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Item # Unit Size
100 mg
1 g

For Research Use Only Products

Application: Dehydrogenase detection, staining

Chemical Description: 3,3'-[3,3'-Dimethoxy-(1,1'-biphenyl)-4,4'-diyl]-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride
CAS: 298-83-9

Appearance: yellow crystalline powder
Purity: ≥98.0%
Molar absorptivity: ≥63,000 (around 257 nm)
MW: 817.64, C40H30Cl2N10O6

Storage Condition: ambient temperature, protect from light
Shipping Condition: ambient temperature

Reaction Scheme

Product Description
Nitro-TB is dissolved by hot water or hot methanol. Other organic solvents such as acetone and ether cannot solubilize Nitro-TB. Nitro-TB is easily reduced by dehydrogenase to purple formazan dye aggregates. It is used for dehydrogenase detection on agarose gels.
1) K. Tsou, et al., Synthesis of Some p-Nitrophenyl Substituted Tetrazolium Salts Electron Acceptors for the Demonstration of Dehydrogenases. J Am Chem Soc. 1956;78:6139-6144.
2) J. R. Baker, et al., Mechanism of Fructosamine Assay: Evidence against Role of Superoxide as Intermediate in Nitroblue Tetrazolium Reduction. Clin Chem. 1993;39:2460-2465.