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Biotin-SAM Formation Reagent

Item # Unit Size
1 µmol x 3

For Research Use Only Products

  • Simple preparation of Biotin-SAM on gold surface
  • Efficient Immobilization of Avidins
  • Suppress unspecific binding with unknown proteins

  • Storage Condition: 0-5°C
    Shipping Condition: ambient temperature

    Keyword: Self-Assembled Monolayers, SAM, SPR, QCM, biosensors, protein immobilization

    Product Description
    There are three ways to immobilize a protein on a Surface Plasmon Resonance (SPR) or Quartz Crystal Microbalance (QCM) biosensor consisting of Self-Assembled Monolayer (SAM); 1) Formation of covalent bond of amine functions on proteins and activated carboxy-SAM; 2) immobilize proteins by His-Tag through Ni-NTA-terminated SAM; 3) Immobilization of proteins through Biotin and Avidin interaction. Because the Biotin-Avidin formation is known as a facile reaction, it is widely utilized for immobilization of protein (Fig 1).

                                                        Fig.1 A biosensor construction by Biotin-SAM Formation Reagent

    Fig. 2  Comparison of the amount of bound streptavidin, unspecific binding of FBS, bound biotinylated BSA between Biotin-SAM Formation Reagent and Company A.

    Example of Solvents
    1 mmol/L (ethanol solution):Dissolve 1 bottle in 1 mL ethanol

    Published Paper:
    Localized surface plasmon resonance sensors based on wavelength-tunable spectral dips
    Emiko Kazuma and Tetsu Tatsuma, Nanoscale, 2014, 6, 2397 - 2405

    An angular fluidic channel for prism-free surface-plasmon-assisted fluorescence capturing
    K Nomura, SCB Gopinath, T Lakshmipriya, et. al, Nature, 2013,  

    Evaluation of the Specific Adsorption of Biotinylated Microbubbles Using a Quartz Crystal Microbalance
    T Muramoto, R Shimoya, K Yoshida et. al, Ultrasound in Medicine & Biology, 2014