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2,3-Diaminonaphthalene (for NO detection) 2,3-Diaminonaphthalene (for NO detection), 2,3-Diaminonaphthalene [CAS: 771-97-1]
Item #: D418 -

Product Description The Griess assay is a simple and popular method for detecting NO concentration. 2,3-Diaminonaphthalene (DAN) is a highly sensitive alternative to the Griess assay. The DAN method is 50-100 times more sensitive than the Griess assay: While the detection limit of the Griess assay is 1 mM, the limit of the DAN method is 10-50 nM. DAN reacts with NO2- in acidic conditions to produce fluorescent naphthalenetriazole. The wavelength of the emission maximum of naphthalenetriazole...

2-PDS 2-PDS, 2,2’-Dithiodipyridine [CAS: 2127-03-9]
Item #: P016 -

Product Description 2-PDS and 4-PDS are used for the photometric determination of thiol groups in biological samples. These compounds react with the thiol groups at pH 5 to form mercaptopyridine. The maximum wavelengths of 2-Mercaptopyridine and 4-Mercaptopyridine are 343 nm and 324 nm, respectively. ...

3-Deoxyglucosone 3-Deoxyglucosone, 3-Deoxy-D-erythro-hexos-2-ulose [CAS: 4084-27-9]
Item #: D535 -

Product Description Advanced glycation end-products (AGEs) have been studied as one of the causes of diabetic complications. Several compounds have been identified as AGEs, including pyralline, pentosidine, imidazolone, and pyropyridine. Glyoxal and methylglyoxal are reactive dicarbonyl compounds generated by glucose self-oxidation that are known to be AGE precursors. Another dicarbonyl compound, 3-Deoxyglucosone (3-DG), is also known to be one of the AGE precursors. 3-DG is derived from the...

4-PDS 4-PDS, 4,4’-Dithiodipyridine [CAS: 2645-22-9]
Item #: P017 -

Product Description 2-PDS and 4-PDS are used for the photometric determination of thiol groups in biological samples. These compounds react with the thiol groups at pH 5 to form mercaptopyridine. The maximum wavelengths of 2-Mercaptopyridine and 4-Mercaptopyridine are 343 nm and 324 nm, respectively. ...

BMPO BMPO, 5-tert-Butoxycarbonyl-5-methyl-1-pyrroline-N-oxide
Item #: B568 -

Chemical Structure Product Description Spin trapping analysis is one of the most reliable techniques for detecting and identifying short-lived free radicals. The EPR (ESR) spin trap reagent detects both superoxide and hydroxyl radicals produced by systems in vitro and in vivo.  BMPO was developed as a spin trapping reagent that adducts superoxide and shows a much longer half-life (t1/2=23 min) than other spin trap reagents. It gives us reproducible and steady results. Because BMPO is...

Carboxy-PTIO Carboxy-PTIO, 2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, sodium salt [CAS: 148819-93-6]
Item #: C348 -

Product DescriptionCarboxy-PTIO is a stable, water-soluble organic radical that reacts with NO to form NO2· This reaction can be monitored by electron spin resonance (ESR). NO is an unstable molecule and has a complex reaction cascade for its metabolism in biological systems. Rapidly generated NO-related metabolites carry out various physiological activities. Commonly used NO scavengers such as hemoglobin trap NO; they also trap NOS inhibitors such as arginine derivatives. These NO scavengers...

DMPO DMPO, Purity, High Purity, 3317-61-1
Item #: D048 -

Product Description Because of potential cancer risks and their age-promoting effects, free radicals in living bodies have become a frequently studied subject. DMPO is the most frequently used spin-trapping reagent for the study of free radicals. It is suitable for trapping oxygen radicals, especially superoxides, and for producing adducts with characteristic EPR (ESR) patterns. However, most commercially available DMPO contains impurities that cause high backgrounds. Thus, DMPO requires...

DNA Damage Quantification Kit-AP Site Counting- DNA Damage Quantification Kit-AP Site Counting-, ROS, Oxdative stress, Comet assay,  Genomic DNA, 8-OHdG, Abasic site, Hydroxy radical, Aldehyde reactive probe, ARP, DNA repair
Item #: DK02 -

Product Description Oxidative damage to DNA is a result of its interaction with reactive oxygen species (ROS), in particular, the hydroxy radical. Hydroxy radicals, which are produced from superoxide anion and hydrogen peroxide by the Fenton reaction, produce multiple modifications in DNA. Oxidative attacks by hydroxy radicals on the deoxyribose moiety will lead to the release of free bases from DNA, generating strand breaks with various sugar modifications and simple abasic sites (AP sites)....

DPPH Antioxidant Assay Kit DPPH Antioxidant Assay Kit, Antioxidant, D678
Item #: D678 -

Product Description Changes in antioxidant capacity of the body are involved in the development of various diseases and health problems. As a result, this increases expectations for foods with antioxidant activity (antioxidant foods). Kochi University's Shimamura et al. have developed a method using DPPH (2,2-Diphenyl-1-picrylhydrazyl) for evaluating antioxidant activity with little difference between measurement facilities1). This product uses a method including a microplate based on the...

DPPP DPPP, Diphenyl-1-pyrenylphosphine [CAS: 110954-36-4]
Item #: D350 -

Product Description DPPP is a non-fluorescent triphenylphosphine compound. It reacts with hydroperoxide to generate DPPP oxide that emits fluorescence at 352 nm excitation and 380 nm emission wavelengths (Fig. 1). Post-column HPLC method is used to determine phospholipid peroxide in sample solutions. Fig 1. DPPP reaction with lipid peroxidase ...

DTCS Na DTCS Na, N-(Dithiocarboxy)sarcosine, disodium salt, dihydrate [CAS: 13442-87-0]
Item #: D465 -

Product Description Diethyldithiocarbamate (DETC) is a good spin-trapping reagent for nitric oxide in vivo. However, DETC has not been widely utilized for NO detection in biological samples due to its poor water solubility. DTCS, an analog of DETC, forms a water-soluble iron(II) complex (Fe-DTCS). The Fe-DTCS complex then forms a complex with NO (NO-Fe-DTCS). Dr. Yoshimura successfully obtained two-dimensional ESR images of NO, induced by lipopolysaccharide in mouse peritoneum. DTCS sodium...

DTNB DTNB, 5,5’-Dithiobis(2-nitrobenzoic acid) [CAS: 69-78-3]
Item #: D029 -

Product Description DTNB is known as Ellman’s reagent. It is used for the colorimetric determination of thiol groups in biological samples. It is fairly soluble in water. Colorless DTNB is converted to yellow 5-Mercapto-2- nitrobenzoic acid in the presence of thiol compounds (Fig. 1). As 5-Mercapto-2-nitrobenzoic acid has an absorption maximum at 412 nm, the absorption spectrum of DTNB does not interfere with thiol detection (Fig. 2). Fig. 1 DTNB reaction with thiol compound Fig. 2 Absorption...

GSSG/GSH Quantification Kit Glutathione,  GSSG/GSH Quantification Kit
Item #: G257 -

Product DescriptionGlutathione (γ-L-glutamyl-L-cysteinylglycine) is a tripeptide present in the body, and it is involved in antioxidation, drug metabolism, and other as enzyme substrate of glutathione peroxidase, glutathione S-transferase, and thiol transferase, etc. Glutathione is usually present as reduced form (GSH), but GSH is converted into its oxidized form (GSSG) by stimulation such as oxidative stress. Therefore, the ratio of GSH and GSSG has been noted as index of oxidative stress.The...

Liperfluo Lipid peroxide imaging is available by a fluorescence microscopy and a flow cytometric analysis for living cells.
Item #: L248 -

Product Description Liperfluo, a perylene derivative containing oligooxyethylene, is designed and exclusively developed by Dojindo for the detection of lipid peroxides. Liperfluo emits intense fluorescence by lipid peroxide specific oxidation in organic solvents such as ethanol. Among fluorescent probes that detect Reactive Oxygen Species(ROS), Liperfluo is the only compound that can specifically detect lipid peroxides. Since the excitation and emission wavelengths of the oxidized Liperfluo...

MitoPeDPP autophagy, mitochondria, Lipid peroxide, Lipid peroxidation, ROS imaging, TBA, TBARS
Item #: M466 -

Product Description MitoPeDPP is a cell-membrane-permeable probe, Perylene-based dye. It specifically localizes in mitochondria due to the triphenylphosphonium moiety introduced. MitoPeDPP is used to monitor lipid peroxidation in ferroptosis research. As the excitation and emission wavelength of MitoPeDPP are 452 nm and 470 nm, respectively, the probe can be applied for lipophilic peroxide imaging in living cells. *This probe has been developed by Dr. Shioji et al. at Fukuoka University,...

NOC 12 NOC 12, 1-Hydroxy-2-oxo-3-(N-ethyl-2-aminoethyl)-3-ethyl-1-triazene [CAS: 146724-89-2]
Item #: N378 -

Reaction of NO release Product Description of NOC Compounds NOCs are stable NO-amine complexes that spontaneously release NO, without cofactors, under physiological conditions. The rate of NO release depends on the chemical structure of NOC. The mechanism of spontaneous NO generation by NOCs is very simple compared to other classical NO donors, such as nitroglycerin and nitropurusside, and the by-products do not interfere with cell activities. A single NOC molecule releases two NO molecules...

NOC 18 NOC 18, 1-Hydroxy-2-oxo-3,3-bis(2-aminoethyl)-1-triazene [CAS: 146724-94-9]
Item #: N379 -

Reaction of NO release Product Description of NOC Compounds are stable NO-amine complexes that spontaneously release NO, without cofactors, under physiological conditions. The rate of NO release depends on the chemical structure of NOC. The mechanism of spontaneous NO generation by NOCs is very simple compared to other classical NO donors, such as nitroglycerin and nitropurusside, and the by-products do not interfere with cell activities. A single NOC molecule releases two NO molecules (as...

NOC 5 NOC 5, 1-Hydroxy-2-oxo-3-(3-aminopropyl)-3-isopropyl-1-triazene [CAS: 146724-82-5]
Item #: N380 -

Reaction of NO release Product Description of NOC CompoundsNOCs are stable NO-amine complexes that spontaneously release NO, without cofactors, under physiological conditions. The rate of NO release depends on the chemical structure of NOC. The mechanism of spontaneous NO generation by NOCs is very simple compared to other classical NO donors, such as nitroglycerin and nitropurusside, and the by-products do not interfere with cell activities. A single NOC molecule releases two NO molecules (as...

NOC 7 NOC 7, 1-Hydroxy-2-oxo-3-(N-methyl-3-aminopropyl)-3-methyl-1-triazene [CAS: 146724-84-7]
Item #: N377 -

Reaction of NO release Product Description of NOC Compounds NOCs are stable NO-amine complexes that spontaneously release NO, without cofactors, under physiological conditions. The rate of NO release depends on the chemical structure of NOC. The mechanism of spontaneous NO generation by NOCs is very simple compared to other classical NO donors, such as nitroglycerin and nitropurusside, and the by-products do not interfere with cell activities. A single NOC molecule releases two NO molecules...

NOR 3 NOR 3, (+)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide [CAS: 163180-49-2]
Item #: N390 -

Reaction of NO release Product Description of NOR Compounds NORs are ideal NO donors with completely different chemical structures from the other NO donors. Although NORs do not have any ONO2 or ONO moiety, they spontaneously release NO at a steady rate. Even though the NO release mechanism of NOR has not been completely determined, it is confirmed that the byproducts do not possess any significant bioactivities. NOR 3, isolated from Streptomyces genseosporeus, is reported to have strong...

ROS Assay Kit -Highly Sensitive DCFH-DA-
Item #: R252 -

Reactive oxygen species (ROS) are primarily produced in mitochondria during ATP synthesis. ROS play an essential role in signaling pathways and the immune system, whereas increasing ROS is associated with diseases and cellular senescence. Recent studies suggested that ferroptosis is a new type of cell death characterized by iron dependency and increasing ROS. Thus, ROS detection has been attracting considerable interest in ferroptosis research. DCFH-DA is widely used for ROS detection, but it...

S-Nitrosoglutathione S-Nitrosoglutathione, N-(N-L-?-Glutamyl-S-nitroso-L-cysteinyl)glycine [CAS: 57564-91-7]
Item #: N415 -

Reaction of NO release Product Description of S-Nitrosothiols Nitrosothiol compounds release NO and become disulfides under specific physiological conditions. While most of the S-nitrosothiol compounds are unstable, S-Nitrosoglutathione is exceptionally stable. Furthermore, S-Nitrosoglutathione is water-soluble. Although S-nitrosothiol is a good NO donor with no nitrate tolerance, there is evidence that S-nitrosothiol itself has NO-like activity during guanylate cyclase activation. Another...

Si-DMA for Mitochondrial Singlet Oxygen Imaging MT05, MT05-10, Si-DMA for Mitochondrial Singlet Oxygen Imaging,
Item #: MT05 -

Product Description Singlet oxygen (1O2) is one of the Reactive Oxygen Species (ROS). 1O2 is known to be a cause of spots and wrinkles of the skin due to its very strong oxidizing potential. In the field of cancer research, 1O2 is of a particular importance because of its key role in photodynamic therapy (PDT), an emerging anticancer treatment using photoirradiation and photosensitizers. For the detection of 1O2, the existing singlet oxygen fluorescence detection reagent cannot be used in...

SIN-1 SIN-1, 3-(4-Morpholinyl)sydnonimine, hydrochloride [CAS: 16142-27-1]
Item #: S264 -

Product Description SIN-1, a metabolite of the vasodilator molsidomine, is utilized to separately estimate the effectiveness of NO and peroxynitrite with other NO donors. SIN-1 spontaneously decomposes in the presence of molecular oxygen to generate NO and superoxide. Both products bind very rapidly to form peroxynitrite (rate constant k: 3.7x107 M-1s-1). Therefore, SIN-1 is a useful compound that generates peroxynitrite in an efficient manner. Peroxynitrite is a very strong oxidant that...

SOD Assay Kit-WST SOD Assay Kit-WST, Superoxide dismutase, ROS, Oxidative stress, Superoxide anion, Radical, NBT, NBT method, SOD1, SOD2, WST-1, IC50, Cu/Zn-SOD, Xanthine, Xanthine oxidase, SOD inhibition activity, Superoxid, NBT
Item #: S311 -

Product Description Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion (O2.-) into hydrogen peroxide and molecular oxygen, is one of the most important antioxidative enzymes. In mammals, cytosolic SOD has a greenish color and consists of two subunits, one containing copper and the other zinc (Cu/Zn-SOD). Mitochondrial and bacterial SOD has a reddish-purple color and contains manganese (Mn-SOD). E. coli has Mn-SOD and SOD containing iron (Fe-SOD). Several direct...

Spy-LHP Spy-LHP, 2-(4-Diphenylphosphanyl-phenyl)-9-(1-hexyl-heptyl)-anthra[2,1,9-def,6,5,10-d’e’f’]diisoquinoline- 1,3,8,10-tetraone
Item #: S343 -

Product Description Spy-LHP is a newly developed fluorescent probe for live cell imaging of phospholipid peroxide. There are several detection methods available for lipid peroxides, such as iodide titration method, colorimetric method, or chemiluminometric method to determine malondialdehyde or 4-hydroxynonenal. Malondialdehyde and 4-hydroxynonenal are derivatives from lipid hydroperoxide prepared by oxidation with reactive oxygen species. Thiobarbituriic acid and 1-Methyl-2-phenylindole are...

Total Glutathione Quantification Kit Total Glutathione Quantification Kit
Item #: T419 -

Product Description Glutathione (GSH) is the most abundant thiol compound in animal tissues, plant tissues, bacteria, and yeast. GSH has many different roles, including protection against reactive oxygen species and the maintenance of protein thiol groups. During these processes, GSH is converted into its oxidized form, glutathione disulfide (GSSG). Since GSSG is then enzymatically reduced by glutathione reductase, GSH is the dominant form in organisms. DTNB (5,5 EDithiobis(2-nitrobenzoic...