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Amine Coupling Kit

Item # Unit Size
A515-10
2 ml x 4

For Research Use Only Products

  • Immobilization of protein/peptides onto SAMs with terminal carboxylic acid
  • Including all necessary reagents for activation, immobilization, and blocking
  • Sufficient for 40 immobilizations

    Application: Immobilization of protein and/or
                          peptide to carboxylic acid groups




  • WSC
    :4 tubes
    Reaction buffer
    :10 ml x 1
    NHS
    :4 tubes
    Blocking Solution
    :20 ml x 1
    Activation buffer
    :20 ml x 1
      

    Storage Condition :
    0-5ºC
    Shipping Condition : Ambient temperature

    Product Description: Amine and carboxylic acid coupling is one of the most common methods to immobilize a protein or a peptide through a covalent bound onto biosensor surface.  Dojindo’s Amine Coupling Kit contains all the reagents and buffers  necessary reagents and buffers for activation of carboxylic acid, protein immobilization, and blocking. Blocking solution included in the kit minimizes non-specific protein absorption to the surface by capping residual activated esters. Each kit is adequate for approximately 40 immobilizations.



                                                                        Fig.1 A biosensor prepared by Amine Coupling Kit

    Kit contents:
    Unite: 2 ml x 4
    Contents: WSC                            4 tubes
                     NHS                             4 tubes
                     Activation buffer         20 ml x 1
                     Reaction buffer           10 ml x 1
                     Blocking solution        20 ml x 1

    Example of Solvents
    100 mmol/L (NHS solution): Dissolve NHS 1 tube in Activation Buffer 1 mL in the kit
    100 mmol/L (WSC solution): Dissolve WSC 1 tube in Activation Buffer 1 mL in the kit

    Published Paper:
    Expression and assembly of active human cardiac troponin in Escherichia coli
    MW Lassalle - Protein expression and purification, 2012