Chemical Name: 6-(Biotinylamino)hexanoic acid N-hydroxysuccinimide ester
CAS: 72040-63-2
Appearance: White or slightly yellow powder
Purity: ≥95.0% (HPLC)
MW: 454.54, C20H30N4O6S
Storage Condition: -20ºC
Shipping Condition: ambient temperature
Product Description of Amine-Reactive Biotins
The
avidin-biotin system has many applications in immunology and
histochemistry. The interaction between avidin and biotin is remarkably
strong with a dissociation constant on the order of 10-15
M. Biotin is usually added to primary or secondary antibodies such as
anti-IgG and anti-IgM. After preparing the antigen-antibody complex with
the biotin-labeled antibody, colorimetric, or fluorometric detection of
the antigen is performed using enzyme or fluorescein-labeled avidin or
streptavidin. Succinimidyl ester biotins react with primary and
secondary amines, such as amino acids and proteins, at pH 7-9.
Succinimidyl ester reacts with free amine groups to create a stable
amide bond. Succinimidyl biotin
reagents must be dissolved in DMSO, DMF, or alcohol. Stock solutions
prepared with DMSO are stable for several months at -20ºC. Sulfo
succinimidyl biotin reagents are soluble in water, so there is no need
to use organic solvents such as DMF or DMSO. IgG prepared using biotin
with a longer spacer such as Biotin-(AC5)2-OSu or Biotin-(AC5)2-Sulfo-OSu, has a better signal-to-noise ratio. The longer spacer enables streptavidin or anti-biotin IgG to recognize biotin without structural inhibition. Therefore, Biotin-(AC5)2-OSu is utilized as the biotin labeling agent in the Biotin Labeling Kit-NH2.
Reaction Scheme