Mitochondria Research

	Mitophagy
Reagent	Mtphagy Dye	Keima-Red
Principle	Mtphagy Dye (included in Mitophagy Detection Kit) is a pH-sensitive fluorescent probe that accumulates in mitochondria and emits red fluorescence due to acidic conditions in a lysosome.	A pH-sentitive ratiometric fluorescent protein. The excitation spectrum changes accoring to pH. This protein shows high fluorescence ratio (Ex. 550 nm/440 nm) values in a lysozome.
Fixed cell staining	-	-
Live-cell staining	Yes	Yes
Fixation after live-cell staining	-	-
Staining time	> 30 min	-
Ex / Em	530 / 700	440, 550 / 620
Product code	MD01 , MT02	-

	Lipophilic peroxide	Singlet oxygen	Superoxide
Reagent	MitoPeDPP	Si-DMA	MitoSOX
Principle	A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with lipophilic peroxides in mitochondria to emit fluorescence.	A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with singlet oxigen generated in mitochondria to emit red fluorescence.	A cell-permeant fluorescent probe that accumulates in mitochondria and reacts with superoxide generated in mitochondria to emit red fluorescence.
Fixed cell staining	-	-	-
Live-cell staining	Yes	Yes	Yes
Fixation after live-cell staining	-	-	-
Staining time	> 15 min	> 45 min	> 10 min
Ex / Em	452 / 470	644 / 670	510 / 590
Product code	M466	MT05	-

	Membrane potential		Iron ion (Fe2+)	Calcium ion (Ca2+)
Reagent	JC-1	TMRM, TMRE	Mito-FerroGreen	Rhod2-AM
Principle	A cell-permeant ratiometric fluorescent dye that accumulates in intact mitochondria due to the membrane potential. The excitation spectrum changes according to the mitochondria membrane potential.	Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. Diffusion of the probes occurs in a damaged mitochondria that has the decreased membrane potential.	A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with ferrous ion in mitochondria to emit green fluorescence.	A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with calcium ion in mitochondria to emit red fluorescence.
Fixed cell staining	-	-	-	-
Live-cell staining	Yes	Yes	Yes	Yes
Fixation after live-cell staining	-	-	-	-
Staining time	10-60 min	30-60 min	30 min	30-60 min
Ex / Em	Monomer: 514 / 529 J-aggregation: 585/590	550 / 575	505 / 535	553 / 576
Product code	MT09	-	M489	R002

	Mitochondria staining
Reagent	MitoBright LT series	MitoTracker series	DsRed	MitoRed	Rhodamine 123
Principle	Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential.	Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential.	A red fluorescent protein that binds to intact mitochondria with the use of a transfection reagent.	Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential.	Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential.
Fixed cell staining	-	-	-	-	-
Live-cell staining	Yes	Yes	Yes	Yes	Yes
Fixation after live-cell staining	-	-	-	-	-
Staining time	> 10 min	15-45 min	Expression in 8-12 hrs.	> 15 min	> 15 min
Ex / Em	493 / 508, 547 / 563, 643 / 663	490 / 516 ~ 644 / 665	558 / 583	560 / 580	507 / 529
Product code	MT10 , MT11 , MT12	-	-	R237	R233