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Item # Unit Size
100 mg

For Research Use Only Products

Application:Zn2+ detection, colorimetric

Chemical Name: 2-(5-Bromo-2-pyridiylazo)-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol, disodium salt, dihydrate
CAS: 81608-06-2 (free acid)

Appearance: Orange or reddish-brown crystalline powder
Absorbance (0.01 mmol/l): ≥0.420 (around 449 nm)
Absorbance (0.01 mmol/l): ≤0.040 (at 552 nm)
MW: 537.34, C17H19BrN4Na2O4S,2H2O

Storage Condition: ambient temperature, protect from metal and moisture
Shipping Condition: ambient temperature

Product Description
5-Br-PAPS, a water-soluble derivative of 5-Br-PADAP, is a highly sensitive colorimetric reagent for zinc detection. This reagent forms a red chelate with zinc at pH 7.5-9.5 (λmax=552 nm, λ=13.3 x 104). 5-Br- PAPS is also utilized for the determination of Cu(II), Fe(II), Ni(II), Co(II), Pt(II), Ru(II), and Rh(II). 5-Br-PAPS is utilized to determine serum levels of Zn, Cu, Fe, Ni, and Co. For serum Zn levels, Fe ions can be masked with citric acid or metaphosphoric acid, and fluoride and Cu ions can be masked with 2-mercaptobenzothiazole or dithiocarboxysarcosine. 5-Br-PAPS-Ti complex can be used for hydrogen peroxide detection.

Chemical Structure
1) T. Makino, et al., A Highly Sensitive Colorimetric Determination of Serum Zinc Using Water-soluble Pyridylazo Dye. Clin Chim Acta. 1982;120:127-135.
2) D. Horiguchi, et al., Water Soluble Pyridylazoaminophenols and Pyridylazoaminobenzoic Acids as Highly Sensitive Photometric Reagents for Zinc, Uranium, Cobalt and Nickel. Anal Sci. 1985;1:461-465.
3) C. Matsubara, et al., Spectrophotometric Determination of Hydrogen Peroxide with Titanium 2-((5-bromopyridyl)azo)-5-(N-proryl-N-sulfopropylamino) phenol Reagent and Its Application to the Determination of Serum Glucose Using Glucose Oxidase. Anal Chem. 1985;57:1107-1109.
4) Y. Shijo, et al., Spectrophotometric and Analogue Derivative Spectrophotometric Determination of Chromium(III) with 2-(5-Bromo-2-pyridylazo)-5-(NOpropyl-N-sulfopropylamino)phenol. Bull Chem Soc Jpn. 1986;59:1455-1458.
5) Y. Shijo, et al., Spectrophotometric and Analogue Derivative Spectrophotometric Determination of Rhodium(III) with 2-(5-Bromo-2-pyridylazo-5-propyl-N-sulphopropylamino)phenol. Analyst. 1988;113:519-521.
6) Y. Shijo, et al., High Performance Liquid Chromatographic Separation of Iron, Bismuth, Indium and Thallium by Pre-column Chelation with 2-(5-Bromo-2-pyridylazo)-5-(N-propyl-N-sulphopropylamino)phenol. Analyst. 1988;113:1201-1203.
7) C. Ohtsuka, et al., Reversed-phase Ion-pair Partition Liquid Chromatoguraphy of Chelates with 2-(3,5-Dibromo-2-pyridylazo)-5-[N-ethyl-N-(3-sulphopropyl) amino]phenol and Analogues. Anal Chim Acta. 1989;223:339-347.
8) I. Kasahara, et al., Bis[2-(5-Bromo-2-pyridylazo)-5-(N-propyl-N-sulphopropylamino)phenolato]cobaltate(III) as a Counter Ion for the Extraction and Spectrophotometric Determination of Long-chain Quaternary Ammonium Salts and Tertiary Alkylamines in the Presence of Each Other. Anal Chim Acta. 1989;219:239-245.
9) N. Uehara, et al., Simultaneous Determination of Platinum(II), Rhodium(III) and Palladium(II) with 2-(5-Bromo-2-pyridylazo)-5-(N-propyl-N-sulfopropylamino) phenol by High Performance Liquid Chromatography. Anal Sci. 1989;5:111-112.
10) Y. Hayashibe, et al., Direct Determination of Zinc in Human Serum by Flow-injection Spectrophotometric Analysis. Anal Sci. 1994; 10:795-799.
11) N. Uehara, et al., Determination of Cobalt in Natural Water as a 2-(5-Bromo-2-pyridylazo)-5-[N-propyl-N-(3-sulphopropyl)amino]phenol Chelate by On-Line Preconcentration HPLC with Column-Switching Technique. Anal Sci. 1998;14:343-348.
Table 1 Spectral Data of 5-Br-PAPS-Metal Complex