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BAPTA BAPTA, O,O’-Bis(2-aminophenyl)ethyleneglycol-N,N,N’,N’-tetraacetic acid, tetrapotassium salt, hydrate [CAS: 85233-19-8(free acid)]
Item #: B019 -

Product Description BAPTA is a calcium-selective chelator developed by Dr. Tsien. It has logKCa=6.97 and logKMg=1.77. The basic chelating unit resembles that of EGTA, but the two aliphatic nitrogen atoms are replaced by aromatic nitrogen. Thus, BAPTA is not protonated at physiological pH. BAPTA possesses pKa3=5.47 and pKa4=6.36. This property indicates that the deprotonation step is not included in its calcium complexation step, and it has a higher complexation rate than EGTA because it is not...

BAPTA-AM BAPTA-AM, O,O’-Bis(2-aminophenyl)ethyleneglycol-N,N,N’,N’-tetraacetic acid, tetraacetoxymethyl ester [CAS: 126150-97-8]
Item #: B018 -

Product Description BAPTA is a calcium-selective chelator developed by Dr. Tsien. It has logKCa=6.97 and logKMg=1.77. The basic chelating unit resembles that of EGTA, but the two aliphatic nitrogen atoms are replaced by aromatic nitrogen. Thus, BAPTA is not protonated at physiological pH. BAPTA possesses pKa3=5.47 and pKa4=6.36. This property indicates that the deprotonation step is not included in its calcium complexation step, and it has a higher complexation rate than EGTA because it is not...

BCECF BCECF, 2,7-Bis(carboxyethyl)-4 or 5-carboxyfluorescein [CAS: 85138-49-4]
Item #: B031 -

Product DescriptionBCECF is the most widely used intracellular pH probe. Dr. Tsien and others improved this carboxyfluorescein by introducing two extra carboxylates that allow it to be retained better by the cell. BCECF is highly water-soluble because it has 4 to 5 negative charges at neutral pH; it becomes difficult to pass through the cell membrane after loading. Its pKa value, 6.97, is higher than that of carboxyfluorescein. BCECF has an isosbestic point at 439 nm in the excitation spectra,...

Coelenterazine-WS Coelenterazine-WS, Coelenterazine, ß-cyclodextrin complex
Item #: C397 -

Product Description Aequorin is a luminescent protein that contains coelenterazine as a luminescent compound. Since aequorin emits luminescence by calcium conjugation, it is used for intracellular calcium ion detection. However, coelenterazine has poor water solubility under physiological conditions, and it is adsorbed to cell membranes. Dojindo’s Coelenterazine-WS is a β-cyclodextrin complex of coelenterazine with drastically improved water solubility at neutral pH. Chemical Structure ...

DiBAC4(3) DiBAC4(3), Bis(1,3-dibutylbarbituric acid)trimethine oxonol, sodium salt
Item #: D545 -

Product Description DiBAC4(3) is a Bis-oxonol-type, membrane-potential, sensitive dye. According to membrane depolarization detection, when DiBAC distribution in cytosol is increased, the fluorescence intensitiy is increased. Since an argon laser (488 nm) can be used for DiBAC4(3) excitation, it is applicable for flow cytometry and confocal microscopy. Chemical Structure ...

FerroOrange FerroOrange, F374, F374-10, F374-12
Item #: F374 -

Product Description Iron is the most abundant transition metal element within organisms, and it participates in various physiological activities. Recently, free iron in living cells has attracted attention because its high reactivity may be related to cellular damage or death. Free iron exists in its stable redox states, namely ferrous ion (Fe2+) and ferric ion (Fe3+). In living cells, understanding the behavior of Fe2+is considered more important than understanding that of Fe3+ because of the...

Fluo 3 Fluo 3, 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N’,N’-tetraacetic acid [CAS: 123632-39-3]
Item #: F019 -

Product Description Fluo 3 is a long wavelength calcium probe that is practically nonfluorescent in its free ligand form, but its fluorescence increases 60-80 times when it forms complexes with calcium. Thus, it has been widely used with confocal laser fluorescent microscopy because the microscope has an argon laser. The long wavelength of the fluorescent signal is also convenient for minimizing photodamage to sample cells. Fluo 3 is also useful for caged calcium and others that are cleaved by...

Fluo 3-AM Fluo 3-AM, 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N’, N’-tetraacetic acid, pentaacetoxymethyl ester [CAS: 121714-22-5]
Item #: F023 -

Product Description Fluo 3 is a long wavelength calcium probe that is practically nonfluorescent in its free ligand form, but its fluorescence increases 60-80 times when it forms complexes with calcium. Thus, it has been widely used with confocal laser fluorescent microscopy because the microscope has an argon laser. The long wavelength of the fluorescent signal is also convenient for minimizing photodamage to sample cells. Fluo 3 is also useful for caged calcium and others that are cleaved by...

Fura 2 Fura 2, 1-[6-Amino-2-(5-carboxy-2-oxazolyl)-5-benzofuranyloxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N’,N’- tetraacetic acid, pentapotassium salt [CAS: 96314-98-6]
Item #: F014 -

Product Description Fura 2 was developed to improve the fluorescent properties of Quin 2. The signal intensity in 1 mM of loaded Fura 2 corresponds to that of 30 mM of loaded Quin 2. This allows an experiment at a lower concentration of indicator using Fura 2 as compared to Quin 2. Fura 2 is one of the most widely used calcium indicators for ratiometric measurement. Many types of instrumentation are now available for experiments using Fura 2. Fura 2 is especially suitable for digital imaging...

Fura 2-AM Fura 2-AM, 1-[6-Amino-2-(5-carboxy-2-oxazolyl)-5-benzofuranyloxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N’,N’- tetraacetic acid, pentaacetoxymethyl ester [CAS: 108964-32-5]
Item #: F015 -

Product Description Fura 2 was developed to improve the fluorescent properties of Quin 2. The signal intensity in 1 mM of loaded Fura 2 corresponds to that of 30 mM of loaded Quin 2. This allows an experiment at a lower concentration of indicator using Fura 2 instead of Quin 2. Fura 2 is one of the most widely used calcium indicators for ratiometric measurement. Many types of instrumentation are now available for experiments using Fura 2, but Fura 2 is especially suitable for digital imaging...

Glucose Uptake Assay Kit-Blue
Item #: UP01 -

Detection Principle Nutrient metabolism is necessary for energy production in cells and regulates various cellular functions, including gene expression. Glucose is one of the key substrates for the generation of ATP and to sustain cellular homeostasis. Thus, glucose metabolism has been the subject of intense investigations. In cancer research, tumor cells enhance glucose uptake and consumption for their growth and proliferation. Therefore, elevated glucose uptake is a marker of tumors, and...

Glucose Uptake Assay Kit-Red
Item #: UP03 -

Detection Principle Nutrient metabolism is necessary for energy production in cells and regulates various cellular functions, including gene expression. Glucose is one of the key substrates for the generation of ATP and to sustain cellular homeostasis. Thus, glucose metabolism has been the subject of intense investigations. In cancer research, tumor cells enhance glucose uptake and consumption for their growth and proliferation. Therefore, elevated glucose uptake is a marker of tumors, and...

Lipi-Series: Lipid Droplet Detection Probes(LD01, LD02, LD03, LD04) LD01, LD01-10, Lipi-Blue, Lipid Droplet
Item #: LD01 -

LD01 LD02 LD03 LD04 Product Description Lipid droplets (LDs) are composed of neutral lipids such as triacylglycerol & cholesteryl ester that are surrounded by phospholipid monolayers and are seen ubiquitously, not only in adipocytes1). LDs were originally thought to serve as a lipid storage unit, until a recent study showing that LDs play an important role in regulating lipid metabolism, autophagy2) and cellular senescence3). Therefore, LDs have gained great attention as an important tool to...

Lipid Droplet Assay Kit-Blue Lipid Droplet, LD05, LD05-10, Lipid Droplet Assay Kit-Blue
Item #: LD05 -

Product Description Lipi probes are small molecule which emit strong fluorescence in hydrophobic environment such as in LDs. Function of lipid droplets   Lipid droplets (LDs) are composed of neutral lipids such as triacylglycerol & cholesteryl ester that are surrounded by phospholipid monolayers and are seen ubiquitously, not only in adipocytes1). Although LDs were simply thought to serve as a lipid storage unit, a recent study has stated that LDs play an important role in regulating...

Lipid Droplet Assay Kit-Deep Red Lipid Droplet, LD06, LD06-10, Lipid Droplet Assay Kit-Deep Red
Item #: LD06 -

Product Description Lipi probes are small molecule which emit strong fluorescence in hydrophobic environment such as in LDs. Function of lipid droplets   Lipid droplets (LDs) are composed of neutral lipids such as triacylglycerol & cholesteryl ester that are surrounded by phospholipid monolayers and are seen ubiquitously, not only in adipocytes1). Although LDs were simply thought to serve as a lipid storage unit, a recent study has stated that LDs play an important role in regulating...

MQAE MQAE, N-Ethoxycarbonylmethyl-6-methoxyquinolinium bromide [CAS: 124505-60-8]
Item #: M024 -

Product Description MQAE with bromide ion as a counteranion has fluorescence excitation at 355 nm and emission at 460 nm. The fluorescent intensity of MQAE decreases in proportion to the chloride increase in cells. Chloride ion concentrations less than 50 mM may be monitored without being affected by pH changes. MQAE has high water solubility and membrane permeability. Chemical Structure ...

Nucleolus Bright Green Nucleolus staining, N511, N512
Item #: N511 -

Detection Principle A Nucleolus is one of the structures forming a nucleus and becoming the starting point of the ribosomal biogenesis. There are many ribosomal RNA (rRNA) in the nucleolus and transcription of rRNA and processing are carried out. Whereas the morphological change of nucleolus is known as one of the indicator of cancer diagnosis, there has been some scientific reports describing the relation between nucleolus and DNA damge, autophagy, virus infection, and cellular senescence....

Nucleolus Bright Red Nucleolus staining, N511, N512
Item #: N512 -

Detection Principle A Nucleolus is one of the structures forming a nucleus and becoming the starting point of the ribosomal biogenesis. There are many ribosomal RNA (rRNA) in the nucleolus and transcription of rRNA and processing are carried out. Whereas the morphological change of nucleolus is known as one of the indicator of cancer diagnosis, there has been some scientific reports describing the relation between nucleolus and DNA damge, autophagy, virus infection, and cellular senescence....

Quin 2 Quin 2, 8-Amino-2-[(2-amino-5-methylphenoxy)methyl]-6-methoxyquinoline-N,N,N’,N’-tetraacetic acid, tetrapotassium salt [CAS: 73630-23-6]
Item #: Q001 -

Product Description Quin 2 forms a stable fluorescent complex with calcium (logKCaY=7.1), but not with magnesium (logMgY=2.7). The complex has a high quantum yield (0.14) at emission wavelength 525 nm, excitation wavelength 339 nm, and emission wavelength 492 nm. Quin 2-AM is an acetoxymethyl ester derivative of Quin 2 that is readily permeable through cell membranes. Within the cell, the ester is hydrolyzed to Quin 2. Calcium Chelation ...

Rhod 2-AM Rhod 2-AM, 1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane- N,N,N’,N’-tetraacetic acid, tetraacetoxymethyl ester, chloride [CAS: 129787-64-0]
Item #: R002 -

Product Description Rhod 2 has the longest fluorescent wavelength signal of all the calcium indicators. It has a rhodamine-like fluorophore whose excitation and emission maxima are 557 nm and 581 nm, respectively. This makes it a convenient excitation source for argon and krypton lasers. Although it was thought that the fluorescent signal of Rhod 2 temp-only increases several times with the calcium complex, Dojindo’s Rhod 2 increases its signal with calcium 80-100 times because of its high...

TPEN TPEN, N,N,N’,N’-Tetrakis(2-pyridylmethyl)ethylenediamine [CAS: 16858-02-9]
Item #: T040 -

Product Description TPEN is a highly selective chelator of transition metal cations because the pyridine groups act as soft electron donators. TPEN is thus utilized for masking transition metal cations in fluorescent calcium monitoring. The masking function of the zinc cation is important for canceling the Fluorescent effect. TPEN is water-soluble and cell membrane permeable. Maximum wavelength is 260 nm. The physical properties of TPEN are as follows: pKa1=10.27, pKa2=3.32, pKa3=4.85, and...

Zinquin ethyl ester Zinquin ethyl ester, Ethyl [[2-methyl-8-[[(4-methylphenyl)sulfonyl]amino]-6-quinolinyl]oxy]acetate [CAS: 151606-29-0]
Item #: Z215 -

Product Description Zinquin ethyl ester is an analog of the widely used indicator TSQ. Although Zinquin ethyl ester itself is fluorescent, its fluorescence intensity is negligibly weak (1/30). Zinquin ethyl ester is membrane permeable, as are acetoxymethyl esters of calcium probes such as Fura 2-AM and Fluo 3-AM. Zinquin ethyl ester is thus useful to detect intracellular zinc ions. It forms a complex with a zinc ion with nitrogen atoms in the structure. This compound also forms a fluorescent...