Chemical Name: Coelenterazine, β-cyclodextrin complex
Appearance: pale yellowish-brown powder
Solubility (in 0.1 mol/l phosphate buffer): To pass test (clear, slightly yellow)
Storage Condition: -20ºC, protect from light and moisture
Shipping Condition: with blue ice
Aequorin is a luminescent protein that contains coelenterazine as a luminescent compound. Since aequorin emits luminescence by calcium conjugation, it is used for intracellular calcium ion detection. However, coelenterazine has poor water solubility under physiological conditions, and it is adsorbed to cell membranes. Dojindo’s Coelenterazine-WS is a β-cyclodextrin complex of coelenterazine with drastically improved water solubility at neutral pH.
1) K. Teranishi, et al., Solubilizing Coelenterazine in Water with Hydroxypropyl-β-cyclodextrin. Biosci Biotech Biochem. 1997;61:1219-1220.