This kit can detect glucose found within cell culture medium or intracellular glucose by measuring the absorbance of the colored WST formazan dye. The degree of absorbance depends on the amount of glucose present within the sample.This kit contains a glucose standard solution that can be used to create a standard curve. This allows for the quantitation of glucose levels present in the sample.
Procedures are so easy that you simply incubate the plate after the addition of culture supernatant or tissue/cell lysates prior to adding a reagent.
Preparation of Standard Curve
Glucose levels in a sample can be measured by a calibration curve established with Glucose Standards included in this kit. If the glucose levels are greater than or equal to 0.5 mmol/L, the sample must be diluted before measurement.
Measurement of Glucose level in combination with Lactate Assay Kit
By using Glucose Assay Kit-WST and Lactate Assay Kit-WST, we have successfully measured metabolic activity changes of phloretin, a protein transport inhibitor, when it is added to Jurkat cells.
Precautions when using this kit.
Cell counts may need to be normalized. When cells are analyzed in a microplate, the results obtained may sometimes differ depending on cell numbers per well.In such cases, normalization of the measured values obtained from cell counting and total protein will be necessary.In this kit, cell numbers can be easily measured by the fluorescence intensity induced by a reagent added to cell culture medium for staining nuclei.
Storage Condition：Store at 0-5oC
Shipping Condition： ambient temperature
Ｑ： I do not have a 450 nm filter. What other filters can I use?
Ａ：You can use filters with an absorbance between 450 nm and 490 nm. However, the absorbance value can become lower if the sample is not measured at 450 nm.
Ｑ：Can I quantify L-Glucose levels?
Ａ：No, the kit is used for the quantification of β-D-Glucose levels.
Ｑ：How many samples can I measure?
* The number of samples that can be recorded when the standard curve and sample is measured in triplicates.
*A plate layout for Glucose standard solution and sample
1) M. Shinohara, Y. Tashiro, M. Shinohara, J. Hirokawa, K. Suzuki, M. O. Takeya, M. Mukouzono, S. Takeda, T. Saito, A. Fukumori, T. C. Saido, R. Morishita and N. Sato, "Increased levels of Aβ42 decrease the lifespan of ob/ob mice with dysregulation of microglia and astrocytes”
, FASEB J
2) K. Yamanaka, Y. Hamano and T. Oikawa, "Enhancement of metabolic flux toward ε-poly-l-lysine biosynthesis by targeted inactivation of concomitant polyene macrolide biosynthesis in Streptomyces albulus.”
, J. Biosci. Bioeng
3) K. Ohshima, S. Nojima, S. Tahara, M. Kurashige, K. Kawasaki, Y. Hori, M. Taniguchi, Y. Umakoshi, D. Okuzaki, N. Wada, J. Ikeda, E. Fukusaki and E. Morii, "Serine racemase enhances growth of colorectal cancer by producing pyruvate from serine”
, Nat Metab
, 2(1), 81.