-Bacstain-DAPI Solution

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-Bacstain-DAPI Solution

Item #	Unit Size
BS04-10	100 assays

For Research Use Only Products

Fluorescence detection

Ready-to-use solution

Chemical Name: 4',6-Diamidino-2-phenylindole, dihydrochloride, solution
CAS: 28718-90-3 (DAPI)

Contents of the kit:
DAPI Solution: 25 µl x 4 vials

Storage Condition : 0-5ºC, protect from light
Shipping Condition : ambient temperature

Required Equipment and Materials
10 μl, 1000 μl pipettes, incubator, Microscope (blue excitation filter and red emission filter) or flow cytometer (360 nm violet laser)

Chemical Structure

Staining procedure

1. Allow DAPI solution^a) to stand at room temperature for 30 minutes. Solution should be protected from light.
2. Resuspend the organisms with PBS(-) or saline and adjust the number of cells to 10⁶cells/mL(flow cytometry) or 10⁸-10⁹cells/mL(microscopy).
3. Add 1 μL of DAPI solution into the microbial cell suspension and vortex gently to mix. Formaldehyde-fixation can be recommended if necessary.
4. Incubate the microbial cells at room temperature for 5 minutes.
5. Analyze the stained-cells by a flow cytometer or a microscope. The maximum wavelengths of the dye are 360 nm for excitation and 460 nm for emission.

a) Since DAPI may be carcinogenic, be careful when handling and disposing.

Staining Data

Fig. 2 L. casei stained with CTC and DAPI (left). B. cereus stained with CFDA and DAPI (right).