Application: Dehydrogenase detection, staining
Chemical Description: 3,3'-[3,3'-Dimethoxy-(1,1'-biphenyl)-4,4'-diyl]-bis(2,5-diphenyl-2H-tetrazolium chloride)
Appearance: pale yellow or pale orange-yellow powder
Molar absorptivity: >52,500 at 254 nm
MW: 727.64, C40H32Cl2N8O2
Storage Condition: ambient temperature, protect from light
Shipping Condition: ambient temperature
TB is slightly soluble in water, but does not dissolve easily in acetone, ethyl acetate, and ether. Other organic solvents such as methanol, ethanol, and tetrachloromethane can solubilize TB pretty well. Since TB is easily reduced with dehydrogenase to generate reddish-purple formazan dye aggregates, it is used for dehydrogenase detection in tissue samples.
1) K. Tsou, et al., Synthesis of Some p-Nitrophenyl Substituted Tetrazolium Salts Electron Acceptors for the Demonstration of Dehydrogenases. J Am Chem Soc. 1956;78:6139-6144.
2) S. S. Karmarkar, et al., Preparation of Nitrotetrazolium Salts Containing Benzothiazole. J Org Chem. 1960;25:575-578.
3) J. E. Sinsheimer, et al., Reactivity of Blue Tetrazolium with Nonketol Compounds. Anal Chem. 1965;37:566-569.