Chemical Name: 3-[4-(Perylenylphenylphosphino)phenoxy]propyltriphenylphosphonium iodide
Purity: ≥80.0% (HPLC)
MW: 882.74, C53H41IOP2
Storage Condition: 0-5ºC, protect from light
Shipping Condition: ambient temperature
a cell-membrane-permeable probe, Perylene-based dye. It specifically localizes
in mitochondria due to the triphenylphosphonium moiety introduced.
excitation and emission wavelength of MitoPeDPP are 452 nm and 470 nm,
respectively, the probe can be applied for lipophilic peroxide imaging in
*This probe has been
developed by Dr. Shioji
et al. at
Fukuoka University, Department of Chemistry
Peroxides Detection in Mitochondria (cell line used: HepG2 cell)
A: MitoPeDPP stained Mitochondria with t-BHP treatment
B: MitoRed stained Mitochondria
C: Merged Image (A/B)
of MitoPeDPP as a Peroxides Probe
The MitoPeDPP is
mitochondria (A) but not with ROS and RNS (B).
A: MitoPeDPP stained cells with t-BHP treatment (t-BHP) and without (control).
B: MitoPeDPP stained cells with ROS or RNS exposure.
*As ROS generators,
PMA (O2-・), NOC7(NO), and SIN-1(ONOO-) were used in the
SIN-1: 3-(Morpholinyl)sydnonimine, hydrochloride
1) K. Shioji K, Y. Oyama, K. Okuma and H. Nakagawa, "Synthesis and properties of fluorescence probe for detection of peroxides in mitochondria.", Bioorg Med Chem Lett., 2010, 20, (13), 3911.
2) K. Shioji, Y. Ogawa, H. Iwashita, Y. Oyama, N. Nagahora, K. Okuma and H. Nakagawa, ‘’Fluorescence imaging of accumulated lipid peroxidation in mitochondria by oxidative stress’’, Bioorg. Med. Chem, submitted.
3) S. Oka, J. Leon, K. Sakumi, T. Ide, D. Kang, F. M. LaFerla and Y. Nakabeppu, "Human mitochondrial transcriptional factor A breaks the mitochondria-mediated vicious cycle in Alzheimer’s disease", cientific Reports ., 2016, DOI: 10.1038/srep37889 , .
4) S. Nakamura, A. Nakanishi, M. Takazawa, S. Okihiro, S. Urano and K. Fukui, "Ionomycin-induced calcium influx induces neurite degeneration in mouse neuroblastoma cells: Analysis of a time-lapse live cell imaging system", Free Radical Research., 2016, 50, (11), 1214.