has recently attracted researchers’ attention as one of the targets for cancer treatment. The xCT inhibitors sulfasalazine and erastin are known to reduce intracellular glutathione levels by inhibiting cystine uptake,...

Fatty Acid Uptake Assay Kit
Item #: UP07 -

This kit uses Fatty Acid Uptake Probe as a fatty acid analog, which can be uptaken into cells via fatty acid transporters on the cell membrane surface. The cells' fatty acids uptake ability can be evaluated by the fluorescence intensity with fluorescence microscopy, flow cytometers, plate readers, or other fluorescence instruments. In addition, a reagent that eliminates the fluorescence of any extracellular fatty acid analog is included, so it is possible to easily measure the actual fatty...

Glucose Assay Kit-WST  G264, G264-05, G264-20, Glucose
Item #: G264 -

Principle This kit can detect glucose found within cell culture medium or intracellular glucose by measuring the absorbance of the colored WST formazan dye. The degree of absorbance depends on the amount of glucose present within the sample.This kit contains a glucose standard solution that can be used to create a standard curve. This allows for the quantitation of glucose levels present in the sample. --> Procedure Procedures are so easy that you simply incubate the plate after the addition...

Glucose Uptake Assay Kit-Green Glucose Uptake Assay Kit-Green
Item #: UP02 -

Nutrient metabolism is necessary for energy production in cells and regulates various cellular functions, including gene expression. Glucose is one of the key substrates for the generation of ATP and to sustain cellular homeostasis. Thus, glucose metabolism has been the subject of intense investigations. In cancer research, tumor cells enhance glucose uptake and consumption for their growth and proliferation. Therefore, elevated glucose uptake is a marker of tumors, and glucose transporters...

Glutamate Assay-Kit-WST   Glutamate,  Glutamate Assay-Kit-WST , measurement, detection, glutamate ratio, reduced, oxidized, antioxidant
Item #: G269 -

--> Product Description Glutamate is an important neurotransmitter that contributes to the biosynthesis of protein and glutathione. An excess of glutamate is considered to be a cause of neurodegenerative disorders, including Alzheimer's disease. A recent study showed that an iron-dependent cell death called “ferroptosis” is induced by inhibition of cystine/glutamate antiporter (xCT) responsible for cystine uptake and glutamate release. As such, cancer research which targets xCT has also been...

Glutamine Assay-Kit-WST  Glutamine,  Glutamine Assay-Kit-WST , measurement, detection, glutamine ratio, reduced, oxidized, antioxidant
Item #: G268 -

--> Product Description Glutamine, a source of α-ketoglutarate (one of the tricarboxylic acid (TCA) cycle intermediates), is an important substance used for energy production and the syntheses of nucleic acids and other amino acids. Glutaminolysis, the process by which glutamine as a substrate is converted to α-ketoglutarate, is upregulated in cancer cells. A study reports that glutaminolysis has largely contributed to scavenging of reactive oxygen species and reduction of oxidized...

Glycolysis/OXPHOS Assay Kit
Item #: G270 -

Many cancer cells produce ATP through energy metabolism that depends on the glycolytic pathway. On the other hand, it has been recently reported that cancer cells whose glycolytic pathway is suppressed survive by shifting their energy metabolism to oxidative phosphorylation(OXPHOS). These phenomena has attracted much attention because it will not only help elucidate the mechanisms of anticancer drugs but also lead to the development of therapeutic strategies in various diseases including aging...

Lactate Assay Kit-WST  L256, L256-10,Lactate
Item #: L256 -

Lactate Assay Kit-WST can quantify lactate (the lower limit: 0.02 mmol/l), one of the glycolysis metabolites. Because the Lactate Assay Kit-WST corresponds to 96 well micro-plates, it allows for multiple samples to be measured at once. Lactate quantification has been widely used as a marker for monitoring changes in intracellular metabolic pathway in many different areas of cancer research. In recent years, there have been an increasing number of reports on using lactate as an indicator of...

NAD/NADH Assay Kit-WST N509, N509-10,NAD/NADH
Item #: N509 -

Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NAD+ levels can be determined by subtracting NADH levels from independently measured total NAD+/NADH levels. Principle: NAD/NADH Assay Kit-WST enables quantification of the amount of total NAD+/NADH, NADH and NAD+ in cells and measurement of...

NADP/NADPH Assay Kit-WST NADP/NADPH Assay Kit-WST, N510
Item #: N510 -

Principle: NADP/NADPH Assay Kit-WST enables quantitation of the amount of total NADP+/NADPH, NADPH and NADP+ in cells and measurement of their ratio. Sensitive Measurements of NADP+ and NADPH Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADPH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NADP+ levels can be determined by subtracting NADPH...