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Item #: M470 -
Product Description 1-Methoxy PES is an electron mediator which has higher stability of solutions than 1-Methoxy PMS(M003). The stability in neutral to alkali conditions has been extremely improved with 1-Methoxy PES. 1-Methoxy PES is a stable small-molecular compound and it has an equal or higher thermal stability than diaphorase. The 1-Methoxy PES solution can be stored long term. Characteristics of Each Electron Mediator ...
![1-Methoxy PMS 1-Methoxy PMS, 1-Methoxy-5-methylphenazinium methylsulfate [CAS: 65162-13-2]](/resize/Shared/Images/Product/1-Methoxy-PMS/M003-new.png?bh=250)
Item #: M003 -
Reaction Scheme Product Description 1-Methoxy PMS is easily dissolved by water and alcohol. Its redox potential is +63 mV. Though phenazinium methylsulfate (PMS) is commonly used as an electron carrier for NADH-tetrazolium, the stability of PMS is very poor. However, 1-Methoxy PMS solution can be stored at room temperature for over 3 months without protection from light. An electron of NAD(P)H is transfered by 1-Methoxy PMS to tetrazolium salt (Reaction Scheme). Therefore, it is a useful...
![Amine-reactive PES Amine-reactive PES, 1-[3-(Succinimidyloxycarbonyl)propoxy]-5-ethylphenazinium triflate [CAS: ]](/resize/Shared/Images/Product/Amine-reactive-PES/A543-new.png?bh=250)
Item #: A543 -
Product Description Amine-reactive PES is a novel amino-reactive electron mediator. The mediator is able to be linked to enzymes such as glucose dehydrogenase by a covalent bond, and so is expected to be applied to next generation biosensors. A New Generation of Electron Mediator By modification of enzyme with Amine-reactive PES, the mediator which binds to the enzyme surface is able to transfer the electrons from reduced co-factor to the electrode. This sensing system may be useful for...
![DAB 3,3’-Diaminobenzidine, tetrahydrochloride [CAS: 7411-49-6]](/resize/Shared/Images/Product/DAB/D006-new.png?bh=250)
Item #: D006 -
Product Description DAB is one of the most commonly used oxidative chromogenic dyes for the detection of peroxidase in immunohistochemistry. In the presence of H2O2 and peroxidase, DAB is oxidized into a brown pigment. These brown pigments are aniline black type compounds and are firmly deposited around peroxidase on cell membranes or tissues. Most DAB available on the market appears brown as a result of oxidation during processing or storage. On the other hand, Dojindo offers a high quality...
![MTT MTT, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide [CAS: 2348-71-2]](/resize/Shared/Images/Product/MTT/M009-new.png?bh=250)
Item #: M009 -
Reaction Scheme Product DescriptionMTT is slightly soluble in methanol, but has very low solubility in ethanol. It barely dissolves in ether, acetone, or ethyl acetate. MTT is utilized for cell viability detection. Since MTT has a positive charge, it can pass through a cell membrane and is reduced by mitochondria to form a purple color formazan dye. Organic solvent is requried to dissovle MTT formazan dyes. ...
![Nitro-TB Nitro-TB, 3,3-[3,3’-Dimethoxy-(1,1’-biphenyl)-4,4’-diyl]-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride] [CAS: 298-83-9]](/resize/Shared/Images/Product/Nitro-TB/N011-new.png?bh=250)
Item #: N011 -
Reaction Scheme Product Description Nitro-TB is dissolved by hot water or hot methanol. Other organic solvents such as acetone and ether cannot solubilize Nitro-TB. Nitro-TB is easily reduced by dehydrogenase to purple formazan dye aggregates. It is used for dehydrogenase detection on agarose gels. ...
Item #: S302 -
Oxidation Reaction Product Description SAT-3 is a stable, highly water-soluble o-Tolidine analog. It is easily oxidized by peroxidase and hydrogen peroxide into a green dye at pH 4-6. While TMBZ requires an organic solvent or detergent to solubilize, SAT-3 can be solubilized with just buffer solution. The SAT-3 solution can be stored at room temperature with protection from light. The maximum wavelength of the dye is 675 nm. Using the Ames test, Nno mutagenicity of SAT-3 was detected. using...
![TB TB, 3,3-[3,3’-Dimethoxy-(1,1’-biphenyl)-4,4’-diyl]-bis(2,5-diphenyl-2H-tetrazolium chloride) [CAS: 1871-22-3]](/resize/Shared/Images/Product/TB/T012-new.png?bh=250)
Item #: T012 -
Reaction Scheme Product Description TB is slightly soluble in water, but does not dissolve easily in acetone, ethyl acetate, and ether. Other organic solvents such as methanol, ethanol, and tetrachloromethane can solubilize TB pretty well. Since TB is easily reduced with dehydrogenase to generate reddish-purple formazan dye aggregates, it is used for dehydrogenase detection in tissue samples. ...
![TMBZ TMBZ, 3,3’,5,5’-Tetramethylbenzidine [CAS: 54827-17-7]](/resize/Shared/Images/Product/TMBZ/T022-new.png?bh=250)
Item #: T022 -
Product Description TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine, which is a carcinogenic chemical. Because of the ortho methyl groups on its benzene ring, TMBZ is not metabolized into highly carcinogenic o-hydroxybenzidines or o,o E dihydroxybenzidines. Therefore, TMBZ compounds are much less carcinogenic than benzidine. Although the TMBZ solution is colorless, it turns bluish-green (λmax: 655 nm) in the presence of...
![TMBZ HCl TMBZ HCl, 3,3’,5,5’-Tetramethylbenzidine dihydrochloride, dihydrate [CAS: 64285-73-0]](/resize/Shared/Images/Product/TMBZ-HCl/T039-img.png?bh=250)
Item #: T039 -
Product Description TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine, which is a carcinogenic chemical. Because of the ortho methyl groups on its benzene ring, TMBZ is not metabolized into highly carcinogenic o-hydroxybenzidines or o,o E dihydroxybenzidines. Therefore, TMBZ compounds are much less carcinogenic than benzidine. Although the TMBZ solution is colorless, it turns bluish-green (λmax: 655 nm) in the presence of...
![WST-1 WST-1, 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt [CAS: 150849-52-8]](/resize/Shared/Images/Product/WST-1/W201-new.png?bh=250)
Item #: W201 -
Oxidation Reaction Product Description of WSTs Water-soluble tetrazolium salts (WSTs) were developed by introducing positive or negative charges and hydroxy groups to the phenyl ring of the tetrazolium salt. Positive charges, such as trialkylammonio groups, improve the water-solubility of the formazan dye. However, a large cation is easily precipitated out with organic anions such as carboxylate or phosphate. Although a hydroxy group also improved the water solubility of the tetrazolium salt,...
Item #: W202 -
Oxidation Reaction Product Description of WSTs Water-soluble tetrazolium salts (WSTs) were developed by introducing positive or negative charges and hydroxy groups to the phenyl ring of the tetrazolium salt. Positive charges, such as trialkylammonio groups, improve the water-solubility of the formazan dye. However, a large cation is easily precipitated out with organic anions such as carboxylate or phosphate. Though a hydroxy group also improved the water-solubility of the tetrazolium salt,...
![WST-4 WST-4, 2-Benzothiazolyl-3-(4-carboxy-2-methoxyphenyl)-5-[4-(2-sulfoethylcarbamoyl)phenyl]-2H-tetrazolium [CAS: 178925-54-7]](/resize/Shared/Images/Product/WST-4/W203-new.png?bh=250)
Item #: W203 -
Oxidation Reaction Product Description of WSTs Water-soluble tetrazolium salts (WSTs) were developed by introducing positive or negative charges and hydroxy groups to the phenyl ring of the tetrazolium salt. Positive charges, such as trialkylammonio groups, improve the water-solubility of the formazan dye. However, a large cation is easily precipitated out with organic anions such as carboxylate or phosphate. Though a hydroxy group also improved the water-solubility of the tetrazolium salt,...
![WST-5 WST-5, 2,2-Dibenzothiazolyl-5,5-bis[4-di(2-sulfoethyl)carbamoylphenyl]-3,3-(3,3-dimethoxy-4,4-biphenylene)ditetrazolium, disodium salt [CAS: 178925-55-8]](/resize/Shared/Images/Product/WST-5/W204-new.png?bh=250)
Item #: W204 -
Oxidation Reaction Product Description of WSTs Water-soluble tetrazolium salts (WSTs) were developed by introducing positive or negative charges and hydroxy groups to the phenyl ring of the tetrazolium salt. Positive charges, such as trialkylammonio groups, improve the water-solubility of the formazan dye. However, a large cation is easily precipitated out with organic anions such as carboxylate or phosphate. Though a hydroxy group also improved the water-solubility of the tetrazolium salt,...
Item #: W209 -
Product Description WST-8 formazan is water-soluble dye which is formed by reduction of WST-8 tetrazolium salt and had maximum absorbance at 460 nm Fig.1 Chemical Structure of WST-8 formazan <Caution> When it is difficult to take out all the powder from the container, please add the solvent into a container and dissolve it before its use. ...
![WST-9 WST-9, 2-(4-Nitrophenyl)-5-phenyl-3-[4-(4-sulfophenylazo)-2-sulfophenyl]-2H-tetrazolium, monosodium salt](/resize/Shared/Images/Product/WST-9/W217-new.png?bh=250)
Item #: W217 -
Oxidation Reaction Product Description of WSTs Water-soluble tetrazolium salts (WSTs) were developed by introducing positive or negative charges and hydroxy groups to the phenyl ring of the tetrazolium salt. Positive charges, such as trialkylammonio groups, improve the water-solubility of the formazan dye. However, a large cation is easily precipitated out with organic anions such as carboxylate or phosphate. Though a hydroxy group also improved the water-solubility of the tetrazolium salt,...