Chemical Name: Fluorescein diacetate
Appearance: white crystalline powder
MW: 416.38, C24H16O7
Storage Condition : -20ºC, protect from light
Shipping Condition : ambient temperature
FDA is cell-membrane permeable and accumulates inside of viable cells as fluorescein (Fig. 1). Since fluorescein is less hydrophilic than BCECF or Calcein, the leakage of fluorescein from cells is rather high. FDA is also utilized for flow cytometry. The excitation and emission wavelengths of fluorescein are 488 nm and 530 nm, respectively. FDA-stained cells are shown in Fig. 2.
Fig. 1 Cell staining mechanism