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Item # Unit Size
50 mg
100 mg

For Research Use Only Products

Chemical Name: N-(6-Maleimidocaproyloxy)succinimide
CAS: 55750-63-5

Appearance: white or slightly yellow powder
Purity: ≥90.0% (HPLC)
MW: 308.29, C14H16N2O6

Storage Condition: 0-5ºC
Shipping Condition: ambient temperature

Cross-linking Reaction

Product Description
Hetero-bifunctional cross-linking reagents have activated esters and maleimide reactive groups. These functional groups react with amines and sulfhydryl groups of proteins, respectively. Enzyme-labeled haptens are prepared using hetero-bifunctional cross-linking reagents such as EMCS or GMBS. The cross-linking reaction requires neutral pH and mild temperature because it is necessary to maintain enzyme activity and antibody titers in the cross-linking reaction. Hetero-bifunctional cross-linking reagents with 3, 5, 7, or 10 linear carbon chains are available. These linear aliphatic chains act as spacers between the two reactive sites and their water-soluble reagents. They are more stable than the aromatic cross-linking reagents such as succinimidyl-4-N-maleimidobenzoate in a wider pH range.

Conjugation of Macromolecules with Hetero-Bifunctional Cross-Linking Reagent

Hetero-bifunctional Reagents
Product Name Code Length (Å) Solvent
EMCS E018 9.4 DMSO
GMBS G005 6.9 Chloroform, DMF

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4) E. Ishikawa, M. Imagawa, S. Hashida, S. Yoshitake, Y. Hamaguchi and T. Ueno, Enzyme-Labelling of Antibodies and Their Fragments for Enzyme Immunoassay and Immunohistochemical Staining, J. Immunol., 1983, 4, 209.
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7) S. Inoue, M. Imagawa, S. Hashida, K. Ruan and E. Ishikawa, A Small Scale Preparation of Affinity-Purified Rabbit Fab EHorseradish Peroxidase Conjugate for Enzyme Immunoassay, Anal. lett., 1984, 17, 229.
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9) T. Kohno and E. Ishikawa, Novel Enzyme Immunoassay(Immune Complex Transfer Enzyme Immunoassay) for Anti-insulin IgG in Guinea Pig Serum, Anal. lett., 1988, 21, 1019.