Amine Coupling Kit

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Amine Coupling Kit

Item #	Unit Size
A515-10	2 ml x 4

For Research Use Only Products

∼ Features ∼
- Immobilization of protein/peptides onto SAMs with terminal carboxylic acid
- Including all necessary reagents for activation, immobilization, and blocking
- Sufficient for 40 immobilizations

Application: Immobilization of protein and/or peptide to carboxylic acid groups

Kit contents:

Storage Condition: 0-5ºC
Shipping Condition: Ambient temperature

Description
Application

Product Description:
Amine and carboxylic acid coupling is one of the most common methods to immobilize a protein or a peptide through a covalent bound onto biosensor surface. Dojindo’s Amine Coupling Kit contains all the reagents and buffers necessary reagents and buffers for activation of carboxylic acid, protein immobilization, and blocking. Blocking solution included in the kit minimizes non-specific protein absorption to the surface by capping residual activated esters. Each kit is adequate for approximately 40 immobilizations.

Fig.1 A biosensor prepared by Amine Coupling Kit

Example of Solvents
100 mmol/L (NHS solution)： Dissolve NHS １ tube in Activation Buffer 1 mL in the kit
100 mmol/L (WSC solution)： Dissolve WSC 1 tube in Activation Buffer 1 mL in the kit

Published Paper:
Expression and assembly of active human cardiac troponin in Escherichia coli
MW Lassalle - Protein expression and purification, 2012