Application: Alkaline earth metals detection, colorimetric
ChemicalName: 2,7-Bis(4-chloro-2-phosphonophenylazo)-1,8-dihydroxy-3,6-naphthalenedisulfonic acid
Appearance: Reddish brown or black purple crystalline powder
Absorbance (0.018 mmol/l): ≥0.530 (around 550 E570 nm)
MW: 757.37, C22H16Cl2N4O14P2S2
Storage Condition: ambient temperature, protect from metal
Shipping Condition: ambient temperature
Chlorophosphonazo-III is a colorimetric reagent for thorium, uranium, lanthanoids, actinoids, and alkaline earth metals. This reagent is readily soluble in water and is violet at pH 4 or lower, blue in NaOH solution, and brilliant green in acids such as H2
, HCl and diluted HNO3
(it decomposes in concentrated HNO3
). Its proton dissociation constants are reported to be -1.1, -1.1, 0.6, 0.8, 1.5, 2.5, 5.47, 7.20, 12.15 and 15.13 (μ=0.1, 20ºC). Chlorophosphonazo-III is used as a selective colorimetric reagent for calcium in a mixed Ca/Mg sample in the pH range of 2-3. It is also a useful indicator for the precipitation titration of SO42-
as a standard solution.
1) L. R. Snyder, et al., Characterization and Routine Determination of Nonbasic Nitrogen Types in Cracked Gas Oils by Linear Elution Adsorption Chromatography. Anal Chem. 1964;36:767-773.
2) J. W. Ferguson, et al., Simultaneous Spectrophotometric Determination of Calcium and Magnesium with Chlorophosphonazo-III. Anal Chem. 1964;36:796-799.
3) D. S. Howell, et al., Ultramicro Spectrophotometric Determination of Calcium in Biologic Fluids. Anal Chem. 1966;38:434-438.
4) T. Yamamoto, Extraction-photometric Determination of Uranium(IV) with Chlorophosphonazo-III. Anal Chim Acta. 1973;65:329-334.
5) M. Zenki, et al., Repetitive Determination of Calcium Ion and Regeneration of a Chromogenic Reagent Using Chlorophosphonazo III and an Ion Exchanger in a Circulatory Flow Injection System. Anal Sci. 2002;18:1137-1140.
Table 1 Metal Detection Conditions Using Chlorophosphonazo-III