Cell Counting Kit-8
~July 2018 Publications~


[HTML] S100A4 suppresses cancer stem cell proliferation via interaction with the IKK/NF-?B signaling pathway

Y Zhu, Y Zhou, X Zhou, Y Guo, D Huang, J Zhang… - BMC cancer, 2018

… at a density of 1 × 10 3 cells per well in a 96-well plate, followed by incubating
for 72 h. After incubation, cell counting kit-8 reagent (CCK-8, Dojindo Molecular
Technologies, Kumamoto, Japan) was added to each well with 10 µl at a …

[PDF] Downregulation of HOTTIP regulates insulin secretion and cell cycle in islet ß cells via inhibiting MEK/ERK pathway

X Xu, J Tian, QY LI - European Review for Medical and Pharmacological …, 2018

… well. To- tally, 100 µL of 10% Cell Counting Kit-8 (CCK-8) solution (Dojindo,
Kumamoto, Japan) was added to each well for 1 h incubation. The absorbance
at 450 nm of each sample was measured by a micro- plate reader …

[PDF] DUXAP10 regulates proliferation and apoptosis of chronic myeloid leukemia via PTEN pathway

R Yao, WT FENG, LJ XU, XM ZHONG, H Liu, Y Sun… - European Review for …, 2018

… CCK-8 (Cell Counting Kit-8) Assay Transfected cells were seeded into 96-well plates
with 1 × 104 per well. 10 µL of the CCK-8 solution (Dojindo, Kumamoto, Japan) was
added in each well. The absorbance at 450 nm of each …

Inhibitory effect of vanillin on RANKL-induced osteoclast formation and function through activating mitochondrial-dependent apoptosis signaling pathway

Y Chen, C Dou, J Yi, R Tang, T Yu, L Zhou, W Luo… - Life Sciences, 2018

… The cell proliferation and viability were evaluated by adding Cell Counting
Kit-8 (CCK8, Dojindo, Japan) reagent and the absorbance at 450 nm was
measured by using multi-detection microplate reader (BioTek Instruments …

AMPK-Mediated BECN1 Phosphorylation Promotes Ferroptosis by Directly Blocking System Xc–Activity

X Song, S Zhu, P Chen, W Hou, Q Wen, J Liu, Y Xie… - Current Biology, 2018

Skip to main content …

MicroRNA-449a suppresses hepatocellular carcinoma cell growth via G1 phase arrest and the HGF/c-Met pathway

J Cheng, LM Wu, XS Deng, J Wu, Z Lv, HF Zhao… - Hepatobiliary & Pancreatic …, 2018

… hours. Cell viability was measured using Cell Counting Kit-8 (Dojindo Laboratories,
Japan). The absorbance optical density was detected at 450 nm by using a microplate
reader (Elx800, BioTek, Vermont, USA). Invasion assay …

Molecular basis for RGD-containing peptides supporting adhesion and self-renewal of human pluripotent stem cells on synthetic surface

P Zhou, B Yin, R Zhang, Z Xu, Y Liu, Y Yan, X Zhang… - Colloids and Surfaces B …, 2018

… Y-27632 at 5 µM (Sigma-Aldrich, USA) was added only during passaging, and the medium
was changed daily. The cell number at each 24 h interval for 96 h was determined using
a cell counting kit-8 assay (CCK-8, Dojindo, Japan) as described previously [36] …

[PDF] Effects of curcumin on the role of MMP-2 in endometrial cancer cell proliferation and invasion

MX SUN, F Yu, ML GONG, GL FAN, CX LIU - European Review for Medical and …, 2018

… Instruments include: Cell Counting Kit-8 (CCK-8; Dojindo, Japan);
StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA,
USA); Western Blotting Analysis System (GE Healthcare Life Sciences …

Promotion of SIRT1 protein degradation and lower SIRT1 gene expression via reactive oxygen species is involved in Sb-induced apoptosis in BEAS-2b cells

X Zhao, Y Jin, L Yang, Z Hou, Y Liu, T Sun, J Pei, J Li… - Toxicology Letters, 2018

… 2.3. CCK8 analysis. The effects of various treatments on cell viability were determined
by Cell Counting Kit-8 (CCK-8) reagent (DOJINDO, CK04) as described previously
(Dong et al., 2016). BEAS-2b cells were plated in 96-well …

SIRT1 downregulation mediated Manganese-induced neuronal apoptosis through activation of FOXO3a-Bim/PUMA axis

X Zhao, Y Liu, G Zhu, Y Liang, B Liu, Y Wu, M Han… - Science of The Total …, 2018

… 2.4 Cell viability analysis The effects of various treatments on cell viability were determined
by Cell Counting Kit-8 (CCK-8) reagent (DOJINDO, CK04) as described previously (Zhao
et al., 2017). Briefly, PC12 cells were plated in 96-well plates at a density of 1 × 10 …

[PDF] ? hen the effect of APAP on osteoblastic differentiation and cell migration, with a particular focus on TRP

Y Nakatsu, F Nakagawa, S Higashi, T Ohsumi - Pharmacological Reports, 2018

… Then 10 pL of Cell Counting Kit-8 (Dojindo) was added to each well and the plate was
incubated at 37°C in a CO, incubator for 3 h, followed by measuring the absorbance at
450 nm using a microplate reader (BioRad, Hercules, CA, USA) …

Intracellular redox status controls spherogenicity, an in vitro cancer stem cell marker, in thyroid cancer cell lines

M Shimamura, K Yamamoto, T Kurashige… - Experimental Cell Research, 2018

… Fujifilm Wako Pure Chemical, Osaka, Japan), etoposide (Eto) (2.5 to 20
µM; Fujifilm Wako Pure Chemical) or irradiation (2 to 10 Gy) for 72 h. Cell
viability was then measured using a cell counting kit -8 (Dojindo, Kumamoto …

[HTML] An Engineered DC-Targeting Lentivector Induces Robust T Cell Responses and Inhibits HBV Replication in HBV Transgenic Mice via Upregulating T Cell Autophagy

S Ma, X Chen, Q Tan, S Dai, D Li, S Wu, Y Yu, G Zang… - Cellular Physiology and …, 2018

… a final volume of 200 µl in the presence of 1 µg concanavalin A (ConA, Sigma)
solution for 48 h. Cell proliferation was detected by incubating them with
Cell Counting Kit-8 (CCK-8) reagents (Dojindo, Shanghai, China) for 4 h …

[PDF] Upregulation of the Long Noncoding RNA SNHG3 Promotes Lung Adenocarcinoma Proliferation

L Liu, J Ni, X He - Disease Markers, 2018

… To ensure the quantitative accuracy, each sample was repeated 3 times.
2.4. Cell Proliferation Assay. We used Cell Counting Kit-8 (Dojindo Molecular
Technologies Inc., Japan) to perform cell proliferation analysis in octuplicate …

[PDF] MiR-138-5p is downregulated in patients with atrial fibrillation and reverses cardiac fibrotic remodeling via repressing CYP11B2

H Xie, JL FU, C Xie - European Review for Medical and Pharmacological …, 2018

Cell Counting Kit-8 Assay (CCK-8) A total of 4 × 103 cells were seeded in a 96- well
plate per well. All the wells were added with CCK-8 reagent (Dojindo, Tokyo, Japan)
respec- tively at 0, 24, 48, and 72 h according to the in- structions …

[HTML] Foxc2 and BMP2 Induce Osteogenic/Odontogenic Differentiation and Mineralization of Human Stem Cells from Apical Papilla

W Zhang, X Zhang, J Li, J Zheng, X Hu, M Xu, X Mao… - Stem Cells International, 2018

… with a-MEM medium supplied with 10% FBS. Cell Counting Kit 8 (CCK8, Dojindo,
Tokyo, Japan) was applied to analyze the proliferation of those cells on the 1st, 2nd,
4th, and 8th days after transfection. 2.4. RT-qPCR Analysis …

Prognostic Value of Trefoil Factor 3 Expression in Patients with Gastric Cancer

Y Taniguchi, Y Kurokawa, T Takahashi, J Mikami… - World Journal of Surgery, 2018

… transfected with lentivirus-based shRNA were seeded 3 × 10 3 cells per
well in 96-well plates and incubated under an atmosphere of 5% CO 2 at
37 °C. Cell proliferation was assessed 0, 72, and 144 h after seeding cells …

[PDF] Ipriflavone promotes osteogenesis of MSCs derived from osteoporotic rats

AG GAO, YC ZHOU, ZJ HU, BB LU - European Review for Medical and …, 2018

… CCK-8 (Cell Counting Kit-8) Assay MSCs treated with different doses of Iprifla- vone were
seeded in the 96-well plates at a density of 1.0×104 per well. After culturing for 24 h, 10
µL of CCK-8 solution (Dojindo, Kumamoto, Japan) were added into each well …

SNORA42 enhances prostate cancer cell viability, migration and EMT and is correlated with prostate cancer poor prognosis

C Yi, X Wan, Y Zhang, F Fu, C Zhao, R Qin, H Wu, Y Li… - The International Journal of …, 2018

… 2.7. Cell proliferation assay. Cell proliferation was assessed by Cell
Counting Kit-8 (CCK-8, Dojindo Laboratories, Japan) in octuplicate
according to the manufacturer's instructions. Absorbance was measured …

MiR-92a promotes tumorigenesis of colorectal cancer, a transcriptomic and functional based study

E Chen, Q Li, H Wang, F Yang, L Min, J Yang - Biomedicine & Pharmacotherapy, 2018

… 2.6. Cell proliferation assay. For proliferation assay, Cell Counting Kit-8
(Dojindo Laboratories, Japan) was used according to the manufacturer's
instructions. After 24 h of transfection, cells were inoculated at a density …

Effect of dual mTOR inhibitor on TGFß1-induced fibrosis in primary human urethral scar fibroblasts

S Huang, C Yang, M Li, B Wang, H Chen, D Fu… - Biomedicine & …, 2018

… 2.4. Cell proliferation. HUSFs were seeded in a 96-well plate at the density
of 5 × 10 3 cells per well in 90 µL volume, and Cell Counting Kit-8 (CCK8)
reagents (Dojindo, Kumamoto, Japan) were used according to the …

LncRNA FOXD2-AS1 regulates chondrocyte proliferation in osteoarthritis by acting as a sponge of miR-206 to modulate CCND1 expression

L Cao, Y Wang, Q Wang, J Huang - Biomedicine & Pharmacotherapy, 2018

… 2.4. Cell viability assay. Cells proliferation was determined by the Cell Counting
Kit-8 (CCK-8, Dojindo) according to the manufacturer's instructions. Briefly, a total
of approximate 2000 cells were seeded in the 96-well plates …

Effects of aligned and random fibers with different diameter on cell behaviors

X Li, X Wang, D Yao, J Jiang, X Guo, Y Gao, Q Li… - Colloids and Surfaces B …, 2018

… CCK8 Cell Counting Kit-8. BI Biological Industries. kV Kilovolt. cm Centimeter.
PBS Phosphate Buffer Saline. UV Ultraviolet. EDTA Ethylenediaminetetraacetic
Acid … CCK8 assay was purchased from Japanese Dojindo. Live/Dead Viability …

Expression Profile of LGR5 and Its Prognostic Significance in Colorectal Cancer Progression

BG Jang, HS Kim, WY Chang, JM Bae, WH Kim… - The American Journal of …, 2018

… 10 3 cells /well on 96-well plates and incubated at 37 °C. After adding 10µL
of Cell Counting Kit-8 reagent (Dojindo, Kumamoto, Japan) into each well
and incubating for 1 hour, absorbance was measured at 450nm using a …

Simple In Vivo Gene Editing via Direct Self-Assembly of Cas9 Ribonucleoprotein Complexes for Cancer Treatment

SM Kim, SC Shin, EEK Kim, SH Kim, K Park, SJ Oh… - ACS nano, 2018

… (e) The viability of three cells after treatment with various doses of ternary Cas9 RNPs
was analyzed by cell-counting kit-8 (CCK-8) assay. On-targeted (upper) and off-targeted
(lower) ternary Cas9 RNPs were incubated for 72 h. *P <0.05 , **P Page 10 of 26 …

Long noncoding RNA SNHG6 regulates p21 expression via activation of the JNK pathway and regulation of EZH2 in gastric cancer cells

Y Li, D Li, M Zhao, S Huang, Q Zhang, H Lin, W Wang… - Life Sciences, 2018

… 2.5. Cell proliferation and colony formation assay. Cell proliferation was measured using
the Cell Counting Kit-8 (CCK8, Dojindo, Japan). Cells were seeded in 96-well plates
(n = 6), with each well containing 2000 cells in 100 µL of cell suspension …

Long non-coding RNA HOTAIR promotes LPS-induced inflammatory injury by down-regulation of microRNA-124 in murine chondrogenic ATDC5 cells

G Duan, S Song, S Niu - Life Sciences, 2018

Kit-8 (CCK-8) assay. ATDC5 cells were seeded in 96-well plate with 5000
cells/well after treated with LPS and/or transfected with pc-HOTAIR,
sh-HOTAIR, miR-124 mimic and their corresponding negative controls …

Calcium phosphate particles stimulate exosome secretion from phagocytes for the enhancement of drug delivery

YJ Shyong, KC Chang, FH Lin - Colloids and Surfaces B: Biointerfaces, 2018

… After 24 h, CaP particles were added to the culture medium at a final concentration of
250, 500, 1000, and 2000 µg/ml, and the cells cultured for a further 1, 2, or 3 d. Cell viability
was then assayed using a Cell Counting Kit-8 (Dojindo, Kumamoto, Japan). 2.4 …

Long non-coding RNA PVT1 promotes malignancy in human endometrial carcinoma cells through negative regulation of miR-195-5p

F Kong, J Ma, H Yang, D Yang, C Wang, X Ma - Biochimica et Biophysica Acta (BBA) …, 2018

… 2.8. Cell proliferation assay. Cell proliferation assays were performed using the Cell
Counting Kit-8 (CCK8, Dojindo Molecular Technologies, Kumamoto, Japan) according
to the manufacturer's instructions. Cells were seeded …

Magnetic Field-inducible Drug-eluting Nanoparticles for Image-Guided Thermo-Chemotherapy, Magnetic field-inducible drug-eluting nanoparticles for image-guided …

GK Thirunavukkarasu, K Cherukula, H Lee, YY Jeong… - Biomaterials, 2018

… Waltham, MA, USA). Paraformaldehyde was obtained from DANA (Seoul, South
Korea). WST assay Cell Counting Kit-8 was purchased from Dojindo Laboratories
(Kumamoto, Japan). Reagents for immunohistochemistry were …

[HTML] Function of SLC7A7 in T-Cell Acute Lymphoblastic Leukemia

X Ji, X Yang, N Wang, M Kang, Y Wang, L Rong… - Cellular Physiology and …, 2018

… The ß-actin antibody was from Santa Cruz Biotechnology, Inc. (Dallas,
TX). Cell proliferation was measured using a Cell Counting Kit-8 (Dojindo,
Kumamoto, Japan). A cell cycle detection kit (Keygen Biotech, Nanjing …

[HTML] Uptake and transport of pullulan acetate nanoparticles in the BeWo b30 placental barrier cell model

H Tang, Z Jiang, H He, X Li, H Hu, N Zhang, Y Dai… - International Journal of …, 2018

… (Houston, TX, USA). Cell Counting Kit-8 was purchased from Dojindo Laboratories
(Kumamoto, Japan). The choriocarcinoma cell line BeWo b30 was donated by Professor
Erik Rytting of University of Texas Medical Branch (Galveston, TX, USA) …

[PDF] LncRNA ATB promotes the proliferation and metastasis of lung cancer via activation of the p38 signaling pathway

L Wei, T Wu, P He, JL Zhang, W Wu - Oncology Letters, 2018

… suspension. A total of 10 µl Cell Counting kit-8 (Dojindo Molecular Technologies, Inc.,
Kumamoto, Japan) in 100 µl RPMI-1640 medium was added to each well, and cultured
for 2 h at 37°C. The absorbance was measured at 450 nm …

[PDF] Reversing effect of NOTCH1 inhibitor LY3039478 on drug-resistance cells SGC7901/DDP of human gastric cancer and its mechanism

YL Wang, Y Zhang, XY Huang, Y Zheng - European Review for Medical and …, 2018

… Selective NOTCH1 inhibitor LY3039478 was purchased from Selleck (Houston, TX, USA),
Cell Counting Kit-8 (CCK-8) from Dojindo (Kumamoto, Japan), Hes protein antibody from
Cell Signaling Tech- nology (Danvers, MA, USA), and flow cytometer from Beckman Coulter …

[PDF] HOTTIP participates in mammary cancer by promoting cell proliferation via PI3K/AKT pathway

W Gao, XL WU, DZ LI, HD LIU - European Review for Medical and Pharmacological …, 2018

Cell Counting Kit-8 (CCK-8) Assay The cells were seeded in 96-well plates
at a density of 4×103/well and incubated in a constant temperature incubator …
After cell were cultured for 0 h, 24 h, 48 h, and 72 h, respectively, 10 µL of …

[PDF] CREB1, a direct target of miR-122, promotes cell proliferation and invasion in bladder cancer

L Guo, M Yin, Y Wang - Oncology Letters, 2018

… Signal detection employed ECL detection system (Thermo Fisher Scientific,
Inc., Waltham, MA, USA). Cell proliferation assay. The cell proliferative ability
was measured by Cell Counting Kit-8 assay (CCK-8; Dojindo Molecular …

[HTML] Antitumor Effects of DC Vaccine With ALA-PDT-Induced Immunogenic Apoptotic Cells for Skin Squamous Cell Carcinoma in Mice

H Zhang, P Wang, X Wang, L Shi, Z Fan, G Zhang… - Technology in Cancer …, 2018

Targeted immunotherapy using dendritic cell vaccine has been employed for the treatment
of solid tumors. Topical 5-aminolevulinic acid-mediated photodynamic the...

Effects of SOX15 on the colorectal cancer cells via downregulation of the Wnt/ß-catenin signaling pathway

S Wang, H Yang, X Chen, Z Jiang - Future Oncology, 2018

… Meanwhile, the mRNA and protein expression levels of the transfected
cells were detected by RT-PCR and western blot. Cell proliferation colony
formation assay. Cell counting kit-8 (Dojindo Molecular Technologies …

Insulin-like growth factor binding protein 4 inhibits proliferation of bone marrow mesenchymal stem cells and enhances growth of neurospheres derived from the stem …

H Li, S Yu, F Hao, X Sun, J Zhao, Q Xu, D Duan - Cell Biochemistry and Function, 2018

… 2 LI ET AL. Page 3. 2.3 | Proliferation of BMSCs The proliferation of BMSCs was
analysed using Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Kumamoto, Japan).
The P4 BMSC suspensions (3 × 104/mL) were inoculated …

[HTML] Interleukin-15 facilitates muscle regeneration through modulation of fibro/adipogenic progenitors

X Kang, M Yang, Y Shi, M Xie, M Zhu, X Zheng… - Cell Communication and …, 2018

… CCK-8 assay. For CCK-8 (cell counting kit 8) test, 1 × 10 4 FAPs were
stimulated by recombinant mouse IL-15 (100 ng/ml) or combined with
SAR-20347 (10 µM) for 48 h in 96-well plates. Then 10 µL CCK-8 solution …

Long non-coding RNA EPIC1 promotes human lung cancer cell growth

B Zhang, HY Lu, YH Xia, AG Jiang, YX Lv - Biochemical and Biophysical Research …, 2018

… Cell viability was tested by a Cell Counting Kit-8 (CCK-8, Dojindo, Japan) assay kit
according to the manufacturer's instructions. CCK-8 optical density (OD) of each well was
recorded at a wavelength of 550 nm. 2.8. EdU assay of cell proliferation …

[PDF] MiR-6836-3p promotes proliferation of hypertrophic scar fibroblasts by targeting CTGF

F Liu, WW CHEN, Y Li, JQ ZHANG, QB ZHENG - European Review for Medical and …, 2018

… in each well. 10 µL cell counting kit-8 (CCK8, Dojindo, Kumamoto, Japan) solution
mixed with 90 µL DMEM was added ito each well, and then, the plate was incu-
bated at 37°C for 2 h. Absorbance was measured at 450 nm …

Suppression of ferroportin expression by cadmium stimulates proliferation, EMT, and migration in triple-negative breast cancer cells

Z Shan, Z Wei, ZA Shaikh - Toxicology and Applied Pharmacology, 2018

… Transwell inserts were from EMD Millipore (Billerica, MA). Cell Counting Kit-8 was
obtained from Dojindo Molecular Technologies (Rockville, MD). ICP-MS Calibration
Standard was obtained from Ultra Scientific (North Kingstown, RI) …

Bufalin suppresses the proliferation and metastasis of renal cell carcinoma by inhibiting the PI3K/Akt/mTOR signaling pathway

J Xie, W Lin, L Huang, N Xu, A Xu, B Chen… - Oncology Letters, 2018

… WL01607), and PI3K (1:700 dilution, WL01169) were obtained from Wanlei Biological
Technology Co. (Shenyang, China). Cell viability assay. ACHN cell viability was detected
using the Cell Counting Kit-8 assay (CCK-8; Dojindo Laboratories, Kumamoto, Japan) …

Decreased expression of protein tyrosine kinase 6 contributes to tumor progression and metastasis in laryngeal squamous cell carcinoma

D Wu, X Zhang, Z Liu, H Yan, J Mai, Z Zhao, Q Zhong… - … and Biophysical Research …, 2018

… Cell viability assay was performed by Cell Counting Kit 8 (CCK8) assay using CCK8
assay kit (Dojindo, Japan) and by 5-Bromo-2-deoxyUridine (BrdU) assay using BrdU
detection antibody (Millipore, Germany). 2.7. Transwell assay …

Ureaplasma spp. lipid-associated membrane proteins Induce Human Monocyte U937 Cell Cycle Arrest through p53-independent p21 pathway

X Huang, Y Qiao, Y Zhou, Z Ruan, Y Kong, G Li, X Xie… - International Journal of …, 2018

… 2.4. Cell-Counting Kit-8 colorimetric assay. The U937 cells treated with LAMPs were
assessed with Cell-Counting Kit-8 (CCK8) colorimetric assay (CK04, Dojindo
Laboratories, Japan). Briefly, cells were incubated in a 96 well …

[PDF] LncRNA-CARl in a rat model of myocardial infarction

L Li, JJ WANG, HS ZHANG - European Review for Medical and Pharmacological …, 2018

… Biotechnology Co. Ltd. (Beijing, China); Cell counting kit-8 (CCK-8) was obtai- ned
from Dojindo (Kumamoto, Japan); Caspase 3 activity assay kit was obtained from
Shanghai Beyotime Biological Engineering Co. Ltd. (Shan …

[HTML] Neuroprotective effects of leonurine against oxygen–glucose deprivation by targeting Cx36/CaMKII in PC12 cells

J Li, S Zhang, X Liu, D Han, J Xu, Y Ma - PLOS ONE, 2018

… Cell viability was quantified using cell counting kit-8 (Dojindo, Japan). The PC12
cells were seeded at 100 µL/well in a 96-well plate according to the manufacturer's
instructions. After OGD induction, 10 µL of CCK-8 solution …

MiR-32 promotes tumorigenesis of colorectal cancer by targeting BMP5

E Chen, Q Li, H Wang, P Zhang, X Zhao, F Yang… - Biomedicine & …, 2018

… 2.6. Cell proliferation. Cell Counting Kit-8 (Dojindo Laboratories, Japan)
was used to detect cell proliferation activity according to the manufacturer's
instructions. After 48 h of transfection, cells were inoculated at a density …

[HTML] Preparation of porous calcium phosphate microspheres with phosphate-containing molecules at room temperature for drug delivery and osteogenic differentiation

JF Liu, L Wei, D Duolikun, XD Hou, F Chen, JJ Liu… - RSC Advances, 2018

… Technology Co., Ltd. The cell counting kit-8 (CCK8) was purchased from Dojindo, Japan.
All chemicals were of analytical grade and used directly without being further purified.
Preparation of the CaP microspheres. Typically, the CaP …

Alkyl-glycerophosphate-mediated CC motif chemokine 2 secretion induces oxidative stress via increased PPAR? activation in human umbilical vein endothelial cells

T Tsukahara, S Yamagishi, Y Matsuda, H Haniu - Biomedicine & Pharmacotherapy, 2018

… 4.3. Measurement of cell proliferation. HUVECs were seeded in 96-well
culture plates (5 × 10 3 cells/well), and AGP with or without an T0070907
or cPA was added to cells. After 48 h, cell proliferation was determined using …

[PDF] Long non-coding RNA NNT-AS 1 sponges miR-424/E2F1 to promote the tumorigenesis and cell cycle progression of gastric cancer

B Chen, Q Zhao, L Guan, H Lv, L Bie, J Huang… - Journal of Cellular and …, 2018

… 2.4 | Cell Counting Kit-8 assay Cell proliferation assay was performed with Cell Counting
Kit-8 (CCK-8; Dojindo Laboratories, Kumamoto, Japan). GC cells (MGC- 803, SGC-7901)
were seeded into 96-well plates at a density of 1 9 103 per well …

Inhibition of SETD7 protects cardiomyocytes against hypoxia/reoxygenation-induced injury through regulating Keap1/Nrf2 signaling

Y Dang, X Ma, Y Li, Q Hao, Y Xie, Q Zhang, F Zhang… - Biomedicine & …, 2018

… Cell viability was determined using cell counting kit-8 (CCK-8) assay. In
brief, cells were seeded at 1 × 10 4 cells/well in 96-well plates. After the
indicated treatments, cells were treated with CCK-8 reagent (Dojindo

Upregulation of circular RNA hsa_circ_0007534 predicts unfavorable prognosis for NSCLC and exerts oncogenic properties in vitro and in vivo

Y Qi, B Zhang, J Wang, M Yao - Gene, 2018

… Cell viability was then evaluated by Cell Counting Kit-8 (CCK-8, Dojindo, Japan) reagent
at day 0, 1, 2, 3, and 4. Each well was quantified by a microplate reader (Tecan, Männedorf,
Switzerland) at 450 nm after incubated at 37 °C for 2 h. 2.6. Clone formation assay …

Anti-proliferative and anti-invasive effects of garcinol from Garcinia indica on gallbladder carcinoma cells

YT Duan, XA Yang, LY Fang, JH Wang, Q Liu - Die Pharmazie-An International …, 2018

… the proliferation of GBC cells The proliferation inhibition effect of garcinol
on GBC cells (GBC-SD and NOZ) was determined using Cell Counting
Kit-8 (CCK-8 … 4.2. Cell viability assay The effect of garcinol on cell viability …

Oxymatrine induces cell cycle arrest and apoptosis and suppresses the invasion of human glioblastoma cells through the EGFR/PI3K/Akt/mTOR signaling pathway …

Z Dai, L Wang, X Wang, B Zhao, W Zhao, SS Bhardwaj… - Oncology reports, 2018

… Cell viability assay. A Cell Counting Kit-8 (CCK-8; Dojindo Molecular Technologies,
Inc., Kumamoto, Japan) assay was performed to explore the cytotoxic effects of
OM and/or erlotinib on human glioblastoma cells. U251MG …

Effects of Naringin on the Proliferation, Migration and Osteogenesis of Two Human Osteoblast-Like Cell Lines

LH Guo, Y Cao, RT Zhuang, LX Wang, Y Han, J Li - Journal of Biomaterials and …, 2018

… 2.3. Cell Counting Kit-8 Assay Cell proliferation was measured with Cell Counting
Kit-8 (CCK-8, Dojindo, Shanghai, China) assays. Cells were plated in 96-well plates at
a density of 10,000 cells per well in 100 l of complete medium and grown overnight …

MicroRNA-298 represses hepatocellular carcinoma progression by inhibiting CTNND1-mediated Wnt/ß-catenin signaling

N Cao, L Mu, W Yang, L Liu, L Liang, H Zhang - Biomedicine & Pharmacotherapy, 2018

… 2.6. Cell counting kit-8 (CCK-8) assay. HCC cells were added to a 96-well plate at a
density of 1 × 10 4 /mL. A total of 10 µL CCK-8 solution (Dojindo, Tokyo, Honshu, Japan)
was added to each well at the same time every day for 3 days …

Chlorin e6-embedded starch nanogels for improved photodynamic tumor ablation

SA Lim, H Park, JM Lee, ES Lee - Polymers for Advanced Technologies, 2018

… (Korea). Wheat Germ Agglu- tinin Alexa Fluor® 488 conjugate (WGA-Alexa Fluor®
488) was bought from LifeTechnologies (USA). Cell Counting Kit-8 (CCK-8) was
obtained from Dojindo Molecular Technologies Inc. (Japan) …

[PDF] SKP2 Promotes Hepatocellular Carcinoma Progression Through Nuclear AMPK-SKP2-CARM1 Signaling Transcriptionally Regulating Nutrient-Deprived Autophagy …

X Wei, X Li, W Yan, X Zhang, Y Sun, F Zhang - Cellular Physiology and Biochemistry, 2018

Cell Counting Kit-8 assays and colony formation assays. Cell viability was assessed
using the Cell counting Kit-8 assay (Dojindo Molecular Technologies, Inc., Shanghai,
China). Cells were seeded in 96-well plates at a density …

The In Vitro Impact of Glycyrrhizic Acid on CD4+ T Lymphocytes through OX40 Receptor in the Patients with Allergic Rhinitis

S Fouladi, M Masjedi, R Ghasemi, MG Hakemi… - Inflammation

… Bioidea, Tehran, Iran), which contained 10% fetal bovine serum and 1%
penicillin–streptomycin (Bioidea, Iran) under standard culture conditions
(37 °C; humid air (95%) and CO 2 (5%)). To perform the cell proliferation …

[HTML] Danggui Sini Decoction Protected Islet Endothelial Cell Survival from Hypoxic Damage via PI3K/Akt/eNOS Pathway

W Chen, C Huang, C Yang, X Ge, W Huang, X Li… - … -Based Complementary and …, 2018

Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Laboratories
(Kumamoto, Japan). In Situ Cell Death Detection Kit for TUNEL assay was
obtained from Roche (Basel, Switzerland). Cell-Light™ EdU Apollo488 In …

[HTML] Oxidative degradation of polyamines by serum supplement causes cytotoxicity on cultured cells

L Wang, Y Liu, C Qi, L Shen, J Wang, X Liu, N Zhang… - Scientific Reports, 2018

Article.

[PDF] Aldolase A as a prognostic factor and mediator of progression via inducing epithelial–mesenchymal transition in gastric cancer

Z Jiang, X Wang, J Li, H Yang, X Lin - Journal of Cellular and Molecular Medicine, 2018

… The cells were seeded at a density of 2000 cells/well in 96-well plates and incubated.
An aliquot of 10 lL of Cell Counting Kit-8 solution (CCK-8; Dojindo, Kumamoto, Japan)
was added to the wells and incubated for 2hours. The absorbance was measured at …

Maclurin exerts anti-cancer effects on PC3 human prostate cancer cells via activation of p38 and inhibitions of JNK, FAK, AKT, and c-Myc signaling pathways

YJ Lee, O Jung, J Lee, J Son, JY Cho, C Ryou, SY Lee - Nutrition Research, 2018

… CCK-8 assay kit was from Dojindo, Trizol solution was purchased from
Life Technologies Corporation (Carlsbad, USA). 2.2. Cell culture … 2.3.
Analysis of cell viability. We performed cell viability assay to measure cell …

[HTML] Radiobrominated benzimidazole-quinoline derivatives as Platelet-derived growth factor receptor beta (PDGFRß) imaging probes

N Effendi, K Mishiro, T Takarada, A Makino, D Yamada… - Scientific Reports, 2018

Article.

Effects of Transplanted Islets Nano-Encapsulated with Hyperbranched Polyethylene Glycol and Heparin on Microenvironment Reconstruction and Glucose Control

MR Haque, JH Jeong, KW Lee, GS Kim, SJ Kim… - Bioconjugate Chemistry, 2018

… microscopic images of FITC-labeled hb-PEG/Hep (SH-6-arm-PEG-Lipid,
1%; 6-arm-PEG-catechol, 2%; FITC-Heparin-SH, 2%). Viability checked for
hb-PEG/Hep islets in contrast to naked (control) islets by (b) live/dead …

[HTML] Cisplatin-activated PAI-1 secretion in the cancer-associated fibroblasts with paracrine effects promoting esophageal squamous cell carcinoma progression and …

Y Che, J Wang, Y Li, Z Lu, J Huang, S Sun, S Mao… - Cell Death & Disease, 2018

… from Gibco (New York, NY, USA). The Cell Counting Kit-8 (CCK-8) reagent was
purchased from Dojindo (Kumamoto, Japan). Crystal violet and ROS were purchased
from Beyotime (China). All the antibodies (cleaved caspase …

[HTML] Protein phosphatase 2A activation mechanism contributes to JS-K induced caspase-dependent apoptosis in human hepatocellular carcinoma cells

L Liu, Z Huang, J Chen, J Wang, S Wang - Journal of Experimental & Clinical Cancer …, 2018

… Reagents used in the present study included cell counting kit-8(CCK-8)
(Dojindo Laboratories, Kumamoto, Japan), Annexin V-FITC/PI kit (BD
Biosciences, NJ, San Diego, CA, USA), DAPI staining solution, 3-Amino …

In vivo and in situ activated aggregation-induced emission probes for sensitive tumor imaging using tetraphenylethene-functionalized trimethincyanines-encapsulated …

X Zhang, B Wang, Y Xia, S Zhao, Z Tian, P Ning… - ACS applied materials & …, 2018

… purchased from Avanti lipids. DAPI was purchased from Thermo Fisher
Scientific. Cell Counting Kit-8 (CCK-8) assay was purchased from Dojindo
Molecular Technologies, Inc. 2.2 Synthesis and Characterization of dyes …

Differences in apoptotic signaling and toxicity between dimethylmonothioarsinic acid (DMMTAV) and its active metabolite, dimethylarsinous acid (DMAIII), in HepaRG …

Y Shimoda, K Kato, S Asami, M Kurita, H Kurosawa… - Journal of Trace Elements in …, 2018

… The cytotoxic effects were assayed by WST-8. The cultures in 96-well plates
were placed in 100 µL of medium that contained 10 µL WST-8 reagent
(Cell Counting Kit-8, Dojindo, Kumamoto, Japan) and incubated for 2 hr …

[HTML] Metformin Inhibits TGF-p1-Induced Epithelial-to-Mesenchymal Transition via PKM2 Relative-mTOR/p70s6k Signaling Pathway in Cervical Carcinoma Cells

K Cheng, M Hao - Chemical and Molecular Approach to Tumor …, 2018

… The Cell Counting Kit-8 (CCK-8, Dojindo, Tokyo, Japan) assays were performed to
determine the proliferation of cells … 4.2. Cell Viability Assay Cell viability was detected
by cell counting kit-8 (CCK-8, Dojindo, Tokyo, Japan) assay …

Novel formononetin-7-sal ester ameliorates pulmonary fibrosis via MEF2c signaling pathway

X Zhao, G Qu, C Song, R Li, W Liu, C Lv, X Song… - Toxicology and Applied …, 2018

… danshensu), and 10 µg/mL FS for 48 h. 2.2. Cell counting kit-8 assay (CCK-8). Cell
proliferation was determined using a CCK-8 kit (DOJINDO, Japan) in accordance with
the manufacturer's instructions. In brief, 1 × 10 6 cells/well were …

[HTML] Melatonin protects mouse granulosa cells against oxidative damage by inhibiting FOXO1-mediated autophagy: Implication of an antioxidation-independent …

M Shen, Y Cao, Y Jiang, Y Wei, H Liu - Redox Biology, 2018

… 2.6. Cell viability assay. Cell viability of GCs was measured using Cell
Counting Kit-8 (CCK-8; Dojindo Laboratories, CK04), in which the
tetrazolium salt (WST-8) is reduced by dehydrogenase activities in viable …

Involvement of insulin signalling pathway in methamphetamine-induced hyperphosphorylation of Tau

H Xu, X Chen, J Wang, T Yang, N Liu, J Cheng, R Gao… - Toxicology, 2018

… 2.3. Cell counting kit-8 assay. Cell counting kit-8 was purchased from Dojindo
(Kumamoto, Japan). N2a cells were seeded in 96-well culture plates at a density of
1 × 10 4 cells per well and treated with varied concentrations of METH …

Fluorochloridone perturbs blood-testis barrier/Sertoli cell barrier function through Arp3-mediated F-actin disruption

L Liu, Y Zhang, X Chang, R Li, C Wu, L Tang, Z Zhou - Toxicology Letters, 2018

… 2.7. Cell viability assays. The cytotoxicity of FLC was measured by cell counting
kit-8 assay kit (CCK-8, Dojindo Molecular Technologies, Kumamoto, Japan) as
previously described(Liu et al., 2018). In brief, Sertoli cells, 3 × 10 …

Wet electrospun alginate/gelatin hydrogel nanofibers for 3D cell culture

SS Majidi, PS Adamsen, M Hanif, Z Zhang, Z Wang… - International Journal of …, 2018

… The cell counting kit-8 (CCK-8, Dojindo Laboratories, Kumamoto, Japan) was used to
analyze hEGFP-MSC proliferation. The CCK-8 medium was prepared by diluting the
CCK-8 solution 1:10 with DMEM-high glucose (growth medium) …

[PDF] Plastrum Testudinis Extracts Promote BMSC Proliferation and Osteogenic Differentiation by Regulating Let-7f-5p and the TNFR2/PI3K/AKT Signaling Pathway

GY Shen, H Ren, JJ Huang, ZD Zhang, WH Zhao, X Yu… - Cellular Physiology and …, 2018

… Methods: BMSC proliferation was investigated using the Cell Counting Kit-8 assay … The
proliferation of BMSCs with various concentrations of PTE was measured using the
CCK-8 assay (Dojindo Molecular Technologies, Kumamoto, Japan) …

Effect of humic acid on photofermentative hydrogen production of volatile fatty acids derived from wastewater fermentation

N Xiao, Y Chen, W Zhou - Renewable Energy, 2018

… [25], and activity was expressed as the amount of reduced nitrous oxide (nmol N 2
O/ mL • h). Relative cell viability of PSB was measured with a Cell Counting Kit-8
(CCk-8, Dojindo) according to the manufacturer's instructions [26] …

Overexpression of long noncoding RNA LINC01296 indicates an unfavorable prognosis and promotes tumorigenesis in breast cancer

M Jiang, Y Xiao, D Liu, N Luo, Q Gao, Y Guan - Gene, 2018

… 2.4. Cell proliferation assays. Cell counting kit-8 (CCK-8) and clonogenic assays were
induced to detect cell growth altered by LINC01296 … 10 µl of CCK-8 (Dojindo, Japan)
was added into the corresponding wells at each monitored time …

[PDF] Nobiletin protects PC12 cells from ERS-induced apoptosis in OGD/R injury via activation of the PI3K/AKT pathway

ZR Li, L Yang, J Zhen, Y Zhao, ZN Lu - Experimental and Therapeutic Medicine, 2018

… (Waltham, MA, USA). A Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular
Technologies, Inc. (Kumamoto, Japan). A lactate dehydrogenase (LDH) commer- cial
assay kit was purchased from Shanghai Enzyme-Linked Biotechnology (Shanghai, China) …

Long isoform of VEGF stimulates cell migration of breast cancer by filopodia formation via NRP1/ARHGAP17/Cdc42 regulatory network

M Kiso, S Tanaka, S Saji, M Toi, F Sato - International journal of cancer, 2018

… We performed cell proliferation assay in two ways; direct counting and
WST-8 assay (Cell Counting Kit-8, Dojindo Molecular Technologies,
Inc., Kumamoto). Perimeter measurement Photo images for each cell …

[PDF] Long non-coding RNA FTH 1P3 activates paclitaxel resistance in breast cancer through miR-206/ABCB 1

R Wang, T Zhang, Z Yang, C Jiang, J Seng - Journal of cellular and molecular …, 2018

… After incubation with paclitaxel treatment, the cell viability was mea- sured using cell
Counting Kit-8 (Dojindo, Japan) according to the manufacturer's instructions. 2.4 |
Quantitative real-time polymerase chain reaction (qRT-PCR) …

[HTML] A multifunctional DNA nano-scorpion for highly efficient targeted delivery of mRNA therapeutics

D Li, F Mo, J Wu, Y Huang, H Zhou, S Ding, W Chen - Scientific Reports, 2018

… The cytotoxicity of DNS was evaluated using a cell counting kit-8 (CCK-8) assay with a
series of DNS concentrations and the standard transfection reagent, Lipofectamine 2000
(Lipo2000) … CCK-8 assay was obtained from Dojindo (Shanghai, China) …

Liraglutide Protects Neurite Outgrowth of Cortical Neurons Under Oxidative Stress though Activating the Wnt Pathway

W He, X Tian, M Lv, H Wang - Journal of Stroke and Cerebrovascular Diseases, 2018

… Cell Viability Assay. The viability of cortical neurons was evaluated using Cell Counting
Kit-8 (CCK-8) assay (Dojindo, Kumamoto, Japan). At 24 hours after indicated treatment,
10µl CCK-8 was added to each well, and the plates were incubated for additional 2 hours …

MicroRNA-760 Inhibits Doxorubicin Resistance in Hepatocellular Carcinoma through Regulating Notch1/Hes1-PTEN/Akt Signaling Pathway

T Tian, X Fu, J Lu, Z Ruan, K Nan, Y Yao, Y Yang - Journal of Biochemical and …, 2018

… Cell viability was measured using a cell counting kit-8 (CCK-8) assay … were
transfected with miR-760 mimics or miR-760 inhibitor for 24 h and then
treated with Dox for another 24 h. Afterward, cells were treated with 10 µl …

Attenuation of Na/K-ATPase/Src/ROS amplification signal pathway with pNaktide ameliorates myocardial ischemia-reperfusion injury

H Li, A Yin, Z Cheng, M Feng, H Zhang, J Xu, F Wang… - International Journal of …, 2018

Cell counting kit-8 (CCK-8) assay kit (Dojindo Molecular Technologies, Inc.,
Kumamoto, Japan) was used to determine cell proliferation. H9c2 cells were seeded
at an initial density of 1 × 10 3 cells/well in 96-well plates. Then …

[PDF] Flutamide Induces Hepatic Cell Death and Mitochondrial Dysfunction via Inhibition of Nrf2-Mediated Heme Oxygenase-1

L Zhang, J Guo, Q Zhang, W Zhou, J Li, J Yin, L Cui… - Oxidative Medicine and …, 2018

… Cytotoxicity was evaluated by the determination of cell viability and lactate
dehydrogenase (LDH) leakage. Cell viability was assessed by using a
commercial cell counting Kit-8 (CCK8, Dojindo Molecular Technologies …

Multitargeted kinase inhibitors imatinib, sorafenib and sunitinib perturb energy metabolism and cause cytotoxicity to cultured C2C12 skeletal muscle derived myotubes

VL Damaraju, M Kuzma, CE Cass, CT Putman… - Biochemical Pharmacology, 2018

… DMSO) and diluted for experiments. Carrier solvent was used in controls for
experiments. Cell Counting Kit-8 (CCK-8) was from Dojindo Molecular Technologies,
Inc. (Rockville, MD). CellTiter-Glo Luminescent Cell Viability …

[HTML] MicroRNA-500a Promotes the Progression of Hepatocellular Carcinoma by Post-Transcriptionally Targeting BID

L Bao, M Zhang, S Han, Y Zhan, W Guo, F Teng, F Liu… - Cellular Physiology and …, 2018

… Cell proliferation assay. Cell survival experiments were performed using the Cell
Counting Kit-8 (CCK-8; Dojindo, Shanghai, China) following the manufacturer's
instructions. 2×103 SMMC-7721 or HepG2 cells were seeded …

[PDF] Anti-inflammatory effects of flavonoids in Citrus jabara fruit peels

S Azuma, Y Murakami, E Azuma, K Baba, M Taniguchi - Trends in Immunotherapy, 2018

… Griess reagent system was obtained from Promega (Fitchburg, WI, USA). Cell Counting
Kit-8 was from Dojindo Laboratories (Kumamoto, Japan). RIPA buffer and protease
inhibitor cocktail were purchased from Wako Pure Chemical …

Downregulation of RASSF6 promotes breast cancer growth and chemoresistance through regulation of Hippo signaling

Z He, TT Zhao, F Jin, JG Li, YY Xu, HT Dong, Q Liu… - … and biophysical research …, 2018

… 2 weeks. Then these plates were stained using Giemsa. We perform CCK8 assay
with Cell Counting Kit-8 kit (Dojindo) with a plate reader. 96 well cell plate was
examined at 490 nm. 2.7. Cell cycle analysis. Cell cycle analysis …

[HTML] 3-O-acetyl-11-keto-ß-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase

W Li, J Liu, W Fu, X Zheng, L Ren, S Liu, J Wang, T Ji… - Journal of Experimental & …, 2018

… LDH released from apoptotic cells or dead cells was measured using a
Cytotoxicity LDH Assay Kit (Dojindo, Japan) according to manufacturer's … effect
of AKBA on cell proliferation was assessed by counting viable cells using …

TMEM16A Participates in Hydrogen Peroxide-Induced Apoptosis by Facilitating Mitochondria-Dependent Pathway in Vascular Smooth Muscle Cells

JW Zeng, BY Chen, XF Lv, L Sun, XL Zeng, HQ Zheng… - British journal of …, 2018

… experiments. Page 8. This article is protected by copyright. All rights reserved.
Cell viability assay Cell viability was measured by Cell Counting Assay
Kit-8 (CCK-8, Dojindo Molecular Technologies, MD, Japan) as described …

[PDF] Neuroepithelial transforming gene 1 controls proliferation and migration of non-small cell lung cancer A549 cells via regulating RhoA

S Ding, L Shen, H Huang, Y Tian, Y Zheng, Y Chen… - Int J Clin Exp Med, 2018

… Cell viability Cell viability was confirmed with Cell Counting Kit 8 (CCK-8, Dojindo,
Japan). In brief, A549 cells were planted into 96-well plate (5 × 103/ well). After
transfection, 10 µL CCK-8 solution was added into each well …

[HTML] Anticancer Efficacy of the Combination of Berberine and PEGylated Liposomal Doxorubicin in Meth A Sarcoma-Bearing Mice

J Yahuafai, T Asai, N Oku, P Siripong - Biological and Pharmaceutical Bulletin, 2018

… 0.1–300 µg/mL). After 24, 48 or 72 h of incubation, the components of a Cell Counting
Kit-8 (Dojindo, Kumamoto, Japan) were added to each well according to the
manufacturer's instructions. Absor- bance was measured with …

Cosmetic Method and Dermatological Topical Agent for Use Therein, Migration Imparting Agent, and Method for Screening Components for Use in Cosmetic Method …

T Tsuda, M Yumoto - US Patent App. 15/577,552, 2018

… well plate and cultured at 37° C. for 72 hours under 5% by volume of CO
2 , and then, respiratory activity of the cells was measured as an index for
whether or not the cells were activated, by the WST-8 method using Cell

[PDF] FOXK1 promotes cell growth through activating wnt/ß-catenin pathway and emerges as a novel target of miR-137 in glioma

ZG Ji, HT Jiang, PS Zhang - Am J Transl Res, 2018

… Cell proliferation assay Cell proliferation was determined by using a Cell Counting
Kit-8 (CCK-8) (Dojindo, Kumamoto, Japan) following the manufacturer's guideline. In
brief, cells were seeded in 96-well plates and cultured for 24, 48, 72, and 96 h, respectively …

[PDF] NADH protect against radiation enteritis by enhancing autophagy and inhibiting inflammation through PI3K/AKT pathway

JT Wang, WQ Xie, FQ Liu, Y Bi, XJ Zhu, QE Wang… - Am J Transl Res, 2018

… Cell viability was evaluated by the Cell counting Kit-8 assay (CCK8)
(DOJINDO, JAPAN). IEC-6 normal intestinal epithelial cells (1 × 104 cells/
well) were seeded in the 96-well plate. When the cells stick to the wall …

A novel prognostic biomarker for pancreatic ductal adenocarcinoma: hsa_circ_0001649

Y Jiang, T Wang, L Yan, L Qu - Gene, 2018

… 2.4. Cell counting kit-8 (CCK-8) assays. CCK-8 assays were used to measure cell viability
according to the manufacturer's protocols (Dojindo, Japan). Transfected PANC1 and
BxPC3 cells were seeded in 96-well plates at 2000 cells/well …

[PDF] Long non-coding RNA SNHG1 promotes cell proliferation and invasion by competitively binding to miR-145 in breast cancer

XB Wu, R Wang, WS Wei - Int J Clin Exp Med, 2018

… CCK-8 assay Cell proliferation was documented every 24 h for 4 days using the
Cell Counting Kit-8 (CCK-8; Dojindo, Rockville, MD, USA). Cells were seed- ed in
96-well plates at 2 × 103 cells per well. 10 µl of CCK-8 solution …

[PDF] Long non-coding RNA LncHIFCAR promotes osteoarthritis development via positively regulating HIF-1a and activating the PI3K/AKT/mTOR pathway

J Sun, X Song, L Su, S Cao - Int J Clin Exp Pathol, 2018

… CCK-8 assay Cell proliferation was evaluated using a Cell Counting Kit-8 (CCK-8,
Dojindo Molecular Te- chnologies, Gaithersburg, MD). Briefly, cells were seeded
in 96-well plate with a density of 5000 cells/well. After different …

[PDF] Wnt/ß-catenin deficiency inhibits multiple myeloma cell growth via AMPK/mTOR signaling

N Su, P Wang, Y Liang, Y Li - Int J Clin Exp Med, 2018

… (Beijing, China); Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Molecular
Technologies, Inc. (Shang- hai, China); and antibody against AMPKa was bought from
Epitomics (Abcam) (Cambridge, USA). Cell lines and in vitro culture …

NOVEL TETRAHYDROPYRIDOPYRIMIDINE COMPOUND OR SALT THEREOF

K Minamiguchi, S Okajima, S Aoki, M Asai, T Asai… - US Patent App. 15/893,804, 2018

A method for inhibiting androgen activity, including administering an effective
amount of a tetrahydropyridopyrimidine compound of formula (I) or a
pharmaceutically acceptable salt thereof to a subjec.

Classic SRY-box protein SOX7 functions as a tumor suppressor regulating WNT signaling and is methylated in renal cell carcinoma

L Wang, Y Fan, L Zhang, L Li, G Kuang, C Luo, C Li… - The FASEB Journal, 2018

Cell Counting Kit-8 assay Cell growth was measured using a Cell Counting
Kit-8 (Dojindo, Kumamoto, Japan) in 96-well plates (1000 cells per well),
according to the manufacturer's instructions. 2 Vol. 33 January 2019 WANG …

Long noncoding RNA HOXD-AS1 promotes non-small cell lung cancer migration and invasion through regulating miR-133b/MMP9 axis

H Xia, H Jing, Y Li, X Lv - Biomedicine & Pharmacotherapy, 2018

… Cell proliferation was measured using the cell counting kit-8 (CCK-8;Dojindo,
Japan) according to the manufacturer's protocol. Optical density (OD) at the
wavelength of 450 nm was measured using a Benchmark PlusTM microplate …

[HTML] lncRNA H19 predicts poor prognosis in patients with melanoma and regulates cell growth, invasion, migration and epithelial–mesenchymal transition in melanoma …

G Shi, H Li, F Gao, Q Tan - OncoTargets and therapy, 2018

… Furthermore, in vitro functional roles of H19 in melanoma cell lines were
examined by Cell Counting Kit 8 (CCK-8) assay, cell invasion … Cell growth
was assessed by using CCK-8 assay (Dojindo Molecular Technologies …

[PDF] Long non-coding RNA Linc00312 modulates the sensitivity of ovarian cancer to cisplatin via the Bcl-2/Caspase-3 signaling pathway

C Zhang, M Wang, C Shi, F Shi, C Pei - Bioscience trends, 2018

… 2.5. Cell counting kit-8 assay (CCK-8 assay) … Cells were incubated for 48 h. Cell viability
was assessed using the CCK-8 assay (Dojindo Laboratories, Shanghai, China).
After 1 h, the P2 Table 1. The primers for RT-qPCR Name …

Heparin impairs angiogenic signaling and compensatory lung growth after left pneumonectomy

DT Dao, L Anez-Bustillos, J Ourieff, A Pan, PD Mitchell… - Angiogenesis, 2018

… After 3 days of incubation at 37 °C, cell viability was assessed with Cell Counting Kit-8
(Dojindo Molecular Technologies, Rockville, MD) and colorimetric signal was detected
with FLUOstar Omega microplate reader. Assessment of anticoagulation functions …

3D printing human induced pluripotent stem cells with novel hydroxypropyl chitin bioink: scalable expansion and uniform aggregation

Y Li, X Jiang, L Li, ZN Chen, G Gao, R Yao, W Sun - Biofabrication, 2018

… 2.9 Cell proliferation analysis 10% (v/v) Cell Counting Kit-8 (CCK-8, Dojindo, CK04) was
used to measure hiPSC proliferation in the samples from all fourteen groups on Day 1,
Day 4, Day 7 and Day 10, according to the manufacturer's instructions …

Alpha-mangostin inhibits the migration and invasion of A549 lung cancer cells

TKT Phan, F Shahbazzadeh, TTH Pham, T Kihara - PeerJ, 2018

… (Tokyo, Japan). a-Mangostin was purchased from Wako Pure Chemical Industries Ltd.
(Osaka, Japan). Cell counting kit-8 was purchased from Dojindo Molecular Technologies,
Phan et al. (2018), PeerJ, DOI 10.7717/peerj.5027 2/17 Page 3. Inc. (Kumamoto, Japan) …

Curcumin attenuates resistance to irinotecan via induction of apoptosis of cancer stem cells in chemoresistant colon cancer cells

P Su, Y Yang, G Wang, X Chen, Y Ju - International Journal of Oncology, 2018

… A Cell Counting kit-8 (CCK-8) assay (Dojindo Molecular Technologies,
Inc., Kumamoto, Japan) was used to assess the inhibition of cell growth
in response to CPT-11 (0, 5, 10, 20, 40, 80, 120, 160 and 200 µM) and …

Enhanced expression of lncRNA TP73-AS1 predicts adverse phenotypes for cholangiocarcinoma and exerts oncogenic properties in vitro and in vivo

Y Yao, Y Sun, Y Jiang, L Qu, Y Xu - Biomedicine & Pharmacotherapy, 2018

Cell counting Kit-8 (CCK-8) assays. CCK-8 assays were used to measure
CCA cell viability altered by TP73-AS1. Transfected QBC939 and Huh-28
cells were seeded in 96-well plates at 1500 cells/well. 10 µl of CCK-8 solution …

[PDF] Long noncoding RNA SNHG1 promotes cell proliferation through PI3K/AKT signaling pathway in pancreatic ductal adenocarcinoma

Y Zhang, R Zhang, G Luo, K Ai - Journal of Cancer, 2018

… Cell proliferation assays Cell proliferation assays were performed with Cell Counting
Kit-8 (Dojindo, Japan) according to the manufacturer's protocol. The proliferative ability
of cells was detected every 24h. Absorbance at 450nm …

MiR-126 correlates with increased disease severity and promotes keratinocytes proliferation and inflammation while suppresses cells' apoptosis in psoriasis

S Feng, L Wang, W Liu, Y Zhong, S Xu - Journal of Clinical Laboratory Analysis, 2018

Cell Counting Kit- 8 and annexin V/propidium iodide assays were performed
to assess the cells' proliferation and apoptosis, and protein levels of apoptotic
markers (cleaved caspase- 3 [C- caspase- 3] and B … Cells' proliferation was …

Long non-coding RNA PVT1 promotes cell proliferation and invasion through regulating miR-133a in ovarian cancer

Q Yang, Y Yu, Z Sun, Y Pan - Biomedicine & Pharmacotherapy, 2018

… 1,si-PVT1#2, or si-Ctrl. Cell proliferation was determined using Cell Counting Kit-8
(CCK-8) kit (Dojindo Laboratories, Kumamoto, Japan) according to the manufacturer's
instructions. The absorbance of each well at a wavelength …

The E3 ubiquitin ligase RNF146 promotes colorectal cancer by activating the Wnt/ß-catenin pathway via ubiquitination of Axin1

J Shen, Z Yu, N Li - Biochemical and biophysical research communications, 2018

… Cell proliferation was evaluated using Cell Counting Kit-8 (CCK-8; Dojindo, Japan)
according to the manufacturer's instructions. Cell apoptosis was evaluated by flow
cytometry using an Annexin V/7-AAD Apoptosis Detection Kit (Keygentec, Nanjing, China) …

[HTML] Fish oil-derived lipid emulsion induces RIP1-dependent and caspase 8-licensed necroptosis in IEC-6 cells through overproduction of reactive oxygen species

JK Yan, WH Yan, W Cai - Lipids in Health and Disease, 2018

… In some experiments, cells were pre-treated with the following chemicals: Z-IETD-FMK
(20 µM), Nec-1 (20 µM) and NSA (10 µM). Cell viability assay. Cell viability was evaluated
using the Cell Counting Kit-8 (CCK-8) assay kit (Dojindo, Japan) …

[PDF] In Vivo Study of Natural Killer (NK) Cell Cytotoxicity Against Cholangiocarcinoma in a Nude Mouse Model

INHYE JUNG, KIM DO HEE, YOO DA KYUNG… - In Vivo, 2018

… Cytotoxicity assay. Cytolytic NK cell activity was measured by using Cell
Counting Kit-8 (CCK-8) (Dojindo Mol. Tech., Rockville, MD, USA). K562 cells
were included as a positive target cell to compare cytolytic activity of the NK …

[HTML] Co-culturing nucleus pulposus mesenchymal stem cells with notochordal cell-rich nucleus pulposus explants attenuates tumor necrosis factor-a-induced senescence

XC Li, MS Wang, W Liu, CF Zhong, GB Deng, SJ Luo… - Stem Cell Research & …, 2018

… To measure cell proliferation, a Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Japan)
was used as previously described [3]. Briefly, NPMSCs were seeded in 24-well plates
(1 × 10 4 cells/well) and different groups were incubated for 1, 3, 5, and 7 days …

[HTML] ClC-2 knockdown prevents cerebrovascular remodeling via inhibition of the Wnt/ß-catenin signaling pathway

J Lu, F Xu, Y Zhang, H Lu, J Zhang - Cellular & Molecular Biology Letters, 2018

… Cell proliferation assay. The viability of HBVSMCs was measured with a Cell Counting
Kit-8 (CCK-8; Dojindo Laboratories) according to the manufacturer's instructions.
Briefly, HBVSMCs cells were seeded in 96-well plates (2 …

[HTML] Screening of a library of traditional Chinese medicines to identify anti-malarial compounds and extracts

M Nonaka, Y Murata, R Takano, Y Han, MHB Kabir… - Malaria Journal, 2018

… IC 50 was determined by analysis of dose–response curve made by GraphPad Prism
(GraphPad Software, CA, USA). Cytotoxicity assays. Cell Counting Kit-8 (Dojindo,
Kumamoto, Japan) was used for the cytotoxicity assays …

[PDF] Establishment and Characterization of a Highly Metastatic Ovarian Cancer Cell Line

J Ruibin, C Guoping, Z Zhiguo, N Maowei, W Danying… - BioMed Research …, 2018

… The cell viability was determined 48 hours later with cell-Counting kit-8 (CCK8, Dojindo,
Japan) according to the manufacturer's instruction. 2.1.8. Flow Cytometry Analysis. Log
phase growing A2780 and A2780-M cells were collected and washed with PBS …

Long-Term Effects of Tourmaline on Nitrogen Removal from Wastewater and Bacterial Community Shift in Activated Sludge at Low Temperatures

H Yahong, Q Shan, M Fang, W Jue, Q Yilun, A Xuedi - Water, Air, & Soil Pollution, 2018

… 1 EDX analysis of UTPs (a) and FE-SEM image of UTPs (b). 2.2 Addition Dosage of
Tourmaline. The cytotoxicity of UTPs towards activated sludge was investigated using
the cell counting kit-8 (CCK-8, Dojindo, JPN) according to manufacturer's instructions …