Chemical Name: 1-Hydroxy-2-oxo-3,3-bis(2-aminoethyl)-1-triazene
Appearance: White powder
Purity: ≥90% (HPLC)
MW: 163.18, C4H13N5O2
Storage Condition : -20ºC, protect from light and moisture
Shipping Condition: with blue ice or dry ice
Reaction of NO release
Product Description of NOC Compounds
are stable NO-amine complexes that spontaneously release NO, without
cofactors, under physiological conditions. The rate of NO release
depends on the chemical structure of NOC. The mechanism of spontaneous
NO generation by NOCs is very simple compared to other classical NO
donors, such as nitroglycerin and nitropurusside, and the by-products do
not interfere with cell activities. A single NOC molecule releases two
NO molecules (as indicated in the reaction scheme); the release rate of
the second NO molecule is very slow. NOCs can be used to add controlled
amounts of pure NO to experimental systems at controlled rates with
minimal side effects. The amount of NO released can be easily
manipulated by altering the concentration and selection of NOC reagents.
Dojindo offers four different NOCs (NOC 5, 7, 12, and 18) with
different halflifes. Stock solutions of NOC prepared in alkaline
solutions, such as aqueous NaOH, are relatively stable. However,the NOC
stock solution should be used within one day because it degrades about
5% per day, even at -20ºC. The release of NO begins immediately after
adding the stock solution to a sample solution.
Nitric Oxide Release
Prepare 10 mM NOC stock solution using 0.1 M NaOH. Since the NOC stock
solution is not stable, keep it on an ice bath and use it in one day.
Add an appropriate volume of the NOC stock solution to the sample
solution in which NO is to be released. To maintain the pH of the sample
solution, the volume of the NOC stock solution should not exceed 1/50
of the sample volume. The sample solution should have sufficient
buffering action. NO will be released immediately after the addition of
the NOC stock solution.Table 1 pH Dependency of NO Release at 37oC
K. Hayashi, et al., Action of Nitric Oxide as a Antioxidant Against
Oxidation of Soybean Phosphatidylcholine Liposomal Membrane. FEBS Lett.
1995;370:37-40. (NOC 12)2.
S. Shibuta, et al., Intracerebroventricular Administration of a Nitric
Oxide-releasing Compound, NOC-18, Produces Thermal Hyperalgesia in Rats.
Neurosci Lett. 1995;187:103-106. (NOC 18)3.
S. Shibuta, et al., A new nitric oxide donor, NOC-18, exhibits a
nociceptive effect in the rat formalin model. J Neurol Sci. 1996;141:1-5. (NOC 18)
4. N. Yamanaka, et al., Nitric Oxide Released from Zwitterionic Polyamine/NO Adducts Inhibits Cu2+-induced Low Density Lipoprotein Oxidation. FEBS Lett. 1996;398:53-56. (NOC 5, NOC 7)5. D. Berendji, et al., Nitric Oxide Mediates Intracytoplasmic and Intranuclear Zinc Release. FEBS Lett. 1997;405:37-41.6. T. Ohnishi, et al., The Effect of Cu2+ on Rat Pulmonary Arterial Rings. Eur J Pharmacol. 1997;319:49-55. (NOC 7)7. Y. Adachi, et al., Renal Effect of a Nitric Oxide Donor, NOC 7, in Anethetized Rabbits. Eur J Pharmacol. 1997;324:223-226. (NOC 7)8. Y. Minamiyama, et al., Effect of Thiol Status on Nitric Oxide Metabolism in the Circulation. Arch Biochem Biophys. 1997;341:186-192. (NOC 7)
How do I prepare a stock solution?
Prepare 10-50 mM NOC solution with 0.1 M NaOH solution. Then add enough NOC solution to the cell culture to obtain a suitable concentration of NOC in cell culture. If the pH of the culture solution changes, use higher concentration of NOC.
What is the solubility of the NOC compounds?
NOC 5: 40 mg per 100 ml 0.1 M NaOH (2.2 M NOC 5)
NOC 7: 70 mg per 100 ml 0.1 M NaOH (4.3 M NOC 7)
NOC 12: 27 mg per 100 ml 0.1 M NaOH (1.5 M NOC 12)
NOC 18: 20 mg per 100 ml 0.1 M NaOH (1.2 M NOC 18)
Is the stock solution stable?
The stock solution will lose 5% of its NOC activity per day, even when stored at -20oC. Please prepare fresh solution prior to use and keep the solution on an ice bath during the experiment. VI-1. Nitric Oxide Research: NO Donors
How is the half-life of NOC determined?
Prepare 20 mM NOC stock solution with 0.1 M NaOH. Warm PBS at 37oC. Add 100 ml NOC solution to 1.9 ml PBS. Using a UV spectrophotometer, immediately start measuring its absorbance at the maximum wavelength of the NOC. Continue measuring until no further spectra changes are observed.
Can I use NOC for in vivo experiments?
Yes. Please review the papers by Shibata and colleagues (1995, 1996).
Is the amount of NO released in vitro the same as in vivo?
The amount of NO released in the solution should be the same if the pH and temperature are the same. However, the activity of NO may be different in vivo because of other reactive components such as thiol compounds and heme.