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The multi-functional organelle is not only involved in energy production in a cell, but other additional cellular functions. The active cycle of mitochondrial fusion and division induces morphological changes, which is called mitochondrial dynamics. Abnormalities in morphological control of mitochondria are associated with neurodegenerative diseases, metabolic disorders, aging, and so on. Therefore, the demand for long-term observation of mitochondrial dynamics has recently been increasing.





Mitophagy
Reagent Mtphagy Dye Keima-Red
Principle Mtphagy Dye (included in Mitophagy Detection Kit) is a pH-sensitive fluorescent probe that accumulates in mitochondria and emits red fluorescence due to acidic conditions in a lysosome. A pH-sentitive ratiometric fluorescent protein. The excitation spectrum changes accoring to pH. This protein shows high fluorescence ratio (Ex. 550 nm/440 nm) values in a lysozome.
Fixed cell staining - -
Live-cell staining Yes Yes
Fixation after live-cell staining - -
Staining time > 30 min -
Ex / Em 530 / 700 440, 550 / 620
Product code MD01 , MT02 -
Lipophilic peroxide Singlet oxygen Superoxide
Reagent MitoPeDPP Si-DMA MitoSOX
Principle A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with lipophilic peroxides in mitochondria to emit fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with singlet oxigen generated in mitochondria to emit red fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and reacts with superoxide generated in mitochondria to emit red fluorescence.
Fixed cell staining - - -
Live-cell staining Yes Yes Yes
Fixation after live-cell staining - - -
Staining time > 15 min > 45 min > 10 min
Ex / Em 452 / 470 644 / 670 510 / 590
Product code M466 MT05 -
Membrane potential Iron ion (Fe2+) Calcium ion (Ca2+)
Reagent JC-1 MT-1 TMRM, TMRE Mito-FerroGreen Rhod2-AM
Principle A cell-permeant ratiometric fluorescent dye that accumulates in intact mitochondria due to the membrane potential. The excitation spectrum changes according to the mitochondria membrane potential. Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. MT-1 is extremely photostable and more sensitive than JC-1 and can provide equivalent detection sensitivity to TMRE. Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. Diffusion of the probes occurs in a damaged mitochondria that has the decreased membrane potential. A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with ferrous ion in mitochondria to emit green fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with calcium ion in mitochondria to emit red fluorescence.
Fixed cell staining - - - - -
Live-cell staining Yes Yes Yes Yes Yes
Fixation after live-cell staining - Yes - - -
Staining time 30-60 min 30 min 30-60 min 30 min 30-60 min
Ex / Em Monomer: 514 / 529
J-aggregation: 585/590 		530-560 / 570-640 550 / 575 505 / 535 553 / 576
Product code MT09 MT13 - M489 R002
Mitochondria staining
Reagent MitoBright LT series MitoBright IM Red MitoTracker series MitoRed Rhodamine 123
Principle Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential and covalently binds to proteins and other biomolecules. Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential. Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential.
Fixed cell staining - - - - -
Live-cell staining Yes Yes Yes Yes Yes
Fixation after live-cell staining Yes Yes - - -
Staining time 30 min 30 min 15-45 min > 15 min > 15 min
Ex / Em 493 / 508, 547 / 563, 643 / 663 548 / 566 490 / 516 ~ 644 / 665 560 / 580 507 / 529
Product code MT10 , MT11 , MT12 MT15 - R237 R233


Application Products
Mitophagy Detection Mitophagy Detection Kit
Mitochondrial Phospholipid Peroxidase Detection MitoPeDPP
Mitochondrial Ferrous Ion Detection Mito-FerroGreen
Singlet oxygen specific fluorescent probe Si-DMA for Mitochondrial Singlet Oxygen Imaging
Mitochondrial Membrane Potential JC-1 MitoMP Detection Kit
MT-1 MitoMP Detection Kit
Mitochondria Staining MitoBright LT Red
MitoBright LT Deep Red
MitoBright LT Green
MitoBright IM Red for Immunostaining
-Cellstain- MitoRed
-Cellstain- Rh123
Mitochondrial Superoxide Detection mtSOX Deep Red – Mitochondrial Superoxide Detection