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Cytotoxicity LDH Assay Kit-WST

Item # Unit Size
100 tests
500 tests
2000 tests

For Research Use Only Products

∼ Feature ∼
- Can be used with and without transferring supernatant
- Stable Working Solution (6 months at 0-5 °C)
- No need to freeze the Working Solution

Storage Condition: 0-5°C
Shipping Condition: ambient temperature

Required Equipment and Materials:
Microplate reader with 490 nm filter, CO2 incubator, 96-well tissue culture plate(flat bottomed), 20 μl, 100-200 μl multi-channel pipette

Kit Contents by Unit Size:
100 tests(CK12-01) 500 tests(CK12-05) 2000 tests(CK12-20)
Dye Mixture 1 tube 1 tube 4 tube
Assay Buffer 11 ml, 1 tube 55 ml, 1 tube 55 ml, 4 tube
Lysis Buffer 1.1 ml, 1 tube 5.5 ml, 1 tube 5.5 ml, 4 tube
Stop Solution 5.5 ml, 1 tube 27.5 ml, 1 tube 27.5 ml, 4 tube


Detection Principle

Lactate dehydrogenase(LDH) is an enzyme that presents in almost cell types and it catalyzes the oxidation of lactate to pyruvate in the presence of co-enzyme NAD+. Once cells are impaired by stress, injuries, chemicals, or intercellular signals, LDH is rapidly released from the cell membrane. Thus, the measurement of the amount of released LDH from cells is one of the major methods to assess the cell death. Since Dojindo's Cytotoxicity LDH Assay Kit-WST neither reflects the activity of living cells nor is harmful to cells, it allows the assay to perform in wells containing both viable and damaged cells.

Choose between Two Procedures

Cytotoxicity LDH Assay Kit-WST can be applied with and without supernatant transferring. Please choose suitable method for your experiment.

Assay Procedure

[Optimization of cell concentration]

[Homogeneous Assay]

[Non-homogeneous Assay]

Stable Working Solution

Working Solution is stable for 6 months under refrigerated conditions. Therefore, after the preparation, Working Solution can be used as a ready-to-use solution at any time during this period.

Experimental Examples

To obtain an accurate result in cytotoxicity assay, the samples are measured by different principles. Cell Counting Kit-8 (Product Code: CK04) measure NADH in living cells and LDH assay measure released LDH from dead cells. According to these results, the living cells decreased and dead cells increased with higher concentration of toxicant.

Stability of Working Solution

Reconstituted Working Solution has higher stability than competitor's kit, eliminating the additional time to prepare the Working Solution for each assay.

Comparison of Working Solution Stability


Cytotoxicity of mitomycin C using HeLa cells

Culture medium: MEM, 10% FBS

Incubation: 37C, 5% CO2, 48 hours

1) S. F. Jin, H. L. Ma, Z. L. Liu, S. T. Fu, C. P Zhang, Y. He, "XL413, a cell division cycle 7 kinase inhibitor enhanced the anti-fibrotic effect of pirfenidone on TGF-β1-stimulated C3H10T1/2 cells via Smad2/4.", Exp Cell Res., 2015, 339, (2), 289.
2) S. Watanabe, C. S. Moniaga, S. Nielsen, M. Hara-Chikuma, "Aquaporin-9 facilitates membrane transport of hydrogen peroxide in mammalian cells.", Biochem Biophys Res Commun ., 2016, 471, 191.
3) L. Wu, T. Oshima, J. Shan, H. Sei, T. Tomita, Y. Ohda, H. Fukui, J. Watari, H. Miwa , "PAR-2 activation enhances weak acid-induced ATP release through TRPV1 and ASIC sensitization in human esophageal epithelial cells.", Am J Physiol Gastrointest Liver Physiol ., 2015, 309, G695.
4) D. Zhou, B-H Li, J. Wang, Y-N Ding, Y. Dong, Y-W Chen and J-G Fan, "Prolyl Oligopeptidase Inhibition Attenuates Steatosis in the L02 Human Liver Cell Line.", PloS ONE., 2016, 11, (10), e0165224.
5) T. Fukami, A. Iida, K. Konishi, M. Nakajima , "Human arylacetamide deacetylase hydrolyzes ketoconazole to trigger hepatocellular toxicity", Biochem. Pharmacol., 2016, doi:10.1016/j.jphs.2016.07.007.
6)Y. Takanezawa, R. Nakamura, Y. Sone, S. Uraguchi and M. Kiyono, "Atg5-dependent autophagy plays a protective role against methylmercury-induced cytotoxicity", Toxicol. Lett., 2016, 262, 135.
7) M. Tanaka, M. Yoneyama, T. Shiba, T. Yamaguchi, K. Ogita, "Protease-activated receptor-1 negatively regulates proliferation of neural stem/progenitor cells derived from the hippocampal dentate gyrus of the adult mouse ", J Pharmacol Sci., 2016, doi:10.1016/j.jphs.2016.05.005.
8) S. Wakatsuki, A. Furuno, M. Ohshima, and T. Araki, "Oxidative stress-dependent phosphorylation activates ZNRF1 to induce neuronal/axonal degeneration", J Cell Biol., 2015, 211, (4), 881.
9) A. Ishijima, K. Minamihata, S. Yamaguchi, S. Yamahira, R. Ichikawa, E. Kobayashi, M. Iijima, Y. Shibasaki, T. Azuma, T. Nagamune & I. Sakuma, "Selective intracellular vaporisation of antibody-conjugated phase-change nano-droplets in vitro", Scientific Reports ., 2017, DOI: 10.1038/srep44077.
10) N. Li, W. Zhang, M. Khan, L. Lin, JM. Lin, "MoS2-LA-PEI nanocomposite carrier for real-time imaging of ATP metabolism in glioma stem cells co-cultured with endothelial cells on a microfluidic system", Biosens Bioelectron., 2017, 99, (2018), 142.
11) Y. Sakurai, W. Mizumura, M. Murata, T. Hada, S. Yamamoto, K. Ito, K. Iwasaki, T. Katoh, Y. Goto, A. Takagi, M. Kohara, H. Suga, and H. Harashima, "Efficient siRNA delivery by lipid nanoparticles modified with a non-standard macrocyclic peptide for EpCAM-targeting", Mol. Pharm.., 2017, 10.1021/acs.molpharmaceut.7b00362.
12) K. Hattori, K. Nakadate, A. Morii, T. Noguchi, Y. Ogasawara, K. Ishii., "Exposure to nano-size titanium dioxide causes oxidative damages in human mesothelial cells: The crystal form rather than size of particle contributes to cytotoxicity.", Biochem. Biophys. Res. Commun.., 2017, 10.1016/j.bbrc.2017.08.054.
13) H. Sakai, S. Tabata, M. Kimura, S. Yabe, Y. Isa, Y. Kai, F. Sato, T. Yumoto, K. Miyano, M. Narita and Y. Uezono, "Active Ingredients of Hange-shashin-to, Baicalelin and 6-Gingerol, Inhibit 5-Fluorouracil-Induced Upregulation of CXCL1 in the Colon to Attenuate Diarrhea Development", Biol. Pharm. Bull.., 2017, 40, (12), 2134.
14) R. Watanabe, T. Kurose, Y. Morishige and K. Fujimori, "Protective Effects of Fisetin Against 6-OHDA-Induced Apoptosis by Activation of PI3K-Akt Signaling in Human Neuroblastoma SH-SY5Y Cells.", Neurochem. Res.., 2018, 43, (2), 488-499.
15) M. Akimoto, R. Maruyama, Y. Kawabata, Y. Tajima and K. Takenaga, "Antidiabetic adiponectin receptor agonist AdipoRon suppresses tumour growth of pancreatic cancer by inducing RIPK1/ERKdependent necroptosis", Cell Death Dis., 2018, 9, 804.

[Optimization of cell concentration]

[Homogeneous Assay]

[Non-homogeneous Assay]

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