Mitochondria Research

Mitochondria, which have important functions in cells, are not only a place for ATP production by oxidative phosphorylation, but their activity and dysfunction are closely related to cancer, senescence, and neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Therefore, understanding the state of mitochondria is particularly important.





Mitophagy


Target Mitophagy
Reagent Mitophagy Detection Kit Keima-Red
Priciple Mtphagy Dye (included in Mitophagy Detection Kit) is a pH-sensitive fluorescent probe that accumulates in mitochondria and emits red fluorescence due to acidic conditions in a lysosome. A pH-sensitive ratiometric fluorescent protein. The excitation spectrum changes according to pH. This protein shows high fluorescence ratio (Ex. 550 nm/440 nm) values in a lysosome.
Accumulating in mitochondria Yes
(Covalent bond)
-
(Expression protein)
Live-cell staining
(Fixation after live-cell staining)

(-)

(-)
Fixed cell staining - -
Ex/Em (nm) 530/700 550 & 440/620
Staining time*1 30 min~ *2
Product code MD01 -

*1 Suitable staining time depends on sample type and reagent concentration.
*2 It is necessary to construct a keima expression strain.



Oxidative Stress


Target Lipophilic Peroxide ROS Singlet oxygen Superoxide
Reagent MitoPeDPP ROS Assay Kit Si-DMA mtSOX Deep Red MitoSOX Red
Principle A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with lipophilic peroxides in mitochondria to emit fluorescence. A cell-permeant fluorescent probe that reacts with ROS generated in mitochondria to emit fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with singlet oxigen generated in mitochondria to emit fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and reacts with superoxide generated in mitochondria to emit fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and reacts with superoxide generated in mitochondria to emit fluorescence.
Accumulating in mitochondria Yes
(Electrostatic interaction)
- Yes
(Electrostatic interaction)
Yes
(Electrostatic interaction)
Yes
(Electrostatic interaction)
Live-cell staining
(Fixed Cell staining)

(-)

(-)

(-)

(-)

(-)
Fixed cell staining - - - - -
Ex/Em (nm) 452/470 505/525 644/670 540/670 510/580
Common filter set FITC FITC Cy5 TexasRed
Cy5
TRITC
Staining time*1 30 min~ 15 min~ 45 min~ 10 min~ 10 min~
Product Code M466 R252 MT05 MT14 -

*1 Suitable staining time depends on sample type and reagent concentration.



Mitochondrial Membrane Potential


Target MMitochondrial Membrane Potential
Reagent MT-1 JC-1
Priciple Cell-permeant fluorescent dyes accumulate in intact mitochondria due to the membrane potential. MT-1 is extremely photostable and more sensitive than JC-1 and can provide equivalent detection sensitivity to TMRE. A cell-permeant ratiometric fluorescent dye that accumulates in intact mitochondria due to the membrane potential. JC-1 forms aggregate (in healthy mitochondria) with red fluorescence. As membrane potential decreases, JC-1 becomes monomers, which shows in green fluorescence.
Live-cell staining
(PFA fixation after staing)

(✓)

(-)
Fixed cell staining - -
Ex/Em (nm) 530-560/570-640 Monomer: 514/529
J-aggregation: 585/590
Common filter set TRITC
TexasRed
Monomer: FITC
J-aggregation: TRITC; TexasRed
Staining time*1 30 min 10~60 min
Product code MT13 MT09

*1 Suitable staining time depends on sample type and reagent concentration.



Mitochondria Staining


Target Localization of mitochondria
Reagent MitoBright LT series MitoBright IM Red MitoTracker series Rhodamine 123
Principle Cell-permeant fluorescent dyes accumulate in intact mitochondria due to the membrane potential. Cell-permeant fluorescent dyes accumulate in intact mitochondria due to the membrane potential. Cell-permeant fluorescent dyes accumulate in intact mitochondria due to the membrane potential. Cell-permeant fluorescent dyes accumulate in intact mitochondria due to the membrane potential.
Live-cell staining
(PFA fixation after staining)

(✓)

(✓)

(✓)

(-)
Fixed cell staining - - - -
Combination with
immunostaining
- Conditionally -
Long-term retention × × ×
Ex/Em (nm) Green: 493/508
Red: 547/563
Deep Red:643/663
549/566 490/516 ~ 644/665 507/529
Common filter set Green: FITC TRITC - FITC
Red: TRITC
DEEP Red: Cy5
Staining time*1 10 min~ 30 min~ 15~45 min 15 min~
Product Code MT10  MT11  MT12 MT15 - R233

*1 Suitable staining time depends on the sample type and reagent concentration.



Metal Ion Detection


Target Calcium ion (Ca2+) Iron ion(Fe2+)
Reagent Rhod 2-AM Mito-FerroGreen
Priciple A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with calcium ion in mitochondria to emit red fluorescence. A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with ferrous ion in mitochondria to emit green fluorescence.
Accumulating in mitochondria
(Electrostatic interaction)

(Electrostatic interaction)
Live-cell staining
(PFA fixation after staing)

(-)

(-)
Fixed cell staining - -
Ex/Em (nm) 553/576 505/535
Common filter set TRITC FITC
Staining time*1 30~60 min 30 min~
Product code R002 M489

*1 Suitable staining time depends on the sample type and reagent concentration.



Application Products
Mitophagy Detection Mitophagy Detection Kit
Mitochondrial Phospholipid Peroxidase Detection MitoPeDPP
Mitochondrial Ferrous Ion Detection Mito-FerroGreen
Singlet oxygen specific fluorescent probe Si-DMA for Mitochondrial Singlet Oxygen Imaging
Mitochondrial Membrane Potential JC-1 MitoMP Detection Kit
MT-1 MitoMP Detection Kit
Mitochondria Staining MitoBright LT Red
MitoBright LT Deep Red
MitoBright LT Green
MitoBright IM Red for Immunostaining
-Cellstain- MitoRed
Mitochondrial Superoxide Detection mtSOX Deep Red – Mitochondrial Superoxide Detection