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Item # Unit Size
100 mg
1 g

For Research Use Only Products

Application: Fe(II) detection, colorimetric

ChemicalName: 2-Nitroso-5-[N-n-propyl-N-(3-sulfopropyl)amino]phenol
CAS: 80459-15-0

Appearance: Yellow or yellowish brown powder
Purity: ≥97.0% (Absorbance)
Molar absorptivity (Fe2+ complex): ≥44,000 (around 756 nm)
Absorbance (Fe2+ free, Blank): ≤0.050 (around 756 nm)
MW: 302.35 C12H18N2O5S

Storage Condition: 0-5ºC, protect from metal
Shipping Condition: ambient temperature

Product Description
Nitroso-PSAP is a highly sensitive water-soluble colorimetric reagent for Fe(II) detection that forms a green complex with Fe(II) (λmax=756 nm, ε=4.5x104 ). This reagent is suitable for the determination of blood serum levels of Fe, Co, Ni, and Cu. The detection range is from 5 ppb to 5 ppm under the same detection condition. Nitroso-PSAP is useful in flow injection analyses. The workable pH range of the nitroso compounds is 5.6-10.1. The molar absorptivities and maximum wavelengths of the Co, Cu, and Ni complexes are 4.0x104 (490 nm), 2.8x104 (429 nm), and 2.6x104 (394 nm), respectively.

Chemical Structure

1) K. Toei, et al., Nitrosophenol and Nitrosonaphthol Derivatives as Reagents for the Spectrophotometric Determination of Iron and Determination of Micro-amounts in Waters with 2-Nitroso-5-dimethylaminophenol. Analyst. 1975;100:629-636.
2) N. Ohno, et al., Spectrophotometric Determination of Iron in Boiler and Well Waters by Flow Injection Analysis Using 2-Nitroso-5-(N-propyl-N-sulphopropylamino) phenol. Analyst. 1987;112:1127-1130.
3) I. Yoshida, et al., Studies of the Stabilities of Scandium, Yttrium, and Lanthanoid-ion Complexes of 2-Nitroso-5-(N-propyl-3-sulfopropylamino)phenol. Bull Chem Soc Jpn. 1988;61:2639-2640.
4) I. Yoshida, et al., Potentiometric Studies on the Binding Properties of Protons, Some Divalent and Tervalent Metal Ions with 2-Nitroso-5-(N-propyl-3-sulfopropylamino)phenol. Anal Sci. 1988;4:69-72.
Table 1 Spectral Data of Nitroso-PSAP-Metal Complex