10 Redox Dyes

TB

TB

Reductive Chromogenic Dye

  • Product code
    T012  TB
  • CAS No.
    1871-22-3
  • Chemical name
    3,3'-[3,3'-Dimethoxy-(1,1'-biphenyl)-4,4'-diyl]bis(2,5-diphenyl-2H-tetrazolium chloride)
  • MW
    C40H32Cl2N8O2=727.64
Unit size Price Item Code
1 g T012-10

Reaction Scheme

Product Description

TB is slightly soluble in water, but does not dissolve easily in acetone, ethyl acetate, and ether.

Other organic solvents such as methanol, ethanol, and tetrachloromethane can solubilize TB pretty well.

Since TB is easily reduced with dehydrogenase to generate reddish-purple formazan dye aggregates, it is used for dehydrogenase detection in tissue samples.

References

Open References

1. K. Tsou, et al., Synthesis of Some p-Nitrophenyl Substituted Tetrazolium Salts Electron Acceptors for the Demonstration of Dehydrogenases. J Am Chem Soc. 1956;78:6139-6144.
2. S. S. Karmarkar, et al., Preparation of Nitrotetrazolium Salts Containing Benzothiazole. J Org Chem. 1960;25:575-578.
3. J. E. Sinsheimer, et al., Reactivity of Blue Tetrazolium with Nonketol Compounds. Anal Chem. 1965;37:566-569.

Handling and storage condition

Specification
Appearance: Pale yellow to pale yellowish orange powder
Solubility in water: To pass test (clear, yellow)
Solubility in Methyl alcohol: To pass test (clear, yellow)
m.p.: ≧ 248℃ (dec.)
Molar absorptivity: ≧ 52,500 (around 254 nm)
Sulfated ash: ≦0.20%
Sensitivity: To pass test
IR spectrum: Authentic
Handling and storage condition
Ambient temperature
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Product Classification

Product Classification

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