Product Description:
Apoptosis is one of the programmed cell death, that plays an important role in maintaining the homeostasis and
developmental processes in both plants and animals. Any abnormal cells during the cytogenesis are eliminated
by apoptosis. For instance, tumor growth from cancer cells occurred in vivo is inhibited by induction of apoptosis.
However, apoptosis is not induced when the error occurs in tumor suppressor gene p53. Thus, the growth of
cancer cells has been found to proceed. Apoptotic cells can be identified based on the alteration of cellular
morphology as well as the alternation of biomedical changes.
As of today, various indicators have been established such as caspase activity variation, DNA fragmentation and phosphatidylserine transition on the cell surface-chromosomal. Annexin V stained cells are used to indicate cell
membrane changes that occur in the early stage of apoptosis. Once apoptosis is initiated, the phosphatidylserine
presents in the inner cell membrane migrates through the cell membrane of the lipid bilayer.
Annexin V specifically binds to phosphatidylserine in the presence of protein-dependent Ca ion. By using
fluorescent-labeled Annexin V, the apoptotic cells can be identified by flow cytometry or fluorescence microscopy.
Experimental Data:
Fig. 1 Fluorescent imaging of apoptosis induced cells
Jurkat cells were apoptosis induced with staurosporine with its concentration of 1 μg/ml at 37°C for 3.5 hours
and then observed under a fluorescent microscope.
Fig. 2 Flow cytometric analysis of apoptosis induced cells.
Jurkat cells were apoptosis induced with staurosporine with its concentration of 1μg/ml at 37°C for 3.5 hours and
then analyzed with a flow cytometer.
1) Anti-mitotic chemotherapeutics promote apoptosis through TL1A-activated death receptor 3 in cancer cells.
Cell Research, 2018,28,544 – 555
2) Nanoenzyme-Augmented Cancer Sonodynamic Therapy by Catalytic Tumor Oxygenation
ACS Nano,2018, 12, 3780?3795
3) Bacteria-Driven Hypoxia Targeting for Combined Biotherapy and Photothermal Therapy
ACS Nano,2018,12 (6), pp 5995–6005
4) Tumor-Specific Multiple Stimuli-Activated Dendrimeric Nanoassemblies with Metabolic Blockade Surmount Chemotherapy Resistance.
ACS Nano,2017,11(1),416-429.
5) Near Infrared-Guided Smart Nanocarriers for MicroRNA-Controlled Release of Doxorubicin/siRNA with Intracellular ATP as Fuel.
ACS Nano,2016,10(3),3637-47
6) Noninvasive small-animal imaging of galectin-1 upregulation for predicting tumor resistance to radiotherapy.
Biomaterials, 2018,158,1-9
7) Cytotoxicity of guanine-based degradation products contributes to the antiproliferative activity of guanine-rich oligonucleotides.
Chemical Science,2015,6,3834-3836
8) A Novel Strategy through Combining iRGD Peptide with Tumor-Microenvironment-Responsive and Multistage Nanoparticles for Deep Tumor Penetration.
ACS Appl.Mater.Interfaces, 2015,7 (49),27458–27466
9) Trifolium Repens Blocks Proliferation in Chronic Myelogenous Leukemia via the BCR-ABL/STAT5 Pathway.
Cells, 2020, 9 (2), 379