Chemical Name: 1-[2-Amino-5-(3-dimethylamino-6-dimethylammonio-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, chlorid
Appearance: Dark purple solid
Purity: ≥60.0 %(HPLC)
MW: 791.24, C40H43ClN4O11
Storage Condition: -20ºC, protect from light
Shipping Condition: ambient temperature
Rhod 2 has the longest fluorescent wavelength signal of all the calcium indicators. It has a rhodamine-like fluorophore whose excitation and emission maxima are 557 nm and 581 nm, respectively. This makes it a convenient excitation source for argon and krypton lasers. Although it was thought that the fluorescent signal of Rhod 2 only increases several times with the calcium complex, Dojindo’s Rhod 2 increases its signal with calcium 80-100 times because of its high purity. Its signal intensity is the strongest of all the calcium probes. Rhod 2 is thus highly recommended as a probe for intracellular calcium monitoring using laser microscopes. Rhod 2 is reported to have a better loading profile at the point of localization, especially in neural slice cultures. The dissociation constant of Rhod 2 with calcium (Kd=1.0 mM) is the highest of all the fluorescent calcium probes, providing a wider range for monitoring calcium concentration. Rhod 2-AM is an acetoxymethyl ester derivative of Rhod 2 that can be easily loaded into cells using the AM method.
1. M. Burnier, et al., Confocal Microscopy to Analyze Cytosolic and Nuclear Calcium in Cultured Vascular Cells. Am J Physiol. 1994;266:C1118-C1127.
2. J. Vergara, et al., Imaging of Calcium Transients in Skeletal Muscle Fibers. Biophys J. 1991;59:12-24.
3. T. Meyer, et al., Kinetics of Calcium Channel Opening by Inositol 1, 4, 5-Trisphosphate. Biochemistry. 1990;29:32-37.
4. A. Minta, et al., Fluorescent Indicators for Cytosolic Calcium Based on Rhodamine and Fluorescein Chromophores. J Biol Chem. 1989;264:8171-8178.
5. D. R. Trollinger, et al., Selective Loading of Rhod 2 into Mitochondria Shows Mitochondrial Ca2+ Transients during the Contractile Cycle in Adult Rabbit Cardiac Myocytes. Biochem Biophys Res Commun. 1997;236:738-742.