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Ab-10 Rapid Biotin Labeling Kit
Item #: LK37 -

Product Description Ab-10 Rapid Biotin Labeling Kit enables rapid (in less than 30 min) and easy labeling of Biotin to 10 μg antibody. Reactive Biotin (a component of the kit) has succinimidyl ester group, that can easily make a covalent bond with an amino group of the target antibody without any activation process. This kit contains all the necessary reagents to prepare a Biotin-labeled antibody except for DMSO. Fig. 1 Labeling Procedure Precaution ♦ Use 0.5-1 mg/ml of antibody solution for...

Ab-10 Rapid Fluorescein Labeling Kit
Item #: LK32 -

Product Description Ab-10 Rapid Fluorescein Labeling Kit is for labeling fluorescein to 10 µg antibody in less than 30 minutes. Labeling agent included in the kit has succinimidyl ester group , which can easily make a covalent bond with an amino group of the target antibody without any activation process. The kit contains all the necessary reagents for antibody labeling. Fig. 1 IgG Labeling Reaction of NH2-reactive Fluorescein Fig. 2 Labeing Procedure Fig. 3 Fluorescence Spectrum of...

Ab-10 Rapid HiLyte Fluor 555 Labeling Kit
Item #: LK35 -

Product Description Ab-10 Rapid HiLyte Fluor* 555 Labeling Kit enables rapid (in less than 30 min) and easy labeling of HiLyte Fluor 555 to 10 μg antibody. Reactive HiLyte Fluor 555 (a component of the kit) has succinimidyl ester group, that can easily make a covalent bond with an amino group of the target antibody without any activation process. This kit contains all the necessary reagents to prepare a fluorescein-labeled antibody. *HiLyte Fluor is a trademark of AnaSpec, Inc. Fig. 1 Labeing...

Ab-10 Rapid HiLyte Fluor 647 Labeling Kit
Item #: LK36 -

Product Description Ab-10 Rapid HiLyte Fluor* 647 Labeling Kit enables rapid (in less than 30 min) and easy labeling of HiLyte Fluor 647 to 10 μg antibody. Reactive HiLyte Fluor 647 (a component of the kit) has succinimidyl ester group, that can easily make a covalent bond with an amino group of the target antibody without any activation process. This kit contains all the necessary reagents to prepare a fluorescein-labeled antibody. *HiLyte Fluor is a trademark of AnaSpec, Inc. Fig. 1 Labeing...

Ab-10 Rapid Peroxidase Labeling Kit
Item #: LK33 -

Product Description Ab-10 Rapid Peroxidase Labeling Kit is for labeling peroxidase to 10 µg antibody in less than 30 minutes. Labeling agent included in the kit has succinimidyl ester group , which can easily make a covalent bond with an amino group of the target antibody without any activation process. The kit contains all the necessary reagents for antibody labeling. Fig. 1 IgG Labeling Reaction of NH2-reactive Peroxidase Fig. 2 Labeing Procedure Precaution ♦ Use 0.5-1 mg/ml of antibody...

Ab-10 Rapid R-Phycoerythrin Labeling Kit
Item #: LK34 -

Product Description Ab-10 Rapid R-Phycoerythrin Labeling Kit is for labeling R-Phycoerythrin to 10 µg antibody in less than 30 minutes. Labeling agent included in the kit has succinimidyl ester group , which can easily make a covalent bond with an amino group of the target antibody without any activation process. The kit contains all the necessary reagents for antibody labeling. Fig. 1 IgG Labeling Reaction of NH2-reactive R-Phycoerythrin Fig. 2 Labeing Procedure Fig. 3 Fluorescence Spectrum...

AB-NTA free acid AB-NTA free acid, N-(5-Amino-1-carboxypentyl)iminodiacetic acid [CAS: 129179-17-5]
Item #: A459 -

Product Description Aminobutyl-NTA (AB-NTA) free acid replaces AB-NTA (disodium salt form; former Product Code: A296-10). The new free acid form is less hygroscopic than the disodium salt form. Dissolving the new acid form in a buffer involves the same procedure as dissolving disodium salt in a buffer. The new acid form can also be dissolved in water with sonication (up to 3%). Aminobutyl-NTA was first used by Dr. Hochuli in 1987 to purify recombinant proteins (the “Histag” technique). Since...

ABD-F ABD-F, 4-Fluoro-7-sulfamoylbenzofurazan [CAS: 91366-65-3]
Item #: A016 -

Derivatization Reaction Product Description ABD-F has a benzofurazan moiety that produces a highly fluorescent compound through the reaction with a sulfhydryl group. The excitation and emission of the devivatized compound are 389 nm and 513 nm, respectively. The reaction rate of ABD-F is 30 times faster than that of SBD-F. ABD-F reactions with thiol compounds are completed within 5 minutes in aqueous conditions at 50ºC, pH 8. However, ABD-F does not react with alanine, proline, or cystine...

Alkaline Phosphatase Labeling Kit-NH2
Item #: LK12 -

Product Description Alkaline Phosphatase Labeling Kit-NH2 is mainly used for the preparation of alkaline phosphatase-labeled IgG for enzyme immunoassay (EIA) and for the preparation of alkaline phosphataselabeled antigen for competitive EIA. NH2-reactive ALP, a component of this kit, can react with amino groups of proteins or other molecules (Fig. 1-15). This kit contains all of the  necessary reagents for the labeling process including Storage buffer. NH2-reactive ALP forms a covalent...

Alkaline Phosphatase Labeling Kit-SH
Item #: LK13 -

Product Description Alkaline Phosphatase Labeling Kit-SH is used mainly for the preparation of alkaline phosphatase-labeled IgG for enzyme immunoassay (EIA) and for the preparation of alkaline phosphatase-labeled antigen for competitive EIA. SH-reactive ALP, a component of this kit, can react with the thiol groups of proteins or other molecules (Fig.1). This kit contains all the reagents necessary for the labeling process, including reducing agent and storage buffer. SH-reactive ALP forms a...

Allophycocyanin Labeling Kit-NH2
Item #: LK21 -

Product Description Phycobiliproteins are fluorescent proteins derived from cyanobacteria and eukaryotic algae. Their fluorescence is much higher than chemical fluorescent probes such as fluorescein and rhodamine. Allophycocyanin (APC) is one of the phycobiliproteins, and it has a red fluorescence at around 660 nm (Fig. 1). Because of this high fluorescence, phycobiliprotein-labeled antibodies and other molecules can provide greater sensitivity in flow cytometry and immunostaining....

Allophycocyanin Labeling Kit-SH
Item #: LK24 -

Product Description Phycobiliproteins are fluorescent proteins derived from cyanobacteria and eukaryotic algae. Their fluorescence is much higher than that of chemical fluorescent probes such as fluorescein and rhodamine. Allophycocyanin (APC) is one of the phycobiliproteins, and it has a red fluorescence at around 660 nm (Fig. 1). Because of this high fluorescence, phycobiliprotein-labeled antibodies and other molecules can provide greater sensitivity in flow cytometry and immunostaining....

ARP (Aldehyde Reactive Probe) ARP (Aldehyde Reactive Probe), N-(Aminooxyacetyl)-N’-biotinyl-hydrazine [CAS: 139585-03-8]
Item #: A305 -

Product DescriptionAldehyde reactive probe (ARP) is used to detect abasic sites (AP sites, depurine/depyrimidine sites) in DNA because of its aldehyde-specific reactivity. ARP reacts with aldehyde and ketone groups, and it adds biotin to AP sites in DNA. The biotin-tagged DNA is then detected using peroxidase-labeled avidin and oxidative chromogenic dyes. The ARP method has been used to detect less than 1 AP site in 1x104 nucleotides of DNA. Dojindo offers a DNA Damage Quantification Kit...

BABE BABE, 1-(p-Bromoacetamidobenzyl) ethylenediamine N,N,N’,N’-tetraaceticacid [CAS: 81677-64-7]
Item #: B437 -

Product Description of BABEs Bromoacetamidobenzyl-EDTA (BABE) is a chelate labeling reagent that conjugates with sulfhydryl groups. The iron chelate of BABE (FeBABE) is a unique tool for determining the three-dimensional structure of proteins and the binding structures of protein-protein or protein-DNA complexes. BABE adds EDTA moieties to proteins through their sulfhydryl groups. Once attached to a protein, FeBABE cuts a nearby peptide or DNA chain. The cleavage site is within 12 angstroms of...

Biotin Labeling Kit-NH2
Item #: LK03 -

LK03-10 LK55-10 Product Description Biotin Labeling Kit-NH2 is mainly used for the preparation of biotin-labeled IgG for enzyme immunoassay (EIA). NH2-reactive biotin, a component of this kit, has succinimidyl groups (NHS) that react with amino groups on proteins or other molecules (Fig. 1). This kit contains all the reagents necessary for the labeling. The labeling process is very simple. Just add the NH2- reactive Biotin to IgG solution and incubate at 37oC for 10 minutes. An average of 5 to...

Biotin Sulfo-OSu Biotin Sulfo-OSu, Biotin N-hydroxy-sulfosuccinimide ester [CAS: 119616-38-5]
Item #: B319 -

Product Description of Amine-Reactive BiotinsThe avidin-biotin system has many applications in immunology and histochemistry. The interaction between avidin and biotin is remarkably strong with a dissociation constant on the order of 10-15 M. Biotin is usually added to primary or secondary antibodies such as anti-IgG and anti-IgM. After preparing the antigen-antibody complex with the biotin-labeled antibody, colorimetric, or fluorometric detection of the antigen is performed using enzyme or...

Biotin-(AC5)2 Sulfo-OSu Biotin-(AC5)2 Sulfo-OSu, 6-[6-(Biotinylamino)hexanoylamino]hexanoic acid N-hydroxysulfosuccinimide ester [CAS: 180028-78-8(free acid)]
Item #: B321 -

Product Description of Amine-Reactive Biotins The avidin-biotin system has many applications in immunology and histochemistry. The interaction between avidin and biotin is remarkably strong with a dissociation constant on the order of 10-15 M. Biotin is usually added to primary or secondary antibodies such as anti-IgG and anti-IgM. After preparing the antigen-antibody complex with the biotin-labeled antibody, colorimetric, or fluorometric detection of the antigen is performed using enzyme or...

Biotin-(AC5)2-OSu 6-[6-(Biotinylamino)hexanoylamino]hexanoic acid N-hydroxysuccinimide ester [CAS: 89889-52-1]
Item #: B306 -

Product Description of Amine-Reactive Biotins The avidin-biotin system has many applications in immunology and histochemistry. The interaction between avidin and biotin is remarkably strong with a dissociation constant on the order of 10-15 M. Biotin is usually added to primary or secondary antibodies such as anti-IgG and anti-IgM. After preparing the antigen-antibody complex with the biotin-labeled antibody, colorimetric, or fluorometric detection of the antigen is performed using enzyme or...

Biotin-AC5 Sulfo-OSu Biotin-AC5 Sulfo-OSu, 6-(Biotinylamino)hexanoic acid N-hydroxy-sulfosuccinimide ester[CAS: 109940-19-4]
Item #: B320 -

Product Description of Amine-Reactive Biotins The avidin-biotin system has many applications in immunology and histochemistry. The interaction between avidin and biotin is remarkably strong with a dissociation constant on the order of 10-15 M. Biotin is usually added to primary or secondary antibodies such as anti-IgG and anti-IgM. After preparing the antigen-antibody complex with the biotin-labeled antibody, colorimetric, or fluorometric detection of the antigen is performed using enzyme or...

Biotin-AC5-OSu 6-(Biotinylamino)hexanoic acid N-hydroxysuccinimide ester [CAS: 72040-63-2]
Item #: B305 -

Product Description of Amine-Reactive Biotins The avidin-biotin system has many applications in immunology and histochemistry. The interaction between avidin and biotin is remarkably strong with a dissociation constant on the order of 10-15 M. Biotin is usually added to primary or secondary antibodies such as anti-IgG and anti-IgM. After preparing the antigen-antibody complex with the biotin-labeled antibody, colorimetric, or fluorometric detection of the antigen is performed using enzyme or...

Biotin-OSu Biotin-OSu, Biotin N-hydroxysuccinimide ester [CAS: 35013-72-0]
Item #: B304 -

Product Description of Amine-Reactive BiotinsThe avidin-biotin system has many applications in immunology and histochemistry. The interaction between avidin and biotin is remarkably strong with a dissociation constant on the order of 10-15 M. Biotin is usually added to primary or secondary antibodies such as anti-IgG and anti-IgM. After preparing the antigen-antibody complex with the biotin-labeled antibody, colorimetric, or fluorometric detection of the antigen is performed using enzyme or...

Biotin-PE-maleimide Biotin-PE-maleimide, N-Biotinyl-N E[2-(N-Maleimido)ethyl]piperazone, hydrochloride
Item #: B592 -

Product Description The avidin-biotin system has many applications in immunology and histochemistry. The interaction between avidin and biotin is remarkably strong with a dissociation constant on the order of the 10-15 M. Biotin is usually added to primary or secondary antibodies such as anti-IgG and anti-IgM. After preparing the antigen-antibody complex with the biotin-labeled antibody, colorimetric or fluorometric detection of the antigen is performed using enzyme or fluorophore-labeled...

Biotin-PEAC5-maleimide Biotin-PEAC5-maleimide, N-6-(Biotinylamino)hexanoyl-N’-[2-(N-maleimido)ethyl]piperazone, hydrochloride
Item #: B299 -

Product Description of Amine-Reactive BiotinsThe avidin-biotin system has many applications in immunology and histochemistry. The interaction between avidin and biotin is remarkably strong with a dissociation constant on the order of the 10-15 M. Biotin is usually added to primary or secondary antibodies such as anti-IgG and anti-IgM. After preparing the antigen-antibody complex with the biotin-labeled antibody, colorimetric or fluorometric detection of the antigen is performed using enzyme or...

CLAMP method – A New Highly Sensitive Detection Method for Cell Surface Antigens
Item #: zz99 -

Video Product Description Cell surface antigens that are specifically expressed on cancer and immune cells have been actively studied for early detection and treatment of cancer. However, many cell surface proteins have low expression levels, and most of them are difficult to detect by conventional techniques. A method using a fluorescence-labeled antibody is widely used for a specific detection of cell surface proteins (fluorescent immunostaining method). However, this method cannot be...

DTPA anhydride DTPA anhydride, Diethylenetriamine-N,N,N’,N”,N”-pentaacetic dianhydride [CAS: 23911-26-4]
Item #: D033 -

Product Description of Amine-Reactive Biotins DTPA anhydride is utilized to add chelate functions to molecules or surfaces with amine groups. After adding chelate functions, metal ions such as radioisotopes can be introduced to the molecule. According to Dr. Hnatowich, IgG may be labeled with radioactive indium using DTPA-anhydride. DTPA is first linked to IgG through a covalent bond, then In(III) is tightly chelated to DTPA. Such radioactive antibodies are known to bind with antigen in vitro...

EMCS EMCS, N-(6-Maleimidocaproyloxy)succinimide [CAS: 55750-63-5]
Item #: E018 -

Cross-linking Reaction Product Description Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature because it is necessary to maintain enzyme activity and antibody titers in the cross-linking...

FeBABE FeBABE, 1-(p-Bromoacetamidobenzyl) ethylenediamine N,N,N’,N’-tetraacetic acid, acid iron(III)
Item #: F279 -

Product Description of BABEs Bromoacetamidobenzyl-EDTA (BABE) is a chelate labeling reagent that conjugates with sulfhydryl groups. The iron chelate of BABE (FeBABE) is a unique tool for determining the three-dimensional structure of proteins and the binding structures of protein-protein or protein-DNA complexes. BABE adds EDTA moieties to proteins through their sulfhydryl groups. Once attached to a protein, FeBABE cuts a nearby peptide or DNA chain. The cleavage site is within 12 angstroms of...

Fluorescein Labeling Kit-NH2
Item #: LK01 -

Product Description Fluorescein Labeling Kit-NH2 is mainly used for the preparation of fluorescein-labeled proteins such as IgG for immunostaining and cellular proteins for tracing. Amine-reactive fluorescein, a component of this kit, has succinimidyl groups (NHS) that react with the amino groups on proteins or other molecules (Fig. 1). This kit contains all the reagents necessary for labeling, including storage buffer. Each vial of fluorescein can label up to 200 μg of IgG, conjugating about...

GMBS GMBS, N-(4-Maleimidobutyryloxy)succinimide [CAS: 80307-12-6]
Item #: G005 -

Cross-linking Reaction Product Description Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature because it is necessary to maintain enzyme activity and antibody titers in the cross-linking...

HiLyte Fluor 555 Labeling Kit-NH2
Item #: LK14 -

Product Description HiLyte Fluor* 555 Labeling Kit-NH2 is used mainly for the preparation of red fluorescence-labeled proteins, such as IgG, for immunostaining, and cellular proteins for tracing. NH2-reactive HiLyteFluor 555, a component of this kit, has a succinimidyl group (NHS) that reacts with amino groups on proteins or other molecules (Fig. 1). This kit contains all the reagents necessary for labeling. Each tube of HiLyte Fluor 555 can label up to 200 μg of IgG, conjugating about 4 to 6...

HiLyte Fluor 647 Labeling Kit-NH2
Item #: LK15 -

Product Description HiLyte Fluor* 647 Labeling Kit-NH2 is mainly used for the preparation of red fluorescence-labeled proteins, such as IgG, for immunostaining, and cellular proteins for tracing. NH2-reactive HiLyteFluor 647, a component of this kit, has a succinimidyl group (NHS) that reacts with amino groups on proteins or other molecules (Fig. 1). This kit contains all the reagents necessary for labeling. Each tube of HiLyte Fluor 647 can label up to 200 μg of IgG, conjugating about 4 to 6...

IC3-OSu special packaging IC3-OSu special packaging, N-Ethyl-N’-[5-(N’-succinimidyloxycarbonyl)pentyl]indocarobcyanine chloride
Item #: I271 -

Product Description IC3 and IC5 are indocyanine dyes that can react with aminegroups on proteins, peptides, and amine-modified oligonucleotides. Because neither IC3 nor IC5 has a sulfonate group in its structure, water solubility of the molecules is very low. An organic solvent is required to dissolve the compound. Over-labeling of proteins with these compounds may precipitate the labeled protein from aqueous solutions as a result of the poor water solubility. However, because no negative...

IC5-OSu special packaging IC5-OSu special packaging, N-Ethyl-N’-[5-(N’-succinimidyloxycarbonyl)pentyl]-3,3,3”,3”-tetramethyl-2,2’-indocarobcyanine chloride
Item #: I272 -

Product Description IC3 and IC5 are indocyanine dyes which can react with aminegroups on proteins, peptides, and amine-modified oligonucleotides. Since both IC3 and IC5 do not have a sulfonate group in the structure, water-solubility of the molecules is very low. An organic solvent is required to dissolve the compound. Over labeling of proteins with these compounds may precipitate the labeled protein from aqueous solutions due to the poor water-solubility. However, since no negative charge is...

ICG Labeling Kit - NH2 ICG Labeling Kit - NH2
Item #: LK31 -

Product Description ICG Labeling Kit - NH2 is used primarily for the preparation of ICG (Iindocyanin green)-labeled antibody for near-infrared fluorescence imaging. ICG offers two remarkable properties: 1) ICG has a strong near-infrared fluorescence even after a few days under physiological conditions. The excitation and emission wavelength of the ICG-labeled proteins are 774 nm and 805 nm, respectively. 2) ICG has been used in clinical fields such as a hepatic deficiency testing. Therefore,...

ICG-Sulfo-OSu ICG-Sulfo-OSu, 2-[7-[1,3-Dihydro-1,1-dimethyl-3-(4-sulfobutyl)-2H-benzo[e]indol-2-ylidene]-1,3,5-heptatrienyl]- 1,1-dimethyl-3-[5-(3-sulfosuccinimidyl)oxycarbonylpentyl]-1H-benzo[e]indolium, inner salt, sodium salt
Item #: I254 -

Product Description ICG is one of the dyes that is used for determining cardiac output, hepatic function, and liver blood flow, as well as for ophthalmic angiography. It has a long excitation wavelength and emission wavelength of about 780 nm and 800 nm, respectively. Because of its long wavelength near the infrared region and low cytotoxicity, ICG is used to label antibodies for in vivo assay. However, fluorescent intensity after conjugation with protein is quite low because of the formation...

Isothiocyanobenzyl-EDTA Isothiocyanobenzyl-EDTA, 1-(4-Isothiocyanatobenzyl)ethylenediamine-N,N,N’,N’- tetraacetic acid [CAS: 105394-74-9]
Item #: M030 -

Product Description of Amine-Reactive Biotins Isothiocyanobenzyl-EDTA adds EDTA moieties to proteins through their amine groups. The EDTA-tagged protein can then be labeled with heavy metal ions and radioactive metals, such as 111In and 99Tc, and used for immunoimaging. Chemical Structure <Caution> When it is difficult to take out all the powder from the container, please add the solvent into a container and dissolve it before its use. ...

Isothiocyanobenzyl-NTA 16-Amino-1-hexadecanethiol, hydrochloride, 16-Amino-1-hexadecanethiol, hydrochloride
Item #: I279 -

Product Description Isothiocyanobenzyl-NTA is utilized to modify the surfaces on which amine groups are attached. Through the NTA moiety attached on the surface, genetically expressed protein, bearing a hexahistidine extension at its terminus, can be immobilized via Ni (II) (His-Tag method). Using this technique, Dr. Noji and co-workers were able to directly abserve the rotation of F1-ATPase with a fluorescence microscope. Chemical Structure ...

Maleimido-C3-NTA Maleimido-C3-NTA, N-(5-(3-Maleimidopropylamido)-1-carboxy-pentyl)iminodiacetic acid, disodium salt, monohydrate
Item #: M035 -

Product Description Maleimido-C3-NTA is utilized to modify the surfaces on which thiol groups are attached. Through the NTA moiety attached to the surface, genetically expressed protein, bearing a hexahistidine extension at its terminus, can be immobilized via Ni (II) (His-Tag method). Using this technique, Dr. Noji and co-workers were able to directly observe the rotation of F1-ATPase with a fluorescence microscope. Chemical Structure <Caution> When it is difficult to take out all the...

MDB MDB, 1,2-Diamino-4,5-methylenedioxybenzene, dihydrochloride [CAS: 38608-07-0]
Item #: M021 -

Derivatization Reaction Product Description Phenylenediamine (OPD) reacts with α-keto acids to form highly fluorescent quinoxaline derivatives. MDB is an o-phenylenediamine analogue. It is the best labeling reagent for α-keto acids because of its reactivity and sensitivity. In HPLC analysis, more than ten different α-keto acids, such as α-keto glutaric acid, pyruvic acid, and phydroxyphenyl-pyruvic acid, can be separately detected at a time, even at concentrations of only a few femtomoles per...

NBD-F NBD-F, 4-Fluoro-7-nitrobenzofurazan [CAS: 29270-56-2]
Item #: N020 -

Derivatization Reaction Product Description NBD-F is highly reactive and can label primary and secondary amines under mild conditions (1 min reaction at 60ºC in a weak basic solution). NBD-F is a pre-labeling compound for HPLC analysis of small molecules. NBD-labeled compounds are orange with a maximum wavelength at 470 nm.The excitation and emission of the derivatized compound are 470 nm and 530 nm, respectively. ...

Peroxidase Labeling Kit-NH2
Item #: LK11 -

LK11-10 LK51-10 Product Description Peroxidase Labeling Kit-NH2 is used mainly for the preparation of peroxidase-labeled IgG for enzyme immunoassay (EIA) and for the preparation of peroxidase-labeled antigen for competitive EIA. NH2-reactive peroxidase, a component of this kit, has succinimidyl groups (NHS) and reacts with proteins or other molecules that have an amino group in their structures (Fig. 1). This kit contains all the reagents necessary for the labeling process, including storage...

Peroxidase Labeling Kit-SH
Item #: LK09 -

Product Description Peroxidase Labeling Kit-SH is used mainly for the preparation of peroxidase-labeled IgG for enzyme immunoassay (EIA) and for the preparation of peroxidase-labeled antigen for competitive EIA. SH-reactive peroxidase, a component of this kit, can react with the thiol groups of proteins or other molecules (Fig. 1). This kit contains all the necessary reagents for the labeling process, including reducing agent and storage buffer. SH-reactive peroxidase forms a covalent link...

R-Phycoerythrin Labeling Kit-NH2
Item #: LK23 -

Product Description Phycobiliproteins are fluorescent proteins derived from cyanobacteria and eukaryotic algae. Their fluorescence is much higher than chemical fluorescent probes such as fluorescein and rhodamine. R-Phycoerythrin (R-PE) is one of the phycobiliproteins and has a red fluorescence at around 578 nm, and it can be excited at 488nm (Fig. 1). Because of this high fluorescence, phycobiliprotein labeled antibodies, and other molecules can provide greater sensitivity in flow cytometry...

R-Phycoerythrin Labeling Kit-SH
Item #: LK26 -

Product Description Phycobiliproteins are fluorescent proteins derived from cyanobacteria and eukaryotic algae. Their fluorescence is much higher than chemical fluorescent probes such as fluorescein and rhodamine. R-phycoerythrin (R-PE) is one of the phycobiliproteins and has an orange fluorescence at around 578 nm, and it can be excited at 488 nm (Fig. 1). Because of this high fluorescence, phycobiliprotein-labeled antibodies or other molecules can give greater sensitivity in flow cytometry...

SBD-F SBD-F, 4-Fluoro-7-sulfobenzofurazan, ammonium salt [CAS: 84806-27-9]
Item #: S013 -

Derivatization Reaction Product Description SBD-F is a water-soluble reagent that reacts with sulfhydryl groups to produce highly fluorescent compounds. The detection limit of thiol compounds such as glutathione, cysteine, N-acetylcysteine, CoA and BSA by HPLC analysis is in the range of 100-500 pmol per injection.The excitation and emission of the derivatized compound are 385 nm and 515 nm, respectively. ...

SPDP SPDP, N-Succinimidyl 3-(2-pyridyldithio)propionate [CAS: 68181-17-9]
Item #: S291 -

Product Description SPDP, a hetero-bifunctional reagent, is useful for bridging two different proteins, such as enzymes and antibodies. SPDP first reacts with a protein molecule through its amino groups. SPDP introduces a pyridyl disulfide moiety to the protein, which is then reduced by DTT to form thiol groups. These thiol groups then form a disulfide bond with another protein molecule thus yielding a heterodimer protein. Structural Formula ...

Sulfo-EMCS Sulfo-EMCS, N-(6-Maleimidocaproyloxy)sulfosuccinimide, sodium salt
Item #: S024 -

Cross-linking Reaction Product Description Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature because it is necessary to maintain enzyme activity and antibody titers in the cross-linking...

Sulfo-GMBS Sulfo-GMBS, N-(4-Maleimidobutyryloxy)sulfosuccinimide, sodium salt
Item #: S025 -

Cross-linking Reaction Product Description Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature because it is necessary to maintain enzyme activity and antibody titers in the cross-linking...