The plasma membrane (PM), consists of a lipid bilayer separating the intracellular environment from the extracellular space. Consequently, the PM plays a central role in many cell behaviors, such as cell migration, cell stretching, and signaling cascades. Additionally, PM dysfunction is an important biomarker because it is related to the cell status and is linked to many diseases.
Dojindo’s PlasMem Bright dyes overcome these limitations. PlasMem Bright dyes are designed to stain PMs for over a day. Furthermore, the PlasMem Bright dyes are more water-soluble compared with other commercially available dyes and can be diluted with culture medium. The PlasMem Bright dyes offer two different color options (green and red) and are provided as ready-to-use DMSO solutions. A working solution can be prepared easily via a single dilution step using growth medium or HBSS.
The general number of usable assays per 100 ul
35 mm dish x 10
μ-Slide 8 well x 10
Low toxicity, No washing, and High retentivity
High retentivity on plasma membrane
HeLa cells stained with each plasma membrane staining reagent were incubated for 24 hrs and each the resulting fluorescent image was compared. PlasMem Bright series had higher retentivity on plasma membrane than other products.
Example of experiment: Plasma membrane staining inside an ES cell colony
Mouse ES cells were seeded in gelatin-coated glass bottom dishes and cultured for 4 days. The resulting colonies were stained with PlasMem Bright Green (200x dilution) for 15 minutes and observed the colonies under a confocal microscope (Zeiss: LSM710) after replacing medium.
As a result, the plasma membranes inside the colonies could be also clearly visualized with PlasMem Bright Green.
Ex. 488 nm / Em. 500 – 560 nm
*This data was kindly provided by Dr. Otsugu Ishizu, School of Medicine, Keio University.
Clear visualization of plasma membrane
Observe morphology of neuron (differentiated SH-SY5Y cells) and localization of mitochondria in axon.
Excitation and emission spectra of PlasMem Bright dyes
Nuclear (Blue), Mitochondria (Red), Plasma Membrane (Green)