Apoptosis is one of the programmed cell death, that plays an important role in maintaining the homeostasis and
developmental processes in both plants and animals. Any abnormal cells during the cytogenesis are eliminated
by apoptosis. For instance, tumor growth from cancer cells occurred in vivo is inhibited by induction of apoptosis.
However, apoptosis is not induced when the error occurs in tumor suppressor gene p53. Thus, the growth of
cancer cells has been found to proceed. Apoptotic cells can be identified based on the alteration of cellular
morphology as well as the alternation of biomedical changes.
As of today, various indicators have been established such as caspase activity variation, DNA fragmentation and phosphatidylserine transition on the cell surface-chromosomal. Annexin V stained cells are used to indicate cell
membrane changes that occur in the early stage of apoptosis. Once apoptosis is initiated, the phosphatidylserine
presents in the inner cell membrane migrates through the cell membrane of the lipid bilayer.
Annexin V specifically binds to phosphatidylserine in the presence of protein-dependent Ca ion. By using
fluorescent-labeled Annexin V, the apoptotic cells can be identified by flow cytometry or fluorescence microscopy.
Fig. 1 Fluorescent imaging of apoptosis induced cells
Jurkat cells were apoptosis induced with staurosporine with its concentration of 1 μg/ml at 37°C for 3.5 hours
and then observed under a fluorescent microscope.
Fig. 2 Flow cytometric analysis of apoptosis induced cells.
Jurkat cells were apoptosis induced with staurosporine with its concentration of 1μg/ml at 37°C for 3.5 hours and
then analyzed with a flow cytometer.
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