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Cytotoxicity LDH Assay Kit-WST

Item # Unit Size
CK12-01
100 tests
CK12-05
500 tests
CK12-20
2000 tests

For Research Use Only Products

∼ Feature ∼

  1. Can be used with and without transferring supernatant
  2. Stable Working Solution (6 months at 0-5 °C)
  3. No need to freeze the Working Solution

Increase your Cytotoxicity Data Reliability With Cytotoxicity LDH Assay Kit-WST

MSDS



Detection Principle

Lactate dehydrogenase(LDH) is an enzyme that presents in almost cell types and it catalyzes the oxidation of lactate to pyruvate in the presence of co-enzyme NAD+. Once cells are impaired by stress, injuries, chemicals, or intercellular signals, LDH is rapidly released from the cell membrane. Thus, the measurement of the amount of released LDH from cells is one of the major methods to assess the cell death. Since Dojindo's Cytotoxicity LDH Assay Kit-WST neither reflects the activity of living cells nor is harmful to cells, it allows the assay to perform in wells containing both viable and damaged cells.



Choose between Two Procedures

Cytotoxicity LDH Assay Kit-WST can be applied with and without supernatant transferring. Please choose suitable method for your experiment.




Stable Working Solution

Working Solution is stable for 6 months under refrigerated conditions. Therefore, after the preparation, Working Solution can be used as a ready-to-use solution at any time during this period.

Experimental Examples


To obtain an accurate result in cytotoxicity assay, the samples are measured by different principles. Cell Counting Kit-8 (Product Code: CK04) measure NADH in living cells and LDH assay measure released LDH from dead cells. According to these results, the living cells decreased and dead cells increased with higher concentration of toxicant.


Storage Condition: 0-5°C

Shipping Condition: ambient temperature

Required Equipment and Materials:
Microplate reader with 490 nm filter, CO2 incubator,
96-well tissue culture plate(flat bottomed), 20 μl, 100-200 μl multi-channel pipette


Kit Contents by Unit Size

Contents of the Kit: 100 tests
Dye Mixture :1 tube Assay Buffer :11 ml, 1 tube
Lysis Buffer :1.1 ml, 1 tube Stop Solution :5.5 ml, 1 tube


Contents of the Kit: 500 tests
Dye Mixture :1 tube Assay Buffer :55 ml, 1 tube
Lysis Buffer :5.5 ml, 1 tube Stop Solution :27.5 ml, 1 tube


Contents of the Kit: 2000 tests
Dye Mixture :4 tube Assay Buffer :55 ml, 4 tube
Lysis Buffer :5.5 ml, 4 tube Stop Solution :27.5 ml, 4 tube

Stability of Working Solution


Reconstituted Working Solution has higher stability than competitor's kit, eliminating the additional time to prepare the Working Solution for each assay.


Comparison of Working Solution Stability


Linearity


Dojindo's Cytotoxicity LDH Assay Kit-WST has higher sensitivity than competitor's kits


Cytotoxicity of mitomycin C using HeLa cells

Culture medium: MEM, 10% FBS

Incubation: 37C, 5% CO2, 48 hours

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