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-Cellstain-AO Solution -Cellstain-AO Solution, 3,6-Bis(dimethylamino)acridine, hydrochloride, aqueous solution
Item #: A430 -

Product Description Acridine orange (AO) forms a complex with double-stranded DNA to emit green fluorescence (Fig. 1). AO also forms a complex with singlestranded DNA or RNA to emit red fluorescence. One molecule of AO intercalates with three base pairs of double-stranded DNA and emits green fluorescence with the maximum wavelength at 526 nm (excitation 502 nm). One molecule of AO can also interact with one phosphate group of single-stranded DNA or RNA to form an aggregated, or stacked,...

-Cellstain-Calcein-AM -Cellstain-Calcein-AM, 3,6-Di(O-acetyl)-4,5-bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein tetraacetoxymethyl ester [CAS: 148504-34-1]
Item #: C326 -

Product Description Calcein-AM readily passes through the cell membrane of viable cells because of its enhanced hydrophobicity compared to Calcein. After Calcein-AM permeates into the cytoplasm, it is hydrolyzed by esterases to Calcein, which remains inside the cell (Fig. 1). Among other reagents, including BCECF-AM and Carboxy-fluorescein diacetate, Calcein-AM is the most suitable fluorescent probe for staining viable cells because of its low cytotoxicity. Calcein does not inhibit any...

-Cellstain-Calcein-AM Solution -Cellstain-Calcein-AM solution, 3,6-Di(O-acetyl)-4,5-bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein tetraacetoxymethyl ester, DMSO solution
Item #: C396 -

Product Description Calcein-AM readily passes through the cell membrane of viable cells because of its enhanced hydrophobicity compared to Calcein. After Calcein-AM permeates into the cytoplasm, it is hydrolyzed by esterases to Calcein, which remains inside the cell (Fig. 1). Among other reagents, including BCECF-AM and Carboxy-fluorescein diacetate, Calcein-AM is the most suitable fluorescent probe for staining viable cells because of its low cytotoxicity. Calcein does not inhibit any...

-Cellstain-CFSE -Cellstain-CFSE, 5- or 6-(N-Succinimidyloxycarbonyl)-fluorescein 3,6 diacetate [CAS: 150347-59-4]
Item #: C375 -

Product Description CFSE is cell-membrane permeable and readily accumulates inside viable cells where it covalently attaches to intracellular proteins (Fig. 1). Hydrolyzed CFSE emits fluorescence and covalently attached fluorescein molecules do not leak from cells. CFSE-labeled cells can be monitored over several weeks in vivo. Therefore, CFSE is utilized for detection of viable cell as well as for the long-term observation of cell activities by fluorescent microscopy. The excitation and...

-Cellstain-CytoRed Solution -Cellstain-CytoRed Solution, 7-Isobutyloxycarbonyloxy-3H-phenoxazin-3-one
Item #: C410 -

Product Description CytoRed is cell membrane permeable and accumulates inside of viable cells as resorufin (Fig. 1). CytoRed has a much wider spectrum than BCECF or Calcein, so filters for fluorescein and rhodamine can also be used. The excitation and emission wavelengths of resorufin are 560 nm and 590 nm, respectively. A photo of cells stained with CytoRed is shown in Fig. 2. Fig. 1 Cell staining mechanism ...

-Cellstain-DAPI -Cellstain-DAPI, 4,6-Diamidino-2-phenylindole, dihydrochloride [CAS: 28718-90-3]
Item #: D212 -

Product Description DAPI is an AT-sequence-specific DNA intercalator that attaches to DNA at the minor groove of the double helix like Hoechst dyes. DAPI is not permeable through viable cell membranes, but it passes through disturbed cell membranes to stain the nucleus. DAPI has a high photobleaching tolerance level. DAPI is utilized for the detection of mitochondrial DNA in yeast, chloroplast DNA, virus DNA, micoplasm DNA, and chromosomal DNA. The excitation and emission wavelengths of...

-Cellstain-DAPI Solution -Cellstain-DAPI Solution, 4,6-Diamidino-2-phenylindole, dihydrochloride, aqueous solution
Item #: D523 -

Product Description DAPI is an AT-sequence specific DNA intercalator that attaches to DNA at the minor groove of the double helix like Hoechst dyes. Though DAPI is not permeable through viable cell membranes, it passes through disturbed cell membranes to stain the nucleus. DAPI has a high photo-bleaching tolerance level. DAPI is utilized for the detection of mitochondrial DNA in yeast, chloroplast DNA, virus DNA, micoplasm DNA and chromosomal DNA. The excitation and emission wavelengths of...

-Cellstain-Double Staining Kit -Cellstain-Double Staining Kit
Item #: CS01 -

Product Description -Cellstain-Double Staining Kit is utilized for simultaneous fluorescence staining of viable and dead cells. This kit contains Calcein-AM and Propidium Iodide (PI) solutions, which stain viable and dead cells, respectively (Fig. 1). Calcein-AM, an acetoxymethyl ester of calcein, is highly lipophilic and cell membrane permeable. Although Calcein-AM itself is not a fluorescent molecule, the calcein generated from Calcein- AM by esterase in a viable cell emits a strong green...

-Cellstain-FDA -Cellstain-FDA, Fluorescein diacetate [CAS: 596-09-8]
Item #: F209 -

Product Description FDA is cell-membrane permeable and accumulates inside of viable cells as fluorescein (Fig. 1). Since fluorescein is less hydrophilic than BCECF or Calcein, the leakage of fluorescein from cells is rather high. FDA is also utilized for flow cytometry. The excitation and emission wavelengths of fluorescein are 488 nm and 530 nm, respectively. FDA-stained cells are shown in Fig. 2. Fig. 1 Cell staining mechanism ...

-Cellstain-Hoechst 33258 Solution -Cellstain-Hoechst 33258 Solution, Bisbenzimide, 2’-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole trihydrochloride, aqueous solution
Item #: H341 -

Product Description Hoechst dyes are cell membrane permeable and stain DNA to emit intense blue fluorescence. They bind to DNA in the minor groove of poly-AT sequence rich areas. Both Hoechst 33342 and Hoechst 33258 are water-soluble and stable in aqueous solutions. The excitation and emission wavelengths of Hoechst-DNA complex are 350 nm and 460 nm, respectively. Chemical Structure ...

-Cellstain-Hoechst 33342 Solution -Cellstain-Hoechst 33342 Solution, Bisbenzimide, 2’-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole trihydrochloride, aqueous solution
Item #: H342 -

Product Description Hoechst dyes are cell membrane permeable and stain DNA to emit intense blue fluorescence. They bind to DNA in the minor groove of poly-AT sequence rich areas. Both Hoechst 33342 and Hoechst 33258 are water-soluble and stable in aqueous solutions. The excitation and emission wavelengths of Hoechst-DNA complex are 350 nm and 460 nm, respectively. Chemical Structure ...

-Cellstain-PI -Cellstain-PI, 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide [CAS: 25535-16-4]
Item #: P346 -

Product Description Propidium iodide (PI) is an ethidium bromide analog that emits red fluorescence upon intercalation with double-stranded DNA. PI does not permeate viable cell membranes, but passes through disturbed cell membranes and stains the nuclei. PI is often used in combination with a fluorescein compound, such as Calcein-AM or FDA, for simultaneous staining of viable and dead cells. The excitation and emission wavelengths of PI-DNA complex are 535 nm and 615 nm, respectively....

-Cellstain-PI Solution -Cellstain-PI Solution, 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide, aqueous solution
Item #: P378 -

Product Description Propidium iodide (PI) is an ethidium bromide analog that emits red fluorescence upon intercalation with double-stranded DNA. PI does not permeate viable cell membranes, but passes through disturbed cell membranes and stains the nuclei. PI is often used in combination with a fluorescein compound, such as Calcein-AM or FDA, for simultaneous staining of viable and dead cells. The excitation and emission wavelengths of PI-DNA complex are 535 nm and 615 nm, respectively....

BCECF-AM BCECF-AM, 3-O-Acetyl-2,7-bis(carboxyethyl)-4 or 5-carboxyfluorescein, diacetoxymethyl ester [CAS:117464-70-7]
Item #: B262 -

Product Description BCECF is the most widely used intracellular pH probe. Dr. Tsien and others improved this carboxyfluorescein by introducing two extra carboxylates that allow it to be retained better by the cell. BCECF is highly water-soluble because it has 4 to 5 negative charges at neutral pH; it becomes difficult to pass through the cell membrane after loading. Its pKa value, 6.97, is higher than that of carboxyfluorescein. BCECF has an isosbestic point at 439 nm in the excitation...

CFSE -Cellstain-CFSE, 5- or 6-(N-Succinimidyloxycarbonyl)-fluorescein 3,6 diacetate [CAS: 150347-59-4]
Item #: C309 -

Product Description CFSE is cell-membrane permeable and readily accumulates inside viable cells where it covalently attaches to intracellular proteins (Fig. 1). Hydrolyzed CFSE emits fluorescence and covalently attached fluorescein molecules do not leak from cells. CFSE-labeled cells can be monitored over several weeks in vivo. Therefore, CFSE is utilized for detection of viable cell as well as for the long-term observation of cell activities by fluorescent microscopy. The excitation and...

ECGreen-Endocytosis Detection ECGreen-Endocytosis Detection, E296
Item #: E296 -

Product Description Endocytosis is a cellular process that involves macromolecules being taken up through a plasma membrane-derived vesicle called an endosome. The endocytic pathway contributes to the maintenance of intracellular homeostasis by bringing in various nutrients to the cell and transporting unwanted components to the lysosome, which acts as a waste disposal system. Recent findings reveal that disruption of endocytosis is related to certain neurodegenerative disorders and immune...

ExoSparkler Exosome Membrane Labeling Kit Exosome, Exosome Membrane, ExoSparkler, Green, EX01
Item #: EX01 -

EX01 EX02 EX03 Product DescriptionExosomes are a form of extracellular vesicle (EV), which could contribute to malignant transformation and the metastasis of cancer. Consequently, intercellular communication via exosomes is attracting considerable interest in the scientific community. To shed light on such communication, labeling techniques based on fluorescent dyes have been used. Fluorescent dyes that label the cellular membrane are commonly used for exosome labeling because the lipid...

ExoSparkler Exosome Protein Labeling Kit Exosome, Exosome Protein, ExoSparkler, Green, EX04
Item #: EX04 -

EX04 EX05 EX06 Product DescriptionExosomes are a form of extracellular vesicle (EV), which may contribute to malignant transformation and the metastasis of cancer. Consequently, intercellular communication via exosomes is attracting considerable interest in the scientific community. To shed light on such communication, labeling techniques based on fluorescent dyes have been used. Fluorescent dyes that label the cellular membrane are commonly used for exosome labeling because the lipid bilayer...

PlasMem Bright Green PlasMem Bright Green, P504
Item #: P504 -

Detection Principle The plasma membrane (PM), consists of a lipid bilayer separating the intracellular environment from the extracellular space. Consequently, the PM plays a central role in many cell behaviors, such as cell migration, cell stretching, and signaling cascades. Additionally, PM dysfunction is an important biomarker because it is related to the cell status and is linked to many diseases. Dojindo’s PlasMem Bright dyes overcome these limitations. PlasMem Bright dyes are designed to...

PlasMem Bright Red PlasMem Bright Red, P505
Item #: P505 -

Detection Principle The plasma membrane (PM), consists of a lipid bilayer separating the intracellular environment from the extracellular space. Consequently, the PM plays a central role in many cell behaviors, such as cell migration, cell stretching, and signaling cascades. Additionally, PM dysfunction is an important biomarker because it is related to the cell status and is linked to many diseases. Dojindo’s PlasMem Bright dyes overcome these limitations. PlasMem Bright dyes are designed to...