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Biotin-SAM Formation Reagent

Item # Unit Size
B564-10
1 µmol x 3

For Research Use Only Products

- Simple preparation of Biotin-SAM on gold surface
- Efficient Immobilization of Avidins
- Suppress unspecific binding with unknown proteins

Appearance: White or slightly yellowish solid

Storage Condition: 0-5°C
Shipping Condition: Blue Ice

Keyword: Self-Assembled Monolayers, SAM, SPR, QCM, biosensors, protein immobilization


Product Description
There are three ways to immobilize a protein on a Surface Plasmon Resonance (SPR) or Quartz Crystal Microbalance (QCM) biosensor consisting of Self-Assembled Monolayer (SAM); 1) Formation of covalent bond of amine functions on proteins and activated carboxy-SAM; 2) immobilize proteins by His-Tag through Ni-NTA-terminated SAM; 3) Immobilization of proteins through Biotin and Avidin interaction. Because the Biotin-Avidin formation is known as a facile reaction, it is widely utilized for immobilization of protein (Fig 1).

                                                    Fig.1 A biosensor construction by Biotin-SAM Formation Reagent





Fig. 2  Comparison of the amount of bound streptavidin, unspecific binding of FBS, bound biotinylated BSA between Biotin-SAM Formation Reagent and Company A.




Example of Solvents
1 mmol/L (ethanol solution):Dissolve 1 bottle in 1 mL ethanol

Published Paper:
Localized surface plasmon resonance sensors based on wavelength-tunable spectral dips
Emiko Kazuma and Tetsu Tatsuma, Nanoscale, 2014, 6, 2397 - 2405


An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing
K Nomura, SCB Gopinath, T Lakshmipriya, et. al, Nature, 2013,  


Evaluation of the Specific Adsorption of Biotinylated Microbubbles Using a Quartz Crystal Microbalance
T Muramoto, R Shimoya, K Yoshida et. al, Ultrasound in Medicine & Biology, 2014