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Fura 2-AM Fura 2-AM, 1-[6-Amino-2-(5-carboxy-2-oxazolyl)-5-benzofuranyloxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N’,N’- tetraacetic acid, pentaacetoxymethyl ester [CAS: 108964-32-5]
Item #: F015 -

Product Description Fura 2 was developed to improve the fluorescent properties of Quin 2. The signal intensity in 1 mM of loaded Fura 2 corresponds to that of 30 mM of loaded Quin 2. This allows an experiment at a lower concentration of indicator using Fura 2 instead of Quin 2. Fura 2 is one of the most widely used calcium indicators for ratiometric measurement. Many types of instrumentation are now available for experiments using Fura 2, but Fura 2 is especially suitable for digital imaging...

Glucose Assay Kit-WST  G264, G264-05, G264-20, Glucose
Item #: G264 -

Principle This kit can detect glucose found within cell culture medium or intracellular glucose by measuring the absorbance of the colored WST formazan dye. The degree of absorbance depends on the amount of glucose present within the sample.This kit contains a glucose standard solution that can be used to create a standard curve. This allows for the quantitation of glucose levels present in the sample. --> Procedure Procedures are so easy that you simply incubate the plate after the addition...

Glucose Uptake Assay Kit-Green Glucose Uptake Assay Kit-Green
Item #: UP02 -

Nutrient metabolism is necessary for energy production in cells and regulates various cellular functions, including gene expression. Glucose is one of the key substrates for the generation of ATP and to sustain cellular homeostasis. Thus, glucose metabolism has been the subject of intense investigations. In cancer research, tumor cells enhance glucose uptake and consumption for their growth and proliferation. Therefore, elevated glucose uptake is a marker of tumors, and glucose transporters...

Glutamate Assay-Kit-WST   Glutamate,  Glutamate Assay-Kit-WST , measurement, detection, glutamate ratio, reduced, oxidized, antioxidant
Item #: G269 -

--> Product Description Glutamate is an important neurotransmitter that contributes to the biosynthesis of protein and glutathione. An excess of glutamate is considered to be a cause of neurodegenerative disorders, including Alzheimer's disease. A recent study showed that an iron-dependent cell death called “ferroptosis” is induced by inhibition of cystine/glutamate antiporter (xCT) responsible for cystine uptake and glutamate release. As such, cancer research which targets xCT has also been...

Glutamine Assay-Kit-WST  Glutamine,  Glutamine Assay-Kit-WST , measurement, detection, glutamine ratio, reduced, oxidized, antioxidant
Item #: G268 -

--> Product Description Glutamine, a source of α-ketoglutarate (one of the tricarboxylic acid (TCA) cycle intermediates), is an important substance used for energy production and the syntheses of nucleic acids and other amino acids. Glutaminolysis, the process by which glutamine as a substrate is converted to α-ketoglutarate, is upregulated in cancer cells. A study reports that glutaminolysis has largely contributed to scavenging of reactive oxygen species and reduction of oxidized...

HilyMax Transfection, Transfection Reagent, DNA transfection, Stem cell transfection, Drosophila transfection, Insect cell transfection
Item #: H357 -

Description:Various methods have been developed to express specific proteins in mammalian cells. The first method to introduce DNA to cells was calcium phosphateprecipitation. However, the transfection efficiency was very poor and there was a high rate of cell-to-cell variation. The second method introduced was the DEAEsephadex method. The transfection efficiency drastically improved, but still the method could not be used for all cells and required heavy metalions to enhance transfection...

Ins(1,4,5)P3 (synthetic) Ins(1,4,5)P3 (synthetic), D-myo-Inositol-1,4,5-triphosphate, tripotassium salt [CAS: 85166-31-0]
Item #: I007 -

Product Description Inositol phosphates and diacylglycerols are generated from phosphatidyl inositols by phospholipase C. They are involved in cellular calcium ion movements as second messengers. Inositol-1,4,5-triphosphate(Ins(1,4,5)P3) is an extremely important messenger that triggers calcium ion release from endoplasmic and sarcoplasmic reticulum. It is thought to increase the calcium sensitivity of the inositol- induced calcium release(IICR) channel in the intracellular calcium source....

Lactate Assay Kit-WST  L256, L256-10,Lactate
Item #: L256 -

Lactate Assay Kit-WST can quantify lactate (the lower limit: 0.02 mmol/l), one of the glycolysis metabolites. Because the Lactate Assay Kit-WST corresponds to 96 well micro-plates, it allows for multiple samples to be measured at once. Lactate quantification has been widely used as a marker for monitoring changes in intracellular metabolic pathway in many different areas of cancer research. In recent years, there have been an increasing number of reports on using lactate as an indicator of...

Lipi-Series: Lipid Droplet Detection Probes(LD01, LD02, LD03, LD04) LD01, LD01-10, Lipi-Blue, Lipid Droplet
Item #: LD01 -

LD01 LD02 LD03 LD04 Product Description Lipid droplets (LDs) are composed of neutral lipids such as triacylglycerol & cholesteryl ester that are surrounded by phospholipid monolayers and are seen ubiquitously, not only in adipocytes1). LDs were originally thought to serve as a lipid storage unit, until a recent study showing that LDs play an important role in regulating lipid metabolism, autophagy2) and cellular senescence3). Therefore, LDs have gained great attention as an important tool to...

Lipid Droplet Assay Kit-Blue Lipid Droplet, LD05, LD05-10, Lipid Droplet Assay Kit-Blue
Item #: LD05 -

Product Description Lipi probes are small molecule which emit strong fluorescence in hydrophobic environment such as in LDs. Function of lipid droplets   Lipid droplets (LDs) are composed of neutral lipids such as triacylglycerol & cholesteryl ester that are surrounded by phospholipid monolayers and are seen ubiquitously, not only in adipocytes1). Although LDs were simply thought to serve as a lipid storage unit, a recent study has stated that LDs play an important role in regulating...

Lipid Droplet Assay Kit-Deep Red Lipid Droplet, LD06, LD06-10, Lipid Droplet Assay Kit-Deep Red
Item #: LD06 -

Product Description Lipi probes are small molecule which emit strong fluorescence in hydrophobic environment such as in LDs. Function of lipid droplets   Lipid droplets (LDs) are composed of neutral lipids such as triacylglycerol & cholesteryl ester that are surrounded by phospholipid monolayers and are seen ubiquitously, not only in adipocytes1). Although LDs were simply thought to serve as a lipid storage unit, a recent study has stated that LDs play an important role in regulating...

Microbial Viability Assay Kit-WST Microbial Viability Assay Kit-WST, Bacteria, Colony counting, Fungi, Yeast cell viabilty, XTT, CLSI method, Bacteria contamination, Agar plate, Fukuoka Industrial Technology Center,E.coli, B.subtilis, S.aureus, L.casei, S.cerevisiae, MRSA, Oxacillin, Clinical and Laboratory Standards Institute, Tadayuki Tsukatani
Item #: M439 -

Product Description Viable bacterial cell detections are very important for analyzing bacteria contamination in food or evaluating the cleanliness of facilities in order to protect us from food poisoning and infections. Bacterial cell detections are also used for the screening of sanitizing agents and drug resistance detections. Generally, counting the number of colonies on an agar plate is the standard method for determining the number of viable bacterial cells in samples. However, colony...

MQAE MQAE, N-Ethoxycarbonylmethyl-6-methoxyquinolinium bromide [CAS: 124505-60-8]
Item #: M024 -

Product Description MQAE with bromide ion as a counteranion has fluorescence excitation at 355 nm and emission at 460 nm. The fluorescent intensity of MQAE decreases in proportion to the chloride increase in cells. Chloride ion concentrations less than 50 mM may be monitored without being affected by pH changes. MQAE has high water solubility and membrane permeability. Chemical Structure ...

NAD/NADH Assay Kit-WST N509, N509-10,NAD/NADH
Item #: N509 -

Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NAD+ levels can be determined by subtracting NADH levels from independently measured total NAD+/NADH levels. Principle: NAD/NADH Assay Kit-WST enables quantification of the amount of total NAD+/NADH, NADH and NAD+ in cells and measurement of...

NADP/NADPH Assay Kit-WST NADP/NADPH Assay Kit-WST, N510
Item #: N510 -

Principle: NADP/NADPH Assay Kit-WST enables quantitation of the amount of total NADP+/NADPH, NADPH and NADP+ in cells and measurement of their ratio. Sensitive Measurements of NADP+ and NADPH Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADPH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NADP+ levels can be determined by subtracting NADPH...

Nucleolus Bright Green Nucleolus staining, N511, N512
Item #: N511 -

Detection Principle A Nucleolus is one of the structures forming a nucleus and becoming the starting point of the ribosomal biogenesis. There are many ribosomal RNA (rRNA) in the nucleolus and transcription of rRNA and processing are carried out. Whereas the morphological change of nucleolus is known as one of the indicator of cancer diagnosis, there has been some scientific reports describing the relation between nucleolus and DNA damge, autophagy, virus infection, and cellular senescence....

Nucleolus Bright Red Nucleolus staining, N511, N512
Item #: N512 -

Detection Principle A Nucleolus is one of the structures forming a nucleus and becoming the starting point of the ribosomal biogenesis. There are many ribosomal RNA (rRNA) in the nucleolus and transcription of rRNA and processing are carried out. Whereas the morphological change of nucleolus is known as one of the indicator of cancer diagnosis, there has been some scientific reports describing the relation between nucleolus and DNA damge, autophagy, virus infection, and cellular senescence....

PlasMem Bright Green PlasMem Bright Green, P504
Item #: P504 -

Detection Principle The plasma membrane (PM), consists of a lipid bilayer separating the intracellular environment from the extracellular space. Consequently, the PM plays a central role in many cell behaviors, such as cell migration, cell stretching, and signaling cascades. Additionally, PM dysfunction is an important biomarker because it is related to the cell status and is linked to many diseases. Dojindo’s PlasMem Bright dyes overcome these limitations. PlasMem Bright dyes are designed to...

PlasMem Bright Red PlasMem Bright Red, P505
Item #: P505 -

Detection Principle The plasma membrane (PM), consists of a lipid bilayer separating the intracellular environment from the extracellular space. Consequently, the PM plays a central role in many cell behaviors, such as cell migration, cell stretching, and signaling cascades. Additionally, PM dysfunction is an important biomarker because it is related to the cell status and is linked to many diseases. Dojindo’s PlasMem Bright dyes overcome these limitations. PlasMem Bright dyes are designed to...

Quin 2 Quin 2, 8-Amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N’,N’-tetraacetic acid, tetrapotassium salt [CAS: 73630-23-6]
Item #: Q001 -

Product Description Quin 2 forms a stable fluorescent complex with calcium (logKCaY=7.1), but not with magnesium (logMgY=2.7). The complex has a high quantum yield (0.14) at emission wavelength 525 nm, excitation wavelength 339 nm, and emission wavelength 492 nm. Quin 2-AM is an acetoxymethyl ester derivative of Quin 2 that is readily permeable through cell membranes. Within the cell, the ester is hydrolyzed to Quin 2. Calcium Chelation ...

Rhod 2 Rhod 2, 1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N’,N’- tetraacetic acid, chlorid [CAS: 132523-91-2]
Item #: R001 -

Product Description Rhod 2 has the longest fluorescent wavelength signal of all the calcium indicators. It has a rhodamine-like fluorophore whose excitation and emission maxima are 557 nm and 581 nm, respectively. This makes it a convenient excitation source for argon and krypton lasers. Although it was thought that the fluorescent signal of Rhod 2 only increases several times with the calcium complex, Dojindo’s Rhod 2 increases its signal with calcium 80-100 times because of its high purity....

Rhod 2-AM Rhod 2-AM, 1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane- N,N,N’,N’-tetraacetic acid, tetraacetoxymethyl ester, chloride [CAS: 129787-64-0]
Item #: R002 -

Product Description Rhod 2 has the longest fluorescent wavelength signal of all the calcium indicators. It has a rhodamine-like fluorophore whose excitation and emission maxima are 557 nm and 581 nm, respectively. This makes it a convenient excitation source for argon and krypton lasers. Although it was thought that the fluorescent signal of Rhod 2 temp-only increases several times with the calcium complex, Dojindo’s Rhod 2 increases its signal with calcium 80-100 times because of its high...

SPiDER-ßGal SPiDER-ßGal, SG02, SG02-10,
Item #: SG02 -

Product Description The gene of β-galactosidase from E. coli is widely used as a reporter gene assay marker. Although X-gal is well known reagent to detect β-galactosidase in cell or tissue samples, the assay using these reagents require to fix cells or tissues due to the poor cell-permeability. In addition, so far developed the assay using fluorescence reagents cannot clearly differentiate β-galactosidase-expressed cells or regions. To overcome these issues, Urano, Kamiya and co-workers have...

TPEN TPEN, N,N,N’,N’-Tetrakis(2-pyridylmethyl)ethylenediamine [CAS: 16858-02-9]
Item #: T040 -

Product Description TPEN is a highly selective chelator of transition metal cations because the pyridine groups act as soft electron donators. TPEN is thus utilized for masking transition metal cations in fluorescent calcium monitoring. The masking function of the zinc cation is important for canceling the Fluorescent effect. TPEN is water-soluble and cell membrane permeable. Maximum wavelength is 260 nm. The physical properties of TPEN are as follows: pKa1=10.27, pKa2=3.32, pKa3=4.85, and...

Zinquin ethyl ester Zinquin ethyl ester, Ethyl [[2-methyl-8-[[(4-methylphenyl)sulfonyl]amino]-6-quinolinyl]oxy]acetate [CAS: 151606-29-0]
Item #: Z215 -

Product Description Zinquin ethyl ester is an analog of the widely used indicator TSQ. Although Zinquin ethyl ester itself is fluorescent, its fluorescence intensity is negligibly weak (1/30). Zinquin ethyl ester is membrane permeable, as are acetoxymethyl esters of calcium probes such as Fura 2-AM and Fluo 3-AM. Zinquin ethyl ester is thus useful to detect intracellular zinc ions. It forms a complex with a zinc ion with nitrogen atoms in the structure. This compound also forms a fluorescent...