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Item # Unit Size
25 mg

For Research Use Only Products

Chemical Name: Bis(1,3-dibutylbarbituric acid)trimethine oxonol, sodium salt

Appearance: Reddish-orange to red powder
Purity: ≥98.0% (HPLC)
MW: 538.61, C27H39N4NaO6

Storage Condition: 0-5ºC
Shipping Condition: ambient temperature

Product Description
DiBAC4(3) is a Bis-oxonol-type, membrane-potential, sensitive dye. According to membrane depolarization detection, when DiBAC distribution in cytosol is increased, the fluorescence intensitiy is increased. Since an argon laser (488 nm) can be used for DiBAC4(3) excitation, it is applicable for flow cytometry and confocal microscopy.

Chemical Structure

1) D. E. Epps, et al., Characterization of the Steady-state and Dynamic Fluorescence Properties of the Potential-sensitive Dye Bis-(1,3-dibutylbarbituric acid)trimethine oxonol(Dibac4(3)) in Model Systems and Cells. Chem Phys Lipids. 1994;69:137-150.
2) T. Brauner, et al., Comparative Measurements of Membrane Potentials with Microelectrodes and Voltage-sensitibe Dyes. Biochim Biophys Acta. 1984;771:208-216.
3) T. T. Rohn, et al., Xanthine Affects [Ca2+]i and Contractile Responses of Ventricular Cardiocytes to Electrical Stimulation. Am J Physiol. 1997;273:C909-C917.
4) D. J. Mason, et al., Rapid Estimation of Bacterial Antibiotic Susceptibility with Flow Cytometry. J Microsc. 1994;176:8-16.
5) U. Langheinrich, et al., Hyperpolarization of Isolated Capillaries from Guinea-pig Heart Induced by K+ Channel Openers and Glucose Deprivation. J Physiol. 1997;502:397-408.
6) K. S. Schroeder, et al., FLIPR: A New Instrument for Accurate, High Throughput Optical Screening. J Biomol Screen. 1996;1:75-80.