Transfection

Description:Various methods have been developed to express specific proteins in mammalian cells. The first method to introduce DNA to cells was calcium phosphateprecipitation. However, the transfection efficiency was very poor and there was a high rate of cell-to-cell variation. The second method introduced was the DEAEsephadex method. The transfection efficiency drastically improved, but still the method could not be used for all cells and required heavy metalions to enhance transfection...