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Item # Unit Size
10 mg

For Research Use Only Products

Chemical Name: N-(5-(3-Maleimidopropylamido)-1-carboxy-pentyl)iminodiacetic acid, disodium salt, monohydrate

Appearance: White pale yellowish-white powder
Purity: ≥97.0% (HPLC)
MW: 489.38 C18H23N3Na2O9 · H2O
Solubility in water: Soluble, 10mg/ml

Storage Condition: 0-5ºC
Shipping Condition: ambient temperature

Product Description
Maleimido-C3-NTA is utilized to modify the surfaces on which thiol groups are attached. Through the NTA moiety attached to the surface, genetically expressed protein, bearing a hexahistidine extension at its terminus, can be immobilized via Ni (II) (His-Tag method). Using this technique, Dr. Noji and co-workers were able to directly observe the rotation of F1-ATPase with a fluorescence microscope.

Chemical Structure

When it is difficult to take out all the powder from the container,
please add the solvent into a container and dissolve it before its use.
1) E. Hochuli, H. Doeli and A. Schacher, New Metal Chelate Adsorbent Selective for Proteins and Peptides Containing Neighbouring Histidine Residues, J. Chromatogr., 1987, 411, 177.
2) E. Hochuli, Large-Scale Chromatography of Recombinant Proteins, J. Chromatogr., 1988, 444, 293.
3) Y. C. Sasaki, Y. Suzuki and T. Ishibashi, Fluorescent X-ray Interference from a Protein Monolayer, Science, 1994, 263, 62.
4) G. B. Sigal, C. Bamdad, A. Barberis, J. Strominger and G. M. Whitesides, A Self-Assembled Monolayer for The Binding and Study of Histidine-Tagged Proteins by Surface Plasmon Resonance, Anal. Chem., 1996, 68, 490.
5) E. L. Schmid, T. A. Keller, Z. Dienes and H. Vogel, Reversible Oriented Surface Immobilization of Functional Proteins on Oxide Surface, Anal. Chem., 1997, 69, 1979.